A sandwich enzyme immunoassay for human pancreatic elastase III

To develop a method for the determination of pancreas injuries using a pancreas-specific antigen as a marker, human elastase III was purified from the pancreas by chromatographic methods. A rabbit anti-human elastase III antibody was prepared, and this antibody was confirmed using immunoblotting to react only with elastase III among proteins from the pancreas. A sensitive sandwich enzyme immunoassay for human elastase III was developed. The detection limit for human elastase III was 0.3 pg (10 amol) per assay. Proteins extracted from the pancreas showed the strongest response, whereas reactions of the other organs were less than the detection limit. These results suggest that a sandwich enzyme immunoassay for human elastase III is useful for the determination of pancreas injury.     

Interpersonal trust and disaffected high school youth

The study consisted of two parts. First, a new scale of interpersonal trust for adolescents was constructed using a representative sample of 70 students from a community high school. Second, the hypothesis was tested that disaffected youth compared to the more conventional would show less interpersonal trust. Two matched groups of 57 students each were employed for the second part of the study. Results indicate that the new scale is a reliable and valid measure. Correlates of the scale disclose that trust is associated with interpersonal distance, certain developmental factors, and the quality of parent-adolescent relations. Results from the second part of the study tend to confirm the hypothesis in that greater trust scores were found among students with more conventional beliefs.This study was partly supported by NIMH Grant No. MH9210, Ford Foundation Grant No. 69-0264B, and the Jessie and C. Clement Stone Foundation.Received his Ph.D. from the University of Kansas. Currently working on personality and clinical problems of adolescents and adults.Recieved his Ph.D. from the University of Michigan. Plans to continue his work of program development in the area of affect education in secondary schools.Received his Ph.D. from Harvard University. Current work is in the area of community mental health.