Personal identification of cold case remains through combined contribution from anthropological, mtDNA, and bomb-pulse dating analyses

In 1968, a child's cranium was recovered from the banks of a northern Canadian river and held in a trust until the "cold case" was reopened in 2005. The cranium underwent reanalysis at the Centre for Forensic Research, Simon Fraser University, using recently developed anthropological analysis, "bomb-pulse" radiocarbon analysis, and forensic DNA techniques. Craniometrics, skeletal ossification, and dental formation indicated an age-at-death of 4.4 ± 1 year. Radiocarbon analysis of enamel from two teeth indicated a year of birth between 1958 and 1962. Forensic DNA analysis indicated the child was a male, and the obtained mitochondrial profile matched a living maternal relative to the presumed missing child. These multidisciplinary analyses resulted in a legal identification 41 years after the discovery of the remains, highlighting the enormous potential of combining radiocarbon analysis with anthropological and mtDNA analyses in producing confident personal identifications for forensic cold cases dating to within the last 60 years.     

Determination and validation of tetrodotoxin in human whole blood using hydrophilic interaction liquid chromatography-tandem mass spectroscopy and its application

A sensitive analytical method was developed for the quantitative determination of tetrodotoxin (TTX), a powerful sodium channel blocker, in human postmortem whole blood. The sample mixture was cleaned up using cation exchange SPE catridge after protein precipitation by methanol and then separated on a PC-HILIC (phosphorylcholine hydrophilic interaction liquid chromatography) column (150 mm × 2.0mm i.d., 5 μm) using a isocratic elution of 1% acetic acid and acetonitrile. The identification of TTX was performed on tandem mass spectrometry with electrospray ionization interface in positive ion mode. The retention time of voglibose (internal standard) and TTX was 5.1 and 6.0 min, respectively. TTX and internal standard (voglibose) were monitored and quantitated using the ion transitions: the respective precursor to product ion combinations, m/z 320/302 for TTX and m/z 268/92 for voglibose in the multiple reaction monitoring (MRM) mode. The recovery of TTX and voglibose was 61.4% and 62.8%, respectively and the good accuracy (97.7-103.9%), linearity (2-1200 ng/mL) and reproducibility were shown in this method. The limit of detection and limit of quantification were 0.32 ng/mL and 1.08 ng/mL, respectively. This method was applied in the case of three fishermen who were poisoned (including one death) by unknown fish on their boat in October 2010. In this case, the levels of TTX were 27.2, 30.0 and 29.7 ng/mL in heart blood, peripheral blood and serum of a victim, were 3.1 and 12.1 ng/mL in peripheral blood and 3.9 and 12.8 ng/mL in serum of two survivors, respectively.     

From the Inside/Out: Greene County Jail Inmates on Restorative ReEntry

The application of criminal justice sanctions is often misguided by a failure to recognize the need for a comprehensive approach in the transformation of offenders into law-abiding citizens. Restorative justice is a growing movement within criminal justice that recognizes the disconnect between offender rehabilitative measures and the social dynamics within which offender reentry takes place. By using restorative approaches to justice, what one hopes of these alternative processes is that the offenders become reconnected to the community and its values, something rarely seen in retributive models in which punishment is imposed and offenders can often experience further alienation from society. In this study, the authors wish to examine factors that contribute to failed prisoner reentry and reintegration and explore how restorative reintegration processes can address these factors as well as the needs, attitudes, and perceptions that help construct and maintain many of the obstacles and barriers returning inmates face when attempting to reintegrate into society.     

Detection and validated quantification of 21 benzodiazepines and 3 "z-drugs" in human hair by LC-MS/MS

A method for detection and quantification of 21 benzodiazepines and the pharmacologically related "z-drugs" in human hair samples was developed and fully validated using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). After methanolic and methanolic/aqueous extraction, the analytes were separated using two different LC-MS systems (AB Sciex 3200 QTRAP and AB Sciex 5500 QTRAP). Separation columns, mobile phases and MS modes for both systems were: Phenomenex Kinetex, 2.6 μm, 50/2.1; 5mM ammonium formate buffer pH 3.5/methanol, total flow 0.75 mL/min; electrospray ionization (ESI), multiple reaction monitoring (MRM), information dependent acquisition (IDA), enhanced product ion scan (EPI). The assays were found to be selective for the tested compounds (alprazolam, 7-aminoclonazepam, 7-aminoflunitrazepam, bromazepam, chlordiazepoxide, clonazepam, N-desalkylflurazepam, diazepam, flunitrazepam, flurazepam, alpha-hydroxymidazolam, lorazepam, lormetazepam, midazolam, nitrazepam, nordazepam, oxazepam, phenazepam, prazepam, temazepam, triazolam, zaleplon, zolpidem and zopiclone), all validation criteria were in the required ranges according to international guidelines, except for bromazepam. Matrix effects, and process efficiencies were in the acceptable ranges evaluated using the post-extraction addition approach. Lower limits of quantification were between 0.6 and 16 pg/mg of hair. The LC-MS/MS assay has proven to be applicable for determination of the studied analytes in human hair in numerous authentic cases (n=175).     

行政法官在发展社会政策中的作用

<正>行政法官有一种严重的、在一定意义上属于低人一等的情结。①相比于"真正的"法官,他们对自身的地位过度敏感。然而他们在社会中的作用却使其他法官黯然失色。尽管他们的价值常常受到成本—效益(cost effectiveness)的衡量,但最重要贡献却在于社会政策的发展方面。②鉴于行政国家的发展,他们已成为政策发展的关键角色。正如伟大智者詹姆斯·兰迪斯(James Landis)所言:"对行     

认知、认同与信仰:社会主义核心价值体系教育的三个维度及其推进路径

大力推进社会主义核心价值体系的宣传教育不仅关乎公民思想道德和精神文明建设状况的价值评判,更是塑造中华民族共有精神家园、实现文化强国与构建和谐社会的重要举措。应从认知、认同与信仰三个维度有步骤,分层次地展开与实施社会主义核心价值体系教育。要以情境化的思维和方式加强社会主义核心价值体系教育,这是推进公民社会主义核心价值体系有效认知、积极认同与信仰发生的重要路径,也是社会主义核心价值体系融入国民教育和精神文明建设全过程、贯穿现代化建设各方面的有效手段。     

China-Russia Ten-thousand-mile Tea Road

正From November 5 to 14,2014,a nine-member European NGO study group visited China at the invitation of CAFIU.The members of the group came from Russian and Austrian NGOs and think tanks,some of whom are senior China experts.To help them get a comprehensive idea of China’s development,in Beijing,Deputy SecretaryGeneral Zheng Yao briefed the group on China’s     

Must Internal Working Models be Internalized? A Case Illustrating an Alternative Pathway to Attachment

Internal working models of attachment are conceptualized as internalizations of interpersonal dynamics involving attachment—schemata derived from secure therapeutic relationships if not established in childhood. The potential for developing internal working models in the absence of adequate attachment history is demonstrated by the case of a dismissing (avoidantly-attached) client with Dissociative Identity Disorder (DID) abandoned in early childhood. Using the Collective Heart model (Krakauer 2001)—which targets posttraumatic misattributions of power and quickly challenges the client’s perceived brokenness and helplessness—the client formed an intimate bond with a formerly unconscious inner guidance resource experienced as a maternal attachment figure long before a robust therapeutic relationship was established. Verbatim case material illustrates autohypnotic interventions potentiating this “Wise Mother’s” guidance of the client’s identity development and mastery of relational tasks by mining and augmenting fragmentary attachment experiences. Theoretical and clinical implications of the evolution and function of the Wise Mother are discussed.     

CHINA＇S LATEST MARRIAGE LAW AMENDMENT AND FAMILY PROPERTY： TRADITION AND MODERNITY

China＇s latest Marriage Law amendment illustrates how tradition contributes to China＇s modernity. Traditionally, a house was a necessity for a marriage in China. This is because in ancient China, marriage secured the continuation of the family line and provision for ancestors. In modern China, the one child policy and soaring housing prices collectively force the ＂three families＂ to buy a house for the new couple. However, what happens when the couple divorces？ Shall the house then be considered community property？ The 2011 Judicial Interpretation of the Supreme People ＂s Court of Several Issues on the Application of the Marriage Law of the People＇s Republic of China, provides that where real estate is purchased by the parents of one party, and after the party＇s marriage is registered under the party＇s name, such real estate shall be deemed as a gift given by the parents to the party and the party＇s personal property. This interpretation represents a compromise between tradition and modernity.
The article will firstly introduce the marriage system in ancient China, illustrating that under the doctrine ofli, the real purpose of marriage was to be a bond of love between two （families of differenO surnames. Retrospectively, this secured observance of ancestral rituals in the ancestral temple, and prospectively, continuation of the family line. The house was a necessity for marriage because one of the six ceremonies required for marriage under li was the procession, and then welcome of the bride at the groom ＂s home. （Other ceremonies involved a matchmaker securing a proposal, matching auspicious birthdates, exchanging gifts between the bride＇s and groom＇s families, setting an auspicious wedding date, honoring ancestors and deities, and having an elaborate banquet）.
The article will then analyze the marriage law in the of Republic of China, in which even the post-dynasty marriage law adopted the western marriage system, the strong resistance of the old tradition forced the law-     

Genetic variation at nine STR loci in populations from the Philippines and Thailand living in Taiwan

In order to apply a set of nine STR loci and the amelogenin locus in forensic testing, we have performed a population study on individuals from the Philippines and Thailand living in Taiwan (273 Philippine and 146 Thai individuals were typed by commercially available kits and an automated sequencer). A total of 73 alleles for all systems for both populations could be observed in these two populations. No new intermediate fragments were found. Allele frequencies showed no deviation from Hardy-Weinberg equilibrium. The mean exclusion power (MEP) ranged from 0.327 (TPOX) to 0.706 (FGA), the discriminating power (DP) ranged from 0.790 (TPOX) to 0.963 (FGA) for Philippinos, MEP ranged from 0.247 (TPOX) to 0.723 (FGA), DP ranged from 0.761 (TPOX) to 0.968 (FGA) for Thais, the combined MEP is > 0.9988 and the combined DP is > 0.9999999993 for both Philippinos and Thais.