Macroscopic, histological and toxicological aspects of early Gammarus pulex scavenging

Two cases of scavenging postmortem freshwater shrimps (Gammarus pulex) are presented. We report the two first illustrated observations of cutaneous postmortem injuries inflicted by a G. pulex population, a small freshwater crustacean, on two non putrefied drowning victims, and we describe their particular histological features and their potential in forensic investigations.     

Seasonal and habitat abundance and distribution of some forensically important blow flies (Diptera: Calliphoridae) in Central California

Seasonal and habitat calliphorid abundance and distribution were examined weekly for two years (2001-2003) in Santa Clara County, California, using sentinel traps baited with bovine liver. Of the 34,389 flies examined in three defined habitats (rural, urban, and riparian), 38% of the total catch represented Compsomyiops callipes (Bigot) and 23% represented Phormia regina (Meigen). Other flies collected in this survey included Calliphora vomitoria (Linnaeus), Calliphora latifrons (Hough), Lucilia sericata (Meigen), Lucilia cuprina (Wiedemann), and Lucilia mexicana (Macquart), which is a new record for the area. Multivariate MANOVA and ANOVA (P ≤ 0.05) analysis indicate significant seasonal habitat preference for all fly species examined. This information may be used to identify potentially forensically impo rtant fly species within Santa Clara County, California.     

Development and validation of the AmpFℓSTR® Identifiler® Direct PCR Amplification Kit: a multiplex assay for the direct amplification of single-source samples

Abstract: The AmpF?STR^® Identifiler^® Direct PCR Amplification Kit is a new short tandem repeat multiplex assay optimized to allow the direct amplification of single‐source blood and buccal samples on FTA^® card without the need for sample purification and quantification. This multiplex assay has been validated according to the FBI/National Standards and SWGDAM guidelines. Validation results revealed that slight variations in primer concentration, master mix component concentration, and thermal cycling parameters did not affect the performance of the chemistry. The assay’s sensitivity was demonstrated by amplifying known amounts of white blood cells spotted onto FTA^® cards, and the assay’s specificity was verified by establishing minimal cross‐reactivity with nonhuman DNA. No effect on the age of the sample stored on the FTA^® substrate was observed and full concordance was established in the population study. These findings of the validation study support the use of the Identifiler^® Direct Kit for forensic standards and database samples genotyping.     

Sudden infant death syndrome: diagnostic practices and investigative policies, 2004

Using a 2004 population-based survey of all US medical examiner and coroner offices, we examined the characteristics of offices accepting an infant death case and calculated the percentage of offices that had death scene investigation or autopsy policies for the investigation of sudden unexpected infant death (SUID). We also calculated the percentage of offices that used and did not use sudden infant death syndrome (SIDS) as a cause of death, and we compared differences in characteristics among those offices.Of medical examiner and coroner offices, 52% did not report an infant death in 2004. Of the 7957 infant deaths reported, 43% occurred in jurisdictions that experienced 1 or 2 infant deaths. Of the offices that used SIDS as a classification, 34% did not have policies for conducting death scene investigations and autopsies for SUID. At least 5% of offices that reported an infant death did not use SIDS as a cause of death classification. These findings have important implications for understanding recent trends in SIDS and SUID. Supporting the implementation of national standards for investigating and certifying infant deaths could provide guidelines for consistent practices in medical examiner and coroner offices.     

Volatile organic compounds released by blowfly larvae and pupae: new perspectives in forensic entomology

To evaluate postmortem intervals (PMIs), one should take into account the determined age of necrophagous flies present on the cadaver. However, PMI determination needs further improvement, and rapid and accurate approaches have therefore to be developed. While previous studies have focussed on insect cuticular hydrocarbons, here we explore the volatile profile released by larvae and pupae of Calliphora vicina Robineau-Desvoidy (Diptera: Calliphoridae). We monitored changes in volatile compounds daily, by headspace solid-phase microextraction, followed by gas chromatography-mass spectrometry. Branched and unbranched hydrocarbons, alcohols, esters and acids were identified, and the volatile profile was shown to vary, in both composition and quantity, with the age of the larva/pupa under investigation. We concluded, based on the analysis of the released volatile organic compounds, that it is possible to increase the accuracy of the estimated PMI, through improved estimation of the age of blowflies present on the cadaver.     

Integrity management in post-1997 Hong Kong: Challenges for a rule-based system

Unlike some of its Western counterparts, where the first line of defence against corruption and unethical behavior has been a reliance on a public service ethos, the Hong Kong government has traditionally relied on strict compliance with rules and regulations in its successful drive against bureaucratic malpractices. Since 1997, however, a changing political, economic and administrative environment has also seen efforts to introduce a more value-based approach to integrity. This article examines the factors that have led to the modification of the Hong Kong government??s strategy and evaluates its success in the wider theoretical context of the relationship between a predominantly rule-based and a value-based approach to integrity management.     

基于纤维二次转移确定纤维屑作为示踪物质的探讨

目的 考察纤维屑在涉及二次转移案件中作为示踪物质的适用性.方法 根据一个实际案例展开,在该案中,犯罪嫌疑人涉嫌多起入室盗窃案.纤维开始分散于嫌疑人所用车辆,后被嫌疑人“携带”并分散到犯罪现场,最终这些纤维在勘查过程中被收集.设计完善了一个可用于散布纤维的便携式装置,并完成了一系列二次转移的相关实验.结果 设计的装置可在短时间均相的散布纤维,单次接触不能全部转移第一表面的纤维碎屑.只有1-15％的纤维会转移到代表犯罪现场的第三表面.实际转移的纤维数与包括比如表面的材质在内的各种因素都有关系.结论 在所选参数范围内,相对大量的纤维有可能转移到犯罪现场并在现场勘查过程中被提取.     

Studying the Arabs: Myths and metaphors

The relationship between knowledge and power is examined in this article in terms of the social science literature base on Arab countries. It is argued that knowledge imposes normative constraints on the exercise of power, and the exercise of power is dependent on the lack of knowledge. This is examined in terms of the knowledge base on Iraq and Palestine, the two countries in the Arab world most decimated in the post-World War II era by the exercise of external power politics. The study is organized in three phases: (1) delimiting the population of scholarly literature in the social sciences on the Middle East by country; (2) drawing representative samples of scholarly literature on Iraq and Palestine from this population; and (3) content analysis of the samples. Jacqueline S. Ismael is a professor of social work at The University of Calgary. She has published a number of articles and monographs on social change in the Middle East, as well as several works on Canadian social policy. She is co-author with Tareq Y. Ismael ofThe People’s Democratic Republic of Yemen: The Politics of Socialist Transformation (1986),Government and Politics in Islam (1986),Politics and Government in the Middle East andNorth Africa (1991), andThe Gulf War and the New World Order (1994). Her most recent work isKuwait: Dependency and Class in a Rentier State (1993). Tareq Y. Ismael is a professor of political science at The University of Calgary. He has written extensively on Middle East politics, the international relations of the Middle East, and ideology in the Arab world. His recent publications includeInternational Relations of the Contemporary Middle East (1986),Middle East Studies: International Perspectives on the State of the Art (1990),The Communist Movement in Egypt (1990),Politics and Government in the Middle East and North Africa (1991), andThe Gulf War and the New World Order (1994).     

Hyperuricemia in rats during acute carbon monoxide poisoning

The effect of carbon monoxide (CO) inhalation on plasma levels of uric acid and hypoxanthine in rats was investigated. Exposure to 3% CO caused respiratory arrest within about 2 minutes. The plasma uric acid level of CO-treated rats increased to 157% above that of ether-treated rats. When rats were exposed to 1% or 0.8% CO, the exposure periods until the onset of respiratory arrest were prolonged, and plasma uric acid levels at respiratory arrest were further elevated. Plasma uric acid levels at respiratory arrest increased with prolongation of the exposure periods. Under our experimental conditions, hypoxanthine or xanthine was not detected in plasma of CO-treated rats. These results are discussed in relation to the hyperuricemia in hemorrhagic shock or hypoxemia: CO-induced hyperuricemia can be attributed to the stimulated degradation of adenine nucleotides under tissue anoxia, and thus could be an excellent parameter of tissue anoxia.