The EDNAP mitochondrial DNA population database (EMPOP) collaborative exercises: organisation, results and perspectives

This paper presents an overview of the organisation and the results of the collaborative exercises (CE) of the European DNA Profiling (EDNAP) Group's mitochondrial DNA population database project (EMPOP). The aim of the collaborative exercises was to determine whether uniformity of mtDNA sequencing results could be achieved among different laboratories. These were asked to sequence either the complete mtDNA control region or the two hypervariable regions HVI (16024-16365) and HVII (73-340) from DNA extracts, buccal swabs or bloodstains, proceeding in accordance with the protocol and strategies used in each individual laboratory. The results of the collaborative exercises were employed to identify possible sources of errors that could arise during the analysis and interpretation of mtDNA profiles. These findings were taken as a basis to tentatively make suitable arrangements for the construction of a high quality mtDNA database. One hundred fifty mtDNA profiles were submitted to the evaluating laboratory, and disaccording profiles were classified into four groups corresponding to the source of error: clerical errors, sample mix-ups, contaminations and discrepancies with respect to the mtDNA nomenclature. Overall, 14 disaccording haplotypes (16 individual errors) were observed. The errors included 10 clerical errors, 3 interpretation problems, 2 cases of sample mix-up and 1 case of point heteroplasmic mixture, where the 2 sequencing reactions brought inconsistent base calls. This corresponds to an error rate of 10.7% in a virtual mtDNA database consisting of the collaborative exercise results. However, this estimate is still conservative compared to conclusions drawn by authors of meanwhile numerous publications critically reviewing published mtDNA population databases. Our results and earlier published concerns strongly emphasize the need for appropriate safety regulations when mtDNA profiles are compiled for database purposes in order to accomplish the high standard required for mtDNA databases that are used in the forensic context.     

Environmental Federalism at Decade's End: New Approaches and Strategies

This article examines several recent trends in environmentalpolicy that may have important effects over the next decadeon federal-state relations and on the nation's environmentalquality. It focuses on the Environmental Protection Agency'sefforts to "reinvent" environmental regulation and to encouragecommunity-based environmental protection as a more effectiveand acceptable approach to environmental policy. We considerthe extent to which these new directions are likely to improverelations between the federal government and states, localities,and the private sector, and to achieve desired levels of environmentalprotection in the United States. The new approaches are highlypromising; yet they also face significant barriers to implementation.Congressional reform of the key statutes could lend much-neededsupport to such efforts.     

Mapping giant honey bee nests in Palawan,Philippines through a transdisciplinary approach

This article reports on a study that uses participatory mapping with indigenous Tagbanua honey hunters and gatherers to understand the spatial distribution of giant honey bees in a community forest in Palawan, Philippines. Through the use of global positioning system devices, digital cameras, and a solar home system as an electricity source, local collaborators mapped 31 bee nests from April to June 2015. This study provides a replicable long-term participatory methodology and promotes participatory learning and mutual knowledge creation. By combining applied sustainability research with local stakeholder participation, the article suggests that novel knowledge and solutions can aid sustainable rural development.     

Prevention Research in Schools

The August Vollmer Award Address is intended to focus on contributions to justice and on the recipient's research and policy experiences. This is a story of one person's career devoted mainly to developing effective collaborations between researchers and practitioners to produce useful knowledge. Her early work focused on delinquency prevention in schools and included evaluations of two different efforts aimed at altering school and classroom environments to reduce student misbehavior in Charleston, SC, schools, and an organization development intervention intended to reduce violence and related problem behaviors in Baltimore City, MD, schools. For many years, the author directed a project that provided research expertise to the Maryland Governor's Office of Crime Control and Prevention. She also partnered with public agencies to develop and implement randomized experiments to test the effectiveness of the Baltimore City Drug Treatment Court, the Strengthening Families Program in Washington, D.C., and afterschool programs in Baltimore County, MD. She is currently implementing a randomized trial of a gang prevention program in Philadelphia, PA, and a study of the effects of School Resource Officers in Florida and California.