关于法庭科学DNA数据库若干问题的探讨

本文论述常染色体STR基因座的选取和数量对数据库直接匹配、亲缘关系检索、失踪人员检索等数据库检索应用可能带来的影响,指出大容量DNA数据库在应用中可能出现的问题,并讨论应用数据库时需要考虑的群体和群体内差异,以及Y染色体和线粒体DNA对数据库检索的辅助作用。旨在为今后建设和完善具有中国特色的大容量DNA数据库提供参考和帮助。     

The medical staff structure--its role in the 21st century

Dr. Eisenberg's paper presents a vision of the medical staff from the point of view of a practicing physician and medical staff leader. Dr. Eisenberg focuses on ways the medical staff, as an independent entity, may use the collective clinical knowledge and experience of its physician membership to enhance quality. This paper also presents Dr. Eisenberg's unique insights regarding the interplay and conflict between hospitals and their associated medical staff in today's complex health care delivery system. He provides several suggestions to increase cooperation between these two important components of inpatient care.     

Parents’ Communication with Adolescents About Sexual Behavior: A Missed Opportunity for Prevention?

Parents may wait to talk to their teens about sexuality until they believe their child is in a romantic relationship. To examine this, telephone surveys were conducted with 1069 parents of adolescents. Measures assessed parents’ perception of teens’ romantic involvement and parent-child communication about several sexuality topics. Multivariable regression models determined the odds of talking about each topic among parents who reported their teen had been in a romantic relationship compared to those who did not. Most parents reported talking at least a moderate amount about some sex-related topic. Parents who believed their teen had been romantically involved were more likely to have discussed most of the topics examined here (ORs=1.64 – 2.56). For some topics, associations were more pronounced among parents of younger teens. Findings suggest that parents may miss important opportunities to influence behavior, and should initiate conversations about sexuality before they believe their child to be romantically involved.Assistant Professor at the University of Minnesota. She received her Sc.D. in Social Epidemiology from the Harvard School of Public Health. Her major research interests are the social influences on high risk health behaviors among adolescents.Associate Professor with the School of Nursing and the Department of Pediatrics at the University of Minnesota. She received her Ph.D. in Epidemiology from the University of Minnesota. Her research focuses on on family, peer, and individual-level influences on adolescents’ sexual behaviors and violence involvement. She is also Deputy Director of the Healthy Youth Development Prevention Research Center, which conducts research and disseminates actionable knowledge that promotes healthy youth development and reduces health disparities among young people.Professor and Director of the Center for Adolescent Nursing at the University of Minnesota School of Nursing. She received her Ph.D. in Educational Psychology from the University of Minnesota. Her major research interests focus on understanding key risk and protective factors in adolescence, particularly among vulnerable populations of youth.President of Midwestern Professional Educational and Research Services, Inc., a DHHS funded non-profit agency serving as a Title X Regional Training Center. She received her Masters in Psychology from Miami University and worked for 12 years in flight psychophysiology before moving into public health. Her primary research interests center on identifying barriers that limit adolescent and low income population’s access to publicly funded reproductive health care services.Professor and Director of the Healthy Youth Development Prevention Research Center at the University of Minnesota Department of Pediatrics. He received his Ph.D. in Health Services Research and Policy from the University of Minnesota. His major research interests are understanding risk and protective factors in the lives of young people, particularly around issues of reproductive health, pregnancy, and violence.     

Assessing a novel room temperature DNA storage medium for forensic biological samples

The ability to properly collect, analyze and preserve biological stains is important to preserving the integrity of forensic evidence. Stabilization of intact biological evidence in cells and the DNA extracts from them is particularly important since testing is generally not performed immediately following collection. Furthermore, retesting of stored DNA samples may be needed in casework for replicate testing, confirmation of results, and to accommodate future testing with new technologies.A novel room temperature DNA storage medium, SampleMatrix™ (SM; Biomatrica, Inc., San Diego, CA), was evaluated for stabilizing and protecting samples. Human genomic DNA samples at varying amounts (0.0625-200 ng) were stored dry in SM for 1 day to 1 year under varying conditions that included a typical ambient laboratory environment and also through successive freeze-thaw cycles (3 cycles). In addition, spiking of 1-4× SM into samples prior to analysis was performed to determine any inhibitory effects of SM. Quantification of recovered DNA following storage was determined by quantitative PCR or by agarose gel electrophoresis, and evaluation of quantitative peak height results from multiplex short tandem repeat (STR) analyses were performed to assess the efficacy of SM for preserving DNA.Results indicate no substantial differences between the quality of samples stored frozen in liquid and those samples maintained dry at ambient temperatures protected in SM. For long-term storage and the storage of low concentration samples, SM provided a significant advantage over freezer storage through higher DNA recovery. No detectable inhibition of amplification was observed at the recommended SM concentration and complete profiles were obtained from genomic DNA samples even in the presence of higher than recommended concentrations of the SM storage medium. The ability to stabilize and protect DNA from degradation at ambient temperatures for extended time periods could have tremendous impact in simplifying and improving sample storage conditions and requirements. The current work focuses on forensics analysis; however this technology is applicable to all endeavors requiring storage of DNA.