Real-time PCR designs to estimate nuclear and mitochondrial DNA copy number in forensic and ancient DNA studies

We explore different designs to estimate both nuclear and mitochondrial human DNA (mtDNA) content based on the detection of the 5' nuclease activity of the Taq DNA polymerase using fluorogenic probes and a real-time quantitative PCR detection system. Human mtDNA quantification was accomplished by monitoring the real-time progress of the PCR-amplification of two different fragment sizes (113 and 287 bp) within the hypervariable region I (HV1) of the mtDNA control region, using two fluorogenic probes to specifically determine the mtDNA copy of each fragment size category. This mtDNA real-time PCR design has been used to assess the mtDNA preservation (copy number and degradation state) of DNA samples retrieved from 500 to 1500 years old human remains that showed low copy number and highly degraded mtDNA. The quantification of nuclear DNA was achieved by real-time PCR of a segment of the X-Y homologous amelogenin (AMG) gene that allowed the simultaneous estimation of a Y-specific fragment (AMGY: 112 bp) and a X-specific fragment (AMGX: 106 bp) making possible not only haploid or diploid DNA quantitation but also sex determination. The AMG real-time PCR design has been used to quantify a set of 57 DNA samples from 4-5 years old forensic bone remains with improved sensitivity compared with the slot-blot hybridization method. The potential utility of this technology to improve the quality of some PCR-based forensic and ancient DNA studies (microsatellite typing and mtDNA sequencing) is discussed.     

Thomas Paine's influence on the Girondin constitutional project of 1793

This article aims to investigate what may have been Thomas Paine's influence on the Girondin constitutional project presented by Marie-Jean-Antoine-Nicolas de Caritat Condorcet to the French National Convention, of which he was a member, on 14 and 15 February 1793. Indeed, the English radical was part of the nine-member committee, by the majority Girondin faction, in charge of drafting a new constitution for France. That project, which was accompanied by a Declaration of Rights, never came to fruition, but it has always been recognized as an important document for the interpretation of the evolutionary dynamics that characterized the French revolutionary period. Many of the provisions in the constitution recall Painite political thought, and studying this element can also be useful for understanding the links between the American and French Revolutions, given the fact that Paine actively participated in the experience of institutional upheaval initiated by the American colonists in 1775. The English thinker elaborated a political theory in his writings inspired by the most important democratic principles and based on respect and protection of fundamental human rights, all elements that, in their different mode of expression and declinations, can be found again in the Girondin constitutional charter of 1793.     

Forensic Interviews with Children in Scotland: A Survey of Interview Practices Among Police

The present study surveyed 91 police interviewers in Scottish police forces about their perceptions of how well they adhered to the Scottish Executive (2003) guidelines. Almost all respondents (97%) received the appropriate national training and overwhelmingly indicated (again 97%) that their training equipped them either quite, very, or extremely well for conducting their interviews. Not surprisingly, therefore, most interviewers (88%) believed that their interviews allowed them to obtain full and complete accounts of the events being investigated. However, aside from this positive self evaluation there are reasons to be concerned about some aspects of the interviews being conducted; 1) Most interviewers (78%) received no refresher training, 2) no interviewers received formal feedback about the quality of interviews that they conducted, 3) practice interviews were reportedly not included in most interviews, 4) the use of open-ended prompts were not widely used with 20% of interviewers indicating that they were rarely used, and 5) interviews are not currently being electronically recorded. These results are discussed with respect to the context of child interviewing in Scotland and recommendations for future training.     

Sexual assaults in Geneva between 2006 and 2010

In Geneva, all sexual assault victims are examined both by a gynaecologist and a forensic pathologist with special training in clinical forensic medicine. Between 2006 and 2010, 473 victims were examined following such an assault. Over the years, the number of sexual assaults rose steadily. Most victims were aged between 15 and 30 years. The majority of the assaults occurred at night and on the weekend and often happened at the place where the perpetrator or the victim lived. Usually, the offender acted alone and was known to the victim. Many victims hesitate to present for an examination, which makes it difficult to collect evidence. Penetration was usually vaginal and without the use of a condom. Injuries on the body or genitals were seen in only half of the cases for the first ones and in less than one third for the second ones. Quite often (at least in 42 % of the cases), the victim consumed alcohol before the assault and the use of drugs--especially cannabis--was not uncommon either.     

Factors Influencing the Filing of Complaints,Their Investigation,and Subsequent Legal Judgment in Cases of Sexual Assault

In Geneva, examination of victims of sexual assault is performed by a gynecologist and a medical examiner. 48% of the victims file a complaint and we wanted to investigate the factors leading to file a complaint, those leading the Prosecutor to go to trial, and those influencing a conviction. Between 2006 and 2012, 676 victims of sexual assault were investigated (averaged age 26 year, mean 22). Information on injuries, perpetrators, and circumstances of the assault was collected and analyzed. The attacker being the ex‐spouse or a friend and the presence of semen were factors leading to file a complaint. The assailant being a family member or ex‐spouse and the presence of genital/anal lesions were factors influencing the Prosecutor. The presence of nongenital lesions, the assailant being known by the victim, influenced conviction. This study shows that the medical examiner plays a vital role in the investigation of cases of sexual assault.     

The Law beneath Rights' Feet. Preliminary Investigation for a Study of the Charter of Fundamental Rights of the European Union

This article is meant as a philosophical preface to the study of the European Charter of Fundamental Rights. In particular, attention is focused on a particular legal positivistic reading of legislation as a political moment which would not allow for transcendental rights. This view is rejected by pointing out how much the notion of citizenship and consequently of fundamental rights is central for the democratic, and in some case even for the legal positivistic, celebration of legislation. In the last section a few conclusions are drawn as far as the scope of the Charter is concerned. In particular, any interpretation of it in the framework of the so–called regulatory paradigm (which gives up the democratic connection between deliberation and representation) is considered incoherent and self–defeating. In addition the principle of indivisibility of rights is evoked in defence of the validity of social rights within the Charter.     

行刑社会化的国际现状与我国发展的历史趋势

开展社区矫正试点工作,是我国对罪犯实行刑罚执行社会化改革的试点之一,也是我国现行刑罚执行制度的一个重要补充。国外刑罚执行社会化主要以教育刑思想为核心,保持较高的假释适用比率,挖掘开放式监狱的过渡功能,推行开放式处遇,保持罪犯矫正的社会参与,适用非监禁刑。我国监狱刑罚执行的重要目的之一在于改造罪犯,贯彻具有我国特色的行刑社会化,是探索完善刑罚执行制度,提高教育改造质量的需要。同时,也是中国刑事司法改革的重要内容。     

THE POLICY IMPACT OF NO-FAULT AUTOMOBILE INSURANCE

This study examines the impact of four different types of no-fault automobile insurance laws on the risk adjusted price of insurance. No-fault insurance plans are in operation in 23 states. Using data from 1976 to 1984 for 49 states, the study found that no-fault plans limiting litigation result in lower prices for automobile insurance. This relationship holds even under controls for several variables that also affect the price of automobile insurance.     

Unwanted children: Political violence and the cycle of protest in Italy, 1966–1973

Abstract. The article focuses on the cycle of protest that developed in Italy during the late 1960s and early 1970s. Some hypotheses on the evolution of the repertoires of action are tested with the aim of explaining the emergence of political violence during a cycle of protest. Newspaper-based data are presented on the proportional presence of violent forms of action, on the social and ideological groups involved in political violence, and on the grievances expressed during violent protests. The widespread political violence that developed in Italy in the early 1970s is explained as an internally differentiated strategic adaptation within the social movement sector, during a cycle of protests that was disorderly but far from violent.