Short tandem repeat (STR) genotyping of keratinised hair. Part 1. Review of current status and knowledge gaps

We present a review of the literature on procedures for obtaining short tandem repeat (STR) genotypes from keratinised hair, being either hair shaft or telogen phase (naturally shed) hairs without associated scalp, follicle or sheath cells. Both the hair shaft and the telogen hair club have been subjected to the DNA-degrading keratinisation process and are more likely to be found at a crime scene than anagen (plucked) or catagen phase hairs. We discuss human hair structure, the human hair growth cycle, the keratinisation process and their implications for DNA extraction procedures, PCR amplification strategies and the interpretation of STR genotypes. Knowledge gaps and areas requiring research are identified and are the subject of a second article in this series.     

Oral fluid collection: the neglected variable in oral fluid testing

The potential to use oral fluid as a drug-testing specimen has been the subject of considerable scientific interest. The ease with which specimens can be collected and the potential for oral fluid (OF) drug concentrations to reflect blood-drug concentrations make it a potentially valuable specimen in clinical as well as forensic settings. However, the possible effects of the OF collection process on drug detection and quantification has often been over looked. Several studies have documented that drug-contamination of the oral cavity may skew oral fluid/blood drug ratios and confound interpretation when drugs are smoked, insufflated or ingested orally. OF pH is predicted to have an effect on the concentration of drugs in OF. However, in a controlled clinical study, the effect of pH was less than that of collection technique. Mean codeine OF concentrations in specimens collected a non-stimulating control method were 3.6 times higher than those in OF collected after acidic stimulation. Mean codeine concentrations were 50% lower than control using mechanical stimulation and 77% of control using commercial collection devices. Several factors should be considered if a commercial OF collection device is used. In vitro collection experiments demonstrated that the mean collection volume varied between devices from 0.82 to 1.86 mL. The percentage of the collected volume that could be recovered from the device varied from 18% to 83%. In vitro experiments demonstrated considerable variation in the recovery of amphetamines (16-59%), opiates (33-50%), cocaine and benzoylecgonine (61-97%), carboxy-THC (0-53%) and PCP (9-56%). Less variation in collection volume, volume recovered and drug recovery was observed intra-device. The THC stability was evaluated in a common commercial collection protocol. Samples in the collection buffer were relatively stable for 6 weeks when stored frozen. However, stability was marginal under refrigerated conditions and poor at room temperature. Very little has been published on the efficacy of using IgG concentration, or any other endogenous marker, as a measure of OF specimen validity. Preliminary rinsing experiments with moderate (50 mL and 2 x 50 mL) volumes of water did not reduce the OF IgG concentration below proposed specimen validity criteria. In summary, obvious and more subtle variables in the OF collection may have pronounced effects on OF-drug concentrations. This has rarely been acknowledged in the literature, but should to be considered in OF drug testing, interpretation of OF-drug results and future research studies.     

Distribution and optical purity of methamphetamine found in toxic concentration in a civil aviation accident pilot fatality

Toxicological evaluation of postmortem samples collected from a pilot involved in a unique fatal civil aircraft accident is described in this paper. A one-occupant airplane was substantially damaged upon colliding with terrain in poor visibility. Remains of the pilot were found outside the aircraft. Pathological examination revealed multiple blunt force injuries and vascular congestion. The fluorescence polarization immunoassay disclosed 8.0 microg/mL amphetamines in urine. Gas chromatographic/mass spectrometric analyses determined the presence of methamphetamine (1.13 microg/mL in blood and 59.2 microg/mL in urine) and amphetamine (0.022 microg/mL in blood and 1.50 microg/mL in urine). Methamphetamine was distributed throughout the body, including the brain. The amount of methamphetamine in gastric contents was 575-fold higher than that of amphetamine. The (+)- and (-)-forms of methamphetamine were present in equal proportions in gastric contents. The methamphetamine concentration found in blood was in the range sufficient to produce toxic effects, causing performance impairment.     

Population affinities of 19th Century Cuban crania: implications for identification criteria in South Florida Cuban Americans

Identification criteria, specifically discriminant function formulae derived from traditional craniometrics, currently used in South Florida for Cuban Americans and other "Hispanic" groups, are unsuitable to provide adequate biological profiles due to complex biological histories as well as widely diverse geographic origins. Florida's total population is approximately 16 million (15,982,378) individuals. Of the total population 2.682,715, or 16.8%, are self-identified as "Hispanic". South Florida (herein defined as Miami-Dade, Broward and Collier Counties) is home to 60% of the total Hispanic population of Florida with 1,291,737 (48.15%) residing in Miami-Dade County. The Hispanic population of Miami-Dade County makes up 57.0% of the total population of 2,253,362. Each recognized sub-group of Hispanics (Mexican, Puerto Rican, and Cuban) includes its own geographic point-of-origin and population history. Cuban-Americans (arriving in the late 1950's and early 1960's) make up the largest sub-population of Florida's Hispanics in any county and in Miami-Dade number 650,601 or 51% of the total Latin population. Additionally, as in other agricultural states, Florida has a very large population of undocumented workers who primarily arrive from Texas and points south of the Straits of Florida. Thus the application of the available traditional craniometric and non-metric methods are not appropriate for South Florida's Latin population. To begin to address this issue in relation to South Florida's Cuban population, we present an analysis of cranio-facial shape variation in a 19th Century Cuban sample, 17th Century Spanish sample, a Precontact Cuban sample, and Terry Blacks using geometric morphometric methods. Significant biological shape differences and patterns of variation are observed among the groups. These results provide us with a context in which to begin to understand the biological variation of Cuban Americans, which will enable the development of identification criteria specific for this U.S. hybrid Hispanic community.     

Community size, heterogeneity and voter turnouts

Numerous studies have found a negative relationship between the closeness of an election, the size of the electorate and voter turnout. It is often claimed that this relationship supports the rational voter hypothesis, with closeness and size proxying for the decisiveness of a vote. We offer a different interpretation. Larger communities are more heterogeneous than smaller ones, and turnouts are inversely related to the heterogeneity of a community. We present empirical support for this hypothesis using data for voter turnouts in Norwegian school language referendums. Community size is found to have a negative effect on voter turnouts, even after accounting for the probability of a single vote being decisive and the linguistic dimension of this heterogeneity. Our findings question the adequacy of the turnout regression in testing the rational voter hypothesis, as neither a positive correlation between closeness and turnout, nor a negative correlation between size and turnout can be exclusively attributed to instrumental voting.     

Diversion from youth courts in five Asia Pacific jurisdictions: welfare or restorative solutions

Since the 1970s, there has been a trend to move away from punitive and retributive approaches to reintegrative practices. This article provides an account of how young offenders in five Asia Pacific jurisdictions are diverted from being prosecuted in the youth courts and of to where they are diverted. The five cases demonstrate that diversionary measures in the Australasian jurisdictions tend to be more restorative in their practices than are those in Chinese-dominated jurisdictions, which adopt welfare responses. It is concluded that although there is some progress in the areas of decarceration and delegalization, the diversion goals of decategorization and deprofessionalization in Chinese-dominated jurisdictions have yet to be achieved. Such diversionary strategies carry the risk that many more young people committing minor offences may be subject to more intrusive measures and disguised social control in the name of diversion, welfare, or the prevention of future offending.     

Parental opposition to deinstitutionalization

Parental opposition to the deinstitutionalization of mentally retarded persons is a growing, organized phenomenon which has recently emerged as a nationwide challenge to the deinstitutionalization movement. It is a challenge, however, which has been virtually ignored by key actors in the movement, including planners and implementers of deinstitutionalization schemes, systemic and individual advocates, and researchers. In order to begin to address and appropriately resolve the challenges posed by this phenomenon, an examination of it was undertaken in Nebraska using a tripartite conceptual framework which (1) historically described the national and state social-legal context within which parental opposition has emerged; (2) identified the bases of the opposition; and (3) recommended how these concerns could be addressed and appropriately resolved by offering directions for future research and policy analysis based upon applicable psychological theories and studies, and legal decisions and principles.American Association for the Advancement of Science-American Psychological Association Congressional Science fellow, Washington, D.C.     

THE STATES' ROLE IN LAND USE POLICY

Historically, land use had been an exclusively local function. By the early 1960s, a "quiet revolution" broke the local government monopoly over zoning and land use, with a larger state role. Those states which passed land use legislation soon were faced with substantial political opposition, especially from rural dwellers and developers. When Congress considered national land use legislation in the early 1970s. these same interests united in effective opposition.