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41.
This paper is focused on the preparation of samples for laser microdissection (LM) in forensic casework. In forensic genetics, it is essential to preserve and separate cellular traces during sample preparation, as they are usually gathered in very small amounts and are often contaminated with undesired cells. This is made possible by laser microdissection, a technique developed to cut cells or tissue of a certain type from a microscopical specimen by UV laser and catapult them directly into a PCR reactor. This method minimizes the risk of getting inconclusive, mixed DNA profiles due to contamination by foreign DNA and also supplies information about the cellular origin of a DNA profile. A method for optimized fixation and staining of spermatozoa for laser microdissection was established. Four different fixation methods combined with two staining methods were tested on two different microscope slides. Moreover, the effect of a blocker pen to contain the specimen on the slide was investigated.  相似文献   
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Issue importance mediates the impact of public policy issues on electoral decisions. Individuals who consider that an issue is important are more likely to rely on their attitudes toward that issue when evaluating candidates and deciding for whom to vote. The logic behind the link between issue importance and issue voting should translate to a link between issue importance and performance voting. Incumbent performance evaluations regarding an issue should have a stronger impact on the vote choice of individuals who find that issue important. The analysis demonstrates that there is a significant interaction between performance evaluations and issue importance. People concerned about an issue assign more weight to their evaluations of the government's performance on that issue when making up their mind.  相似文献   
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Dominance in the peer group is important for adolescents. Resource Control Theory posits that both coercive and prosocial (positively assertive) strategies are associated with dominance. Combining Resource Control Theory with Socioanalytic Theory on personality, we hypothesized that inspiring group members would be an additional effective strategy. This study examined whether the three behavioral strategies and two types of social skills (social competence and manipulation) predicted dominance (resource control and popularity). Participants were 619 Dutch adolescents (Mage?=?13.1; 47% female) in the first grade of secondary school. They completed peer reports (behavioral strategies and dominance) and self-reports (social skills). Only inspirational and coercive strategies substantially predicted dominance. Main effects of social skills emerged. Moderation between strategies and social skills was only observed for girls (e.g., coercive strategy use was associated with more popularity for girls with higher levels of social manipulation skills). This study furthered our understanding of the predictors of dominance in adolescence by including inspirational behavior and examining prosocial and antisocial skills.  相似文献   
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The discipline of international relations (IR) is witnessing a "constructivist turn." In this article, we argue that the new preoccupation with constructivism provides a unique opportunity to further understanding between feminism and the IR mainstream. Feminism and constructivism share a commitment to an ontology of becoming that can serve as a common basis for conversation. Yet there are also profound differences between feminists and constructivists. First, most IR feminists approach gender and power as integral elements in processes of construction, whereas most constructivists consider power to be external to such processes. This failure to conceptualize power and gender as social and pervasive leads constructivists to miss an important part of the empirical reality of power politics. Second, constructivists tend to ignore the implications of a postpositivist epistemology, whereas for feminists the question of "Who knows?" is crucial. We argue that the constructivist failure to problematize the research process as a social (and therefore political) process of construction is logically inconsistent with an ontology of becoming. We introduce empirical materials to illustrate the advantages of feminist approaches. We hope to advance a dialogue between feminism and constructivism because the two approaches add to each other and in combination can yield better theoretical and empirical understandings of the world.  相似文献   
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Die zugrundeliegende Disziplinarstrafbestimmung ist hinreichend bestimmt. Eine demokratische Gesellschaft kann die inkriminierte Werbung hinnehmen, ohne dass der Schutz des guten Rufes oder das Ansehen und die Unparteilichkeit der Rsp Schaden erleiden. Die Disziplinarstrafe verletzt die Bf im Grundrecht auf Meinungs?u?erungsfreiheit, indem die bel Beh dem Gesetz einen verfassungswidrigen Inhalt unterstellt hat.  相似文献   
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In Germany, community-based climate-protective decisions were measured on micro up to macro levels, and the role justice appraisals play in decision-making was investigated in comparison to responsibility-related and self-centered motives. Results reveal that in the field of carbon dioxide emission, gaps between those who are gaining profits and those who have to suffer from negative side effects are recognized by the community on a national as well as an international level. For gaining behavioral impact, the perception of these gaps needs to be regarded under a moral perspective and considered unjust. These justice appraisals are shown to be stable predictors of climate-protective commitments, even when powerful responsibility-related variables were included in the regression analyses. Self-centered motives proved to be of minor importance.  相似文献   
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The article surveys action taken by the European Community to combat fraud affecting its financial interests, focusing on the development of investigative authority granted to OLAF, the European‘Office Pour La Lutte Anti Fraude’ and its impact on the procedural rights of the alleged defrauder. It shows that the involvement of OLAF can be crucial for a national fraud investigation and subsequent criminal prosecution and that it meets the criteria set out by the Strasbourg organs for the applicability of Article 6 ECH. The article explores whether the legal sources governing the activities of OLAF or national—or rather, Community—law guarantee sufficient protection for the alleged defrauder and thus pay respect to principles arising from the rule of law in law enforcement. It is shown that general principles of Community law, which were mostly established in antitrust law, may provide a certain protection for the suspect, but may not protect him in all regards. It is thus argued that, in the long run, it will be necessary to provide special fair‐trial rights which offer protection to alleged defrauders from those infringements arising out of the specific features of a Community investigation.  相似文献   
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