Validation of an ELISA-based screening assay for the detection of amphetamine, MDMA and MDA in blood and oral fluid

The use of amphetamine and 'ecstasy' (MDMA) has increased exponentially in many European countries since the late nineties, leading to a rapid growth in the number of clinical and forensic analyses. Therefore, a rapid screening procedure for these substances in biological specimens has become an important part of routine toxicological analysis in forensic laboratories. The objective of this study was to evaluate the Cozart amphetamine enzyme-linked immunosorbent assay (ELISA) for the screening of plasma samples and oral fluid samples (collected with the Intercept device). Authentic plasma samples from drivers (n=360) were screened, using an 1:5-fold dilution. True positive, true negative, false positive and false negative results were determined relative to the in-house routine GC-MS analysis. Samples consisted of 144 amphetamine-only positives, 141MDMA/MDA-only positives, and 74 negatives when using the limit of quantitation as the cut-off level for confirmation (10 ng/mL). Using these results, receiver operating characteristic (ROC) curves were generated and optimal cut-off values for the screening assay were calculated. Analysis showed that the ELISA is able to predict the presence of either amphetamine or *MDMA/MDA (*MDMA as its metabolite MDA) in plasma samples with 98.3% sensitivity and 100% specificity at a cut-off value of 66.5 ng/mL d-amphetamine equivalents. A similar analysis was conducted on 216 oral fluid specimens collected from a controlled double blind study. Subjects received placebo or a high (100 mg) or low (75 mg) dose of MDMA. Oral fluid samples were collected at 1.5 and 5.5h after administration. Combined results of the analysis of the high and low dose oral fluid samples indicated a screening cut-off of 51 ng/mL d-amphetamine equivalents with both a sensitivity and specificity of 98.6% (using a LC-MS/MS confirmation cut-off of 10 ng/mL). In conclusion, these data indicate that the Cozart AMP EIA plates constitute a fast and accurate screening technique for the identification of amphetamine and MDMA/MDA positive plasma samples and oral fluid specimens (collected with Intercept. It should be emphasized that method validation should be performed for each type of biological matrix.     

Marianne’s liberty in jeopardy? A French analysis on recent counterterrorism legal developments

ABSTRACT

This article analyses two recent French counterterrorist legislations (Law No. 2016–386 – hereafter OCT&F law and the Law No 2017–1510 – hereafter the OCT&Flaw) through the lens of distinct yet complementary theoretical frameworks. Combining the State of Exception thesis of Giorgio Agamben, the Enemy Penology as framed by Günther Jakobs as well as the more recent scholarship contributions on Pre-Crime, the article seeks to contribute to the scholarly debate on the use and the consequences of the use of criminal and administrative law in the fight against terrorism. In view of the numerous terrorist attacks that France has faced in recent years, the article aims to provide deeper knowledge of the French case by drawing substantially from the unfamiliar French scholarship. The article argues that the measures recently adopted seem to deepen the exceptional and pre-emptive logic in which potentially dangerous subjects have to be identified as “the enemy” as soon as possible in order to then be contained and dealt with.     

Learning By Doing: The Long-Term Impact of Experiential Learning Programs on Student Success

Abstract

As the advisers for the Model United Nations and Judicial Internship programs at our university, we have witnessed first-hand the benefits of simulations and experiential learning. Specifically, students are able to apply knowledge from other classes to real-world situations, all while honing their writing skills and communication skills, as well as their ability to analyze and synthesize information—skills that are critical to success in a wide variety of careers. This practical experience also gives students confidence as they prepare for and make decisions about their future career paths. While we have anecdotal evidence suggesting that these programs contribute to student success, we use survey data from former students to assess formally the longer-term impact of participating in these programs. We find that experiential learning programs contribute to student success by improving time to graduation and increasing the likelihood of attending graduate school or law school and/or finding employment after graduation. In addition, the students’ own assessments indicate that these programs instill numerous practical skills and provide insights that help prepare students for success in their future careers.     

‘New friends,easier partners and bigger brothers’: The influence of the emerging powers on agriculture and food security in Malawi

Emerging powers are credited with influencing development cooperation, but field-based studies incorporating the viewpoints of recipient developing country actors are scarce. This article analyses the presence and the material, ideational and institutional influence of assistance in agriculture and food security in Malawi by the governments of Brazil, India, China and South Africa. The findings suggest first that, despite a similar and powerful discourse linking these four states, in practice the emerging powers' approaches diverge to a large extent. Second, the material influence of these emerging powers is relatively limited and consequently so is their influence on Malawi's decision-making process with regard to development. Finally, perceptions about the emerging powers' potential contribution to addressing development challenges differ greatly between the Malawian government and the traditional donor community. This difference arises from limited coordination and cooperation on the one hand and the importance of ideational influence on the other.     

Smallholder Drip Irrigation in Burkina Faso: The Role of Development Brokers

Smallholder drip irrigation is widely held as a promising technology for water saving, poverty reduction and food security, despite a dearth of evidence of benefits to farmers, especially in sub-Saharan Africa. In this article, we document three development programmes promoting drip irrigation in Burkina Faso. Using actor network theory and insights from critical development studies, we show that development brokers play a key role in aligning interests, shaping activities and interpreting project outcomes. They are accountable towards each other rather than to farmers. This means that success is interpreted through development agencies lenses and with the intention of continuing involvement in future projects. Small farmers’ interests and uptake of the technology are of secondary importance.