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Claire E.V. French Cynthia G. Jensen Susan K. Vintiner Douglas A. Elliot Susan R. McGlashan 《Forensic Science International Supplement Series》2008,178(1):1-6
There are a number of forensic cases in which the identification of the epithelial cell type from which DNA originated would provide important probative evidence. This study aimed to develop a technique using histological staining of fixed cells to distinguish between skin, buccal and vaginal epithelium. First, 11 different stains were screened on formalin-fixed, wax-embedded cells from five women. Samples were analysed qualitatively by examining staining patterns (colour) and morphology (absence or presence of nuclei). Three of the staining methods – Dane's, Csaba's and Ayoub-Shklar – were successful in distinguishing skin epithelial cells from buccal and vaginal. Second, cells were smeared directly onto slides, fixed with one of five fixatives and stained with one of the three stains mentioned above. Methanol fixation, coupled with the Dane's staining method, specific to keratin, was the only technique that distinguished between all three cell types. Skin cells stained magenta, red and orange and lacked nuclei; buccal cells stained predominantly orange–pink with red nuclei; while vaginal cells stained bright orange with orange nuclei and a blue extracellular hue. This staining pattern in vaginal cells was consistent in samples collected from 50 women aged between 18 and 67. Identification of cell type from unlabelled micrographs by 10 trained observers showed a mean success rate of 95%. The results of this study demonstrate that histological staining may provide forensic scientists with a technique for distinguishing between skin, buccal and vaginal epithelial cells and thus would enable more conclusive analyses when investigating sexual assault cases. 相似文献
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Douglas Lemke 《American journal of political science》2008,52(4):774-786
In order to evaluate the applicability of power politics theories of war and international stability to interactions among nonstate actors, I test hypotheses from power transition theory and from neorealist arguments about systemic polarity against the behavior of 20 state and nonstate actors in nineteenth‐century South America. I find considerable support for two of the three hypotheses tested and conclude that existing IR theory has more explanatory power within the empirical domain of nonstate relations than critics of such theory claim. 相似文献
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George D. Greenberg 《Policy Sciences》1981,13(2):153-181
This article examines the use of federal funds provided to state health departments under a grant consolidation of eight previously categorical health programs in Michigan, Pennsylvania, and Alabama in a comparative context. The primary question addressed is why the three states chose to describe their allocations of funds within the total health department budget differently, and what political, administrative, and bureaucratic factors explained the differences. Although certain factors were found to be at work in all states (e.g. each had an incentive to concentrate the reported use of federal funds to simplify federal audits) these factors combined with circumstances unique to each state to produce different expenditure patterns. After examining the experience of three states, general hypotheses are developed. For example, it is hypothesized that more volatile changes in allocations will result from grant consolidations in policy areas which do not address basic service needs. Finally the decision-making process with respect to block grant funds is characterized as one in which a small group of professionals determined allocations autonomously with relatively little input from interest groups or other actors within state government; nevertheless, the external political and administrative environment severely limited the possibilities of realistic choice in each of the three states studied.I wish to thank the National Center for Health Services Research which supported this study under grant HS 01495. I am indebted to the state health department officials in Alabama, Michigan, and Pennsylvania who made this research possible. I would also like to thank Leonard Robins, Janet Shikles, William Schmalzreid, Bruce Vladeck, John Kingdon and Robert Baitty for comments on an earlier draft. Of course the views expressed are my own and in no way reflect the positions of the Department of Health and Human Services. 相似文献
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Douglas A. Hibbs Jr. 《Public Choice》2008,137(1-2):1-10
Presidential election outcomes are well explained by just two objectively measured fundamental determinants: (1) weighted-average growth of per capita real personal disposable income over the term, and (2) cumulative US military fatalities owing to unprovoked, hostile deployments of American armed forces in foreign conflicts. The US economy weakened at the beginning of 2008 and average per capita real income growth probably will be only around 0.75% at Election Day. Moreover cumulative US military fatalities in Iraq will reach 4,300 or more. Given those fundamental conditions, the Bread and Peace model predicts a Republican two-party vote share centered on 48.2%. 相似文献
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Douglas L. Bland 《Canadian public administration. Administration publique du Canada》1987,30(4):527-549
Abstract: The Department of National Defence (dnd) and the Canadian Armed Forces (CF) have been the subject of many studies intended to elicit a better return from defence efforts. None of these studies have been at all comprehensive. The 1971 white paper, Defence in the 70s, introduced a new study aimed at “ensuring maximum effectiveness” in DND. The 1972 Management Review Group (MRC) report changed the entire administrative structure of DND and the CF, introduced new concepts for management, and shifted the power structure of the central bureaucracy. By the end of 1972, DND and the CF had a new set of principal actors, a new administrative structure, and a new policy process. To some the changes were more significant than previous unification reforms. The mrc report stands in sharp contrast to other studies because it has not been available to officials, scholars, or the public. Yet an awareness of die concepts that underlie the recommendations of the MRG is fundamental to understanding why the National Defence Headquarters exists and functions as it does today and why some issues are advanced over others. Sommaire: Le ministére de la Défense nationale (MDN) et les Forces armées cana-diennes (FC) ont fait l'objet de nombreuses “études” visant à accroître le rendement des efforts faits dans le domaine de la défense. Aucune de ces études n'est exhaustive. Le livre blanc de 1971, s'intitulant La défense dans les années 70, présentait une nouvelle étude dont l'objectif était “d'assurer une efficacité maximale” au sein du MDN. Le rapport de 1972 du Management Review Group (MRG) a modifyé toute la structure administrative du MDN et des FC, a introduit de nouveaux concepts de gestion et a entraîné un déplacement des pouvoirs de la bureaucratie centrale. Vers la fin de 1972, le MDN et les FC étaient dotés de nouveaux acteurs principaux, d'une nouvelle structure administrative et de nouvelles méthodes d'établissement de politique. Pour certains, ces changements ont été plus importants que les précédentes réformes d'unification. Contrairement aux autres études, le rapport du MRG n'a pas été mis à la disposition des responsables, des intellectuels ou du public. II est pourtant essentiel de con-naître les concepts sur lesquels s'appuient les recommandations du MRG si Ton veut comprendre pourquoi le Quartier général de la Défense nationale est ce qu'il est aujourd'hui, son mode de fonctionnement et les raisons pour lesquelles certaines questions ont pris le pas sur d'autres. 相似文献