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This paper describes a developmental validation study of three Miniplex sets covering 12 of the 13 CODIS loci. As these new sets will be used for the analysis of degraded and low level DNA, the validation studies were performed using 100-125 pg of DNA, the lowest input level at which peak balance, peak intensity, and allele consistency were stable. To demonstrate the applicability of the Miniplex sets to forensic casework, these validation studies were completed in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM). A range of tests were performed including studies of concordance with standard multiplex kits, sensitivity and reproducibility, and PCR amplification conditions. Additionally, studies of mixtures, nonhuman and environmentally degraded DNA, and simulated forensic samples were performed. Our results demonstrate that Miniplex STR amplification procedures are a robust and sensitive tool for the analysis of degraded DNA. 相似文献
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Rollen und Institutionen als symbolische Ordnung von Netzwerken 总被引:1,自引:0,他引:1
PD Dr. Jan A. Fuhse 《Berliner Journal für Soziologie》2012,22(3):359-384
Roles and institutions lead to the reduction of uncertainty in communication, and to a particular pattering of social networks. Cultural patterns for actorhood, for social relationships, and for patterns of relationships are conceptualized as ??relational institutions?? that structure social networks as cultural models. Roles mediate between the structure of social networks and institutionalized cultural patterns: On the one hand, they can emerge in small-scale network contexts and crystallize as long as the network structure persists. On the other hand, communication draws on institutionalized models to reduce its own complexity and uncertainty. Thus, relational institutions imprint social networks through role categories. In order to elaborate on this relational conception of institutions and roles, the text combines arguments from social network research, from the normative and the interpretive role concept, from philosophical anthropology and neo-institutionalism with the general perspective of relational sociology. 相似文献
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Dr LOTHAR HÖBELT 《议会、议员及代表》2013,33(1):183-189
SUMMARY In this article Lothar Höbelt compares the political role of the Liberal parties in the Hohenzollern and Habsburg empires after 1867. Both started from a similar position, by granting a retrospective indemnity to their governments for their unconstitutional actions prior to 1866, in the expectation of reinstating constitutional government in which they would have an active role. The article then analyses the reasons why, by 1879 these expectations had been disappointed, but this led to different outcomes in the politics of the two empires. In the Hohenzollern empire, the Liberals eventually split permanently into a party of National Liberals, prepared to cooperate with government, and a progressive wing that tended to be in opposition. In the Habsburg empire, the Liberals remained united in a largely unsuccessful attempt to enforce parliamentary responsibility on the government. It is then shown how this outcome relates to the different ethnic and political structures of the two empires. 相似文献
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