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181.
目的建立单管一步甲基化可变位点(methylationvariableposition,MVP)分析技术一单管消化后PCR链融解曲线分析(post—digestionPCR—meltingcurveanalysis,PDP—MCA)。方法以文献报道的差异甲基化区(differentiallymethylatedregion,DMR)为模型,在MVP两侧设计一组解链温度各不相同的引物。应用FastDigest甲基化敏感性限制酶(methylation—sensitiverestrictionenzyme,MSRE),在同一反应管内顺次进行DNA的酶切、复合扩增和MCA检测.生成MCA图谱。同时用该方法和传统的MSRE—PCRMCA技术检测相同样品(外周静脉血、精液、阴道液各5份),比较两种方法检测结果,验证其可行性,并分析比较不同样品的MCA/HRM图谱。结果解链温度相差2℃以上的片段,MCA峰分离良好,复合扩增后可以用MCA技术检测。应用单管PDP—MCA技术,可以集酶切、扩增和检测三步于一管,在2h内得到与传统方法一致的特异性图谱和数据,并实现样品的快速分类鉴别。结论单管PDP-MCA技术可以实现多个MVP的单管、闭管检测,具有简便、快速、易于自动化等优点,可用于样品DNA甲基化差异的检测。 相似文献
182.
自2011年开始,长沙学院对一年级学生实施了大类培养改革方案,这类学生的年龄集中在1990年以后出生,即"90"后大学生,主要阐述及分析了"90"后大学生的思想特点,针对"90"后大学生新的思想特点以及国家培养具有较高综合素质与能力等创新型人才的目标,提出了政治辅导员应该采取的新的思想教育对策。 相似文献
183.
184.
Abstract Financial and market conditions in the 1990s caused a sharp increase in the housing debt (in constant dollars) of households now approaching or just past normal retirement age. Households now in middle age have also set new records for housing debt and will likely continue to carry high housing debt when they reach old age in 10 or 20 years. In the future, this housing debt burden is likely to lead to financial and housing adjustments that suggest a qualitative change in behavior when these households reach the later stages of their working life. Many will need to work longer to service housing debt. When facing a life‐cycle downturn in annual income, households will be increasingly motivated to tap into their home equity, both by borrowing, for those who stay in their homes, or by downsizing and liquidating some equity, for those who choose to move. 相似文献
185.
Conditional cash transfer (CCT) programmes aim to alleviate poverty through monetary and in-kind benefits, as well as reduce future incidence of poverty by encouraging investments in education, health and nutrition. The success of CCT programmes at reducing poverty depends on whether, and the extent to which, cash transfers affect adult work incentives. In this paper we examine whether the PROGRESA programme of Mexico affects adult participation in the labour market and overall adult leisure time, and we link these effects to the impact of the programme on poverty. Utilising the experimental design of PROGRESA's evaluation sample, we find that the programme does not have any significant effect on adult labour force participation and leisure time. Our findings on adult work incentives are reinforced further by the result that PROGRESA leads to a substantial reduction in poverty. The poverty reduction effects are stronger for the poverty gap and severity of poverty measures. 相似文献
186.
Jonathan Di John 《发展研究杂志》2013,49(6):961-986
This paper addresses one of the empirical claims of the ‘resource curse’ argument, namely that oil abundance raises the probability of political violence. I argue that the two main theoretical premises of the oil-civil-war link, rent-seeking and the rentier state model, fail to provide a convincing argument as to why oil economies are more vulnerable to the onset of civil war. I find that three often-mentioned mechanisms as to why oil economies succumb to political violence not convincing; that oil economies are poor economic performers; that oil economies generate high corruption; and that oil economies tend to produce authoritarian regimes. I also examine the empirical evidence on the oil-civil-war link and conclude that the results are not robust. I conclude with some policy implications. 相似文献
187.
为掌握安徽省鸽Ⅰ型副黏病毒病的流行特点及其与鸡新城疫各分离株的相互关系,对从安徽省不同鸽场鸽病料中分离的4株病毒(AH-2012、AH-2012-1、AH-2012-2、AH-2012-3),采用RT-PCR、血清学试验及基因序列测定等方法进行鉴定。结果显示,这些分离株均具有血凝性,并能使NDV标准阳性血清特异性抑制;PCR扩增出F基因特异性目的片段,其核苷酸序列与GenBank上登录的鸽Ⅰ型副黏病毒的同源性较高,相似性达94.8%;与传统的鸡源新城疫病毒标准株LaSota、F48E9的同源性较低,相似性约为80%;F基因编码蛋白的第112~117位氨基酸序列为R-R-Q-K-R-F。结果表明,这些毒株为鸽Ⅰ型副黏病毒,属于基因Ⅵ型。 相似文献
188.
Roberto Catanesi M.D. Vito Martino M.D. Chiara Candelli M.D. Ph.D. Giuseppe Troccoli M.D. Ignazio Grattagliano Psy.D. Giancarlo Di Vella M.D. Ph.D. Felice Carabellese M.D. 《Journal of forensic sciences》2013,58(5):1388-1392
The aim of this study is to identify protective and risk factors related to the development of posttraumatic stress disorder (PTSD) on a sample of survivors from a single plane crash. Eighteen survivors were examined 6 months following the event. The subjects all underwent psychiatric interviews, Clinician‐Administered PTSD Scale structured interviews, personality and cognitive tests. Only 38.9% of them presented with all of the symptoms of PTSD; 22.2% showed no symptoms for PTSD; remaining survivors exhibited emotional/affective symptoms related to the event. In addition to the severity of the traumatic event itself, other risk factors identified were the loss of a relative, the manifestation of depressive symptoms, and the severity of physical injuries sustained. Low levels of hostility and high levels of self‐efficacy represented protective factors against developing PTSD. 相似文献
189.
Claudio Terranova M.D. Marianna Tucci Ph.D. Daniela Sartore M.D. Fabiano Cavarzeran M.Sc. Laura Di Pietra M.D. Luisa Barzon A.P. Giorgio Palù F.P. Santo D. Ferrara F.P. 《Journal of forensic sciences》2013,58(5):1227-1232
The aim of this study was to analyze the connection between alcohol dependence and criminal behavior by an integrated genetic–environmental approach. The research, structured as a case–control study, examined 186 alcohol‐dependent males; group 1 (N = 47 convicted subjects) was compared with group 2 (N = 139 no previous criminal records). Genetic results were innovative, highlighting differences in genotype distribution (p = 0.0067) in group 1 for single‐nucleotide polymorphism rs 3780428, located in the intronic region of subunit 2 of the GABA B receptor gene (GABBR2). Some environmental factors (e.g., grade repetition) were associated with criminal behavior; others (e.g., attendance at Alcoholics Anonymous) were inversely related to convictions. The concomitant presence of the genetic and environmental factors found to be associated with the condition of alcohol‐dependent inmate showed a 4‐fold increase in the risk of antisocial behavior. The results need to be replicated on a larger population to develop new preventive and therapeutic proposals. 相似文献
190.
Kathryn Rudie Harrigan Maria Chiara Di Guardo Bo Cowgill 《The Journal of Technology Transfer》2017,42(5):1212-1233
Technological innovations enjoy synergies that vary in their speed and magnitude of impact, depending upon whether they are additive or multiplicative in nature. Additive-innovation synergies build incrementally on familiar technologies (as is reflected in the technologies built upon within their patents’ respective antecedents) and the duration of their effect is shorter-lived. Multiplicative-innovation synergies arise from combining greater proportions of diverse technologies and their effects have longer duration. The most-effective organizational-learning processes accompanying exposure to exotic technology streams via technological acquisition will occur if firms have properly invested in adaptive capacity to synthesize inventions using the unfamiliar knowledge. In the first tests of innovation synergies on firm performance, we find that technological novelty in patent content improves return on assets for firms that consistently invested in R&D. Using patent-content scores to characterize whether inventors have integrated greater proportions of exotic technological antecedents into their inventions (or not), we test the impact of innovation synergies on firms’ performance after technological acquisitions. Diversification posture (which could be an alternative explanation for performance differences) is negatively-correlated with innovation synergies in our results. 相似文献