Screening for barbiturates in vitreous humor by the EMIT-st serum enzyme immunoassay

In 16 medical examiner's cases, which were found to be barbiturate-positive by thin-layer chromatographic screening of the liver, blood barbiturate concentrations were determined by gas chromatography. The corresponding vitreous humor samples were screened by the enzyme multiplied immunoassay technique, the EMIT-st serum barbiturate assay. By using the recommended dilution for detecting serum barbiturates, it was possible to detect barbiturates in vitreous humor at a toxic concentration. By using one fourth the amount of diluent, the barbiturates could be detected also at a therapeutic concentration. The EMIT-st assay proved to be useful in the screening for barbiturates in vitreous humor, a material that is readily available in forensic toxicology.     

Community Correction Officer Perceptions Of Ex-Offender Reentry Needs And Challenges

Research into community corrections officers’ perceptions of the needs of ex-offenders has largely been overlooked. While some empirical research has emerged regarding federal parole officers’ perceptions, it is conceptually incomplete. A gap in the literature remains regarding the concordance or discordance between offenders and community corrections officers’ perceptions of offender needs and the opportunities for success upon release. Using a sample of community corrections officers in Seattle, Washington, this research examined officer perception of ex-offender needs, the value officers’ placed on the specific needs, and the opportunities available for offenders to meet their needs. Differences between officers emerged including the finding that female officers rated needs and challenges for offenders significantly different than male officers. Policy implications of the research are discussed. This research was made possible due to a grant award from the College of Arts and Sciences at Seattle University. This research was first presented at the 2005 Academy of Criminal Justice Sciences Conference in Chicago. We are grateful to Mac Pevey and Keven Bovenkamp from the Washington State Department of Corrections and Bill Corn and Tim McTighe from United States Probation and Pretrial Services for their support, assistance, and for making the study possible. A special thanks to our research assistant Tania Reyes who was instrumental in collecting the data for this investigation.     

Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples*

Abstract: The Quantifiler^® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold (C_T) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000-fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR^® Amplification Kit to obtain interpretable short tandem repeat profiles.     

A comparison of methods used in the UK and Ireland for the extraction and detection of semen on swabs and cloth samples

The recent formation of a United Kingdom and Irish working group, the Body Fluids Forum (BFF), highlighted the need to investigate different working practices prior to any inter-laboratory comparison work and identification of best practice. Various dilutions of semen were seeded onto swabs and cloth samples for each BFF member laboratory to test using their standard techniques. The results showed that the detection of acid phosphatase on swabs is best achieved using direct testing rather than on an extract from the swab. Extraction methods for spermatozoa require a balance to be achieved between using a sufficient volume of water to ensure optimal release and minimal volume to ensure a concentrated extract. PSA tests were investigated and found to be more sensitive than Choline. DNA profiles were obtained from samples in which no spermatozoa had been detected during microscopic examination.     

This is a man’s world ... or least that’s how it looks in the journals

Mainstream criminology still tends to focus on gender as a control variable, ignoring the different pathways to crime followed by males and females. This leaves us with knowledge that we already have—males commit more criminal and delinquent acts than do females, but little information about the similarities and differences between men and women who commit crimes. On the other hand, feminist approaches tend to do one of two things: deal with girls and women only or deal with women and men separately to illuminate the differences. In this paper, we examine articles in three major journals, Criminology, Justice Quarterly, and Journal of Research in Crime and Delinquency, to explore the ways that gender is treated in mainstream criminology journals. Then, we will discuss the implications this has for feminist criminology.     

Role of Culture and Context: Ethical Issues in Research with Asian Americans and Immigrants in Intimate Violence

Ethical principles are often presented as universal, immutable rules. However, when conducting research with ethnic minority groups, such as Asian Americans and immigrants, ethical issues need to be placed within a sociocultural context as ethical responses are filtered through the specific value orientations and belief systems of the ethnic group under study. Further, when the group is a marginalized population, power dynamics complicate ethical principles of autonomy. The complexities are further accentuated with sensitive topics such as intimate violence or domestic violence. Consequently, ethical questions about autonomy, informed consent, confidentiality, limits to confidentiality, and protecting participants’ safety and reducing distress need to be at the forefront when planning intimate violence research. An overview of sociocultural context of Western biomedical ethics is presented and specific ethical issues that emerge when conducting intimate violence are discussed.