The application of miniplex primer sets in the analysis of degraded DNA from human skeletal remains

A new set of multiplexed PCR primers has been applied to the analysis of human skeletal remains to determine their efficacy in analyzing degraded DNA. These primer sets, known as Miniplexes, produce shorter amplicons (50-280 base pairs (bp)) than standard short tandem repeat (STR) kits, but still utilize the 13 CODIS STR loci, providing results that are searchable on national DNA databases. In this study, a set of 31 different human remains were exposed to a variety of environmental conditions, extracted, and amplified with commercial and Miniplex DNA typing kits. The amplification efficiency of the Miniplex sets was then compared with the Promega PowerPlex 16 system. Sixty-four percent of the samples generated full profiles when amplified with the Miniplexes, while only 16% of the samples generated full profiles with the Powerplex 16 kit. Complete profiles were obtained for 11 of the 12 Miniplex loci with amplicon sizes less than 200 bp. These data suggest smaller PCR amplicons may provide a useful alternative to mitochondrial DNA for anthropological and forensic analysis of degraded DNA from human skeletal remains.     

The effects of the transfer of American educational technology on business students and a comparison with practicing managers in Lesotho,Africa, in relation to those in the United States

This research involved a comparative study of managers and advanced business students in the United States and in Lesotho, Africa. The objective was to identify the managerial beliefs of students in comparison to those of practicing managers both within and without their respective countries. This study found that Lesotho students, after having been exposed to American business school techniques, moderated their thinking processes to be more consistent with both U.S. managers and students than the practices of local managers practicing in their own country.     

Neuere Rechtsprechung zum Schächten aufgrund § 4a TierSchG

Das betäubungslose Schlachten warmblütiger Tiere durch Blutentzug, das sog. Schächten, sorgt seit den Entscheidungen des Bundesverfassungsgerichts im Jahr 2002 für erhebliches Aufsehen. Im Widerstreit zwischen Religionsfreiheit und Tierschutz hatte das Gericht der bis dahin eher restriktiven Genehmigungspraxis aufgrund von § 4a TierSchG eine Absage erteilt. Daraufhin gab der Gesetzgeber dem Tierschutz in Art. 20a GG n.F. Verfassungsrang. Seither müssen die unteren Verwaltungsbehörden besonders schwierige Einzelfallentscheidungen unter Berücksichtigung der verfassungsrechtlichen Wertentscheidungen treffen. Der folgende Beitrag erläutert die aufgetretenen Probleme und die Lösungsansätze der neueren Rechtsprechung. 1) Der Beitrag gibt die private Meinung des Verfassers wieder.     

Reconsideration of the agricultural transfer model

The cooperative extension system in American agriculture is widely viewed as the paradigmatic public sector technology transfer system. Empirical evidence supports the view that research and extension are complementary inputs in increasing agricultural productivity. Cooperative extension, however, does not perceive itself as a technology transfer system; but as an informal education system. Many participants at the federal, landgrant university, or county levels in cooperative extension would not recognize their work within a technology transfer framework. Moreover, many would object to such a characterization of their work. The differences in perspective is accounted for, in part, by the fact that agricultural extension is part of the larger system of cooperative extension. This system, which encompasses agriculture, home economics, 4-H and community and rural development, requires a more encompassing framework than technology transfer to provide an integrated rationals for its program elements. Within the technology transfer framework, the roles of the different components of the system across the states are both highly variable and changing. Based on site interviews in nine states, analysis is presented of the current and prospective roles of extension specialists and county agents.