灌服毒鼠强诱导大鼠细胞DNA的损伤研究

目的研究毒鼠强体内染毒后,毒鼠强对大鼠脑细胞、心肌细胞、淋巴细胞DNA的损伤作用。方法选择健康Sprague-Dawley大白鼠20只,分成5组,每组4只,采用灌胃方法使大鼠毒鼠强体内染毒,按0.2,0.1,0.05,0.01mg·kg-1制作大鼠毒鼠强中毒模型,并以灌服生理盐水的健康大鼠为对照,分离实验大鼠的淋巴细胞、心肌细胞和脑细胞,用彗星电泳的方法测定不同浓度毒鼠强中毒后的细胞DNA损伤。结果0.2,0.1,0.05,0.01mg·kg-1剂量组的毒鼠强均可引起大鼠的淋巴细胞、心肌细胞和脑细胞DNA损伤,均与对照组差异有显著性(P<0.01)。结论毒鼠强诱导体内细胞DNA损伤可能是毒鼠强毒性作用机制之一。     

Emerging Markets: Preferences,R isks,Performance...

The paper surveys the most important literature on emerging markets and their performance. Emerging market countries are defined here as the countries with low intuitional capacity in general, rather than the countries with particular economic characteristics and per capita income; although the latter is the predominant view in the current literature. The paper places particular importance on the legal system and legal order (compliance) in the transitional economies, stressing the importance of adequate regulation where even more advanced regulatory models, like market regulation, should not be totally excluded. Despite many common characteristics, emerging markets differ significantly one from another and it is very difficult, if really not impossible, to create one ‘general theory of emerging markets’ and its financial behaviour. Finally, the practice in the last decade or so, has proven that emerging markets are somewhat unpredictable and difficult to model.     

老年人保健品诈骗犯罪侦查研究——基于大数据背景

老年人保健品诈骗犯罪具有职业化、犯罪对象范围明确、犯罪手段多元、隐蔽且变异性强等特点。传统侦查方法面临受害人不能及时报案失去破案先机、侦查投入成本高、部门间协调机制不畅等困境。大数据技术可以满足人民群众快侦快破的新期待,在提供明确的侦查方向、实现犯罪深挖、助力控赃挽损等方面实现新突破。在大数据背景下,建立数据驱动侦查的大数据思维,加强警企合作并拓展整合数据来源,完善线上线下协同作战模式,提升民警的大数据侦查专业素养,有助于老年人保健品诈骗犯罪案件侦查机制的完善。     

DNA含量变化与死亡时间推断的研究进展

在法医学实践中,死亡经历时间的推断具有重要的实践价值,但其准确推断却一直是法医学实践的难题,因而也一直是法医学研究的热点。法医DNA技术是近年来发展起来的在分子水平进行死亡时间推断的新技术、新方法,同传统方法相比,具有很大的优势,本文对该技术提出的背景、发展、优缺点以及发展前景进行综述,着重对DNA含量变化与其相关性的研究进展进行讨论。     

改革开放以来党对社会主义本质实现的探索和完善

中国特色社会主义本质理论是在改革开放的实践中形成的,中国共产党人从社会主义建设实践的角度把社会主义本质的展开和实现看成一个动态的过程。社会主义本质的实现坚持以经济建设为中心,并以解放生产力、发展生产力为手段;社会主义本质的实现是一个过程。在这个过程中逐渐消灭剥削、消除两极分化,并坚持全面协调可持续的发展以实现社会主义的发展和繁荣;社会主义本质实现的目标是共同富裕,并在坚持科学发展和社会和谐的过程中不断促进人的全面发展。     

The theory of postmortem rigidity: the history and an original concept

The original theory of postmortem rigidity has been developed and substantiated based on the concept of postmortem muscular contracture. It is postulated that the unrestricted growth of Ca2+ concentration in myoplasm of contractile cells during the immediate postmortal period brings the actin-myosine complex to the force generation state without subsequent relaxation.     

In vitro production of GHB in blood and serum samples under various storage conditions

The in vitro production of GHB was observed in freshly collected, untreated whole blood samples using glass BD-Vacutainers and polypropylene S-monovettes. GHB concentrations were determined daily over a period of one week and after 3, 6 and 9 weeks again. Furthermore, the GHB concentration in 40 untreated random whole blood samples stored at 4°C for a longer period of time (10 samples 12 month, 10 samples 24 month and 20 samples 36 month) was also determined. For comparison, the in vitro production of GHB in freshly collected and prepared serum samples was observed. GHB serum concentrations were determined three times over a period of one week and once again after six weeks. Sample preparation was performed by means of methanolic extraction following the precipitation of whole blood and serum samples. A methanolic standard calibration was done in a low range of 0.005-0.1 μg/mL (LOD: 0.004, LLOQ: 0.013). For quantification a spiked blood bank serum with a determined GHB concentration of 0.09 μg/mL was used. Corrected calibrations in the range of 0.09-5.09 μg/mL were used (LOD: 0.08 μg/mL, LLOQ: 0.30 μg/mL), recovery: 91.3% (high level: 4.09 μg/mL) 50.5% (low level: 0.19 μg/mL). RESULTS: Relevant elevation of GHB was observed in all whole blood samples stored in liquid form (4°C or room temperature). In two of the 40 whole blood samples stored over a longer period of time at 4°C, GHB concentrations in the range of 13 μg/mL were even determined. These findings constitute grounds for caution. Even a GHB cut-off level of 5 μg/mL cannot be considered as "absolutely positive" proof of a case of exogenous administration, at least in untreated liquid blood samples in long time storage. However, no significant elevations of GHB were otherwise observed in any of the serum samples independently of storage temperature nor in the whole blood samples that were frozen for storage. CONCLUSIONS: The results suggest that the cut-off for exogenous GHB of 5 μg/mL could be lowered significantly, with the consequence of winning valuable time for the potential victim, but only if serum is collected for GHB determination or if the whole blood sample is frozen immediately after collection and the procedure well documented.     

Raman spectroscopy and microspectrophotometry of reactive dyes on cotton fibres: Analysis and detection limits

A collaborative study on Raman spectroscopy and microspectrophotometry (MSP) was carried out by members of the ENFSI (European Network of Forensic Science Institutes) European Fibres Group (EFG) on different dyed cotton fabrics. The detection limits of the two methods were tested on two cotton sets with a dye concentration ranging from 0.5 to 0.005% (w/w). This survey shows that it is possible to detect the presence of dye in fibres with concentrations below that detectable by the traditional methods of light microscopy and microspectrophotometry (MSP). The MSP detection limit for the dyes used in this study was found to be a concentration of 0.5% (w/w). At this concentration, the fibres appear colourless with light microscopy. Raman spectroscopy clearly shows a higher potential to detect concentrations of dyes as low as 0.05% for the yellow dye RY145 and 0.005% for the blue dye RB221. This detection limit was found to depend both on the chemical composition of the dye itself and on the analytical conditions, particularly the laser wavelength. Furthermore, analysis of binary mixtures of dyes showed that while the minor dye was detected at 1.5% (w/w) (30% of the total dye concentration) using microspectrophotometry, it was detected at a level as low as 0.05% (w/w) (10% of the total dye concentration) using Raman spectroscopy. This work also highlights the importance of a flexible Raman instrument equipped with several lasers at different wavelengths for the analysis of dyed fibres. The operator and the set up of the analytical conditions are also of prime importance in order to obtain high quality spectra. Changing the laser wavelength is important to detect different dyes in a mixture.     

社会管理概念的法规范分析

作为时下的一个焦点概念,"社会管理"极少在法规范意义上得以解析。通过检索可知,我国现行法规范中有3部法律及8部行政法规直接使用了社会管理概念,最典型的是《刑法》与《治安管理处罚法》。前者用整整一章规定了"妨害社会管理秩序罪",后者用一节规定了"妨害社会管理的行为和处罚"。分析表明,社会管理立法数量少,水平低;法规范中的社会管理概念是在狭义上使用,具有弱经济性、弱政治性乃至非经济性、非政治性的特征;主要是在消极语境中使用,管制色彩浓厚;法规范中的社会管理概念与社会管理创新语境下的社会管理概念有较大差别,不同法规范中的社会管理概念也有差异。社会管理法治化是社会管理创新的必由之路,它呼唤认真对待作为法律概念的社会管理。     

“90后”大学生思想道德行为特征解析

"90"后大学生的思想道德行为在生活方式上存在发展滞后的生活能力,标新立异的生活态度,贪恋当下的享受快乐;在行为方式上存在自我中心的行为取向,我行我素的行为特征,理性务实的行为作风;在思想理念上存在浓厚的民主理念,率性的自由精神,剧烈的价值冲突。对此应将思想政治教育与制度管理相结合、人文关怀与心理教育相结合、素质教育与能力培养相结合、挫折教育与激励教育相结合,以帮助其健康成长。