对立法保护军人权利的思考

当前形势下,加快军人权利保护的立法步伐具有十分重要的现实意义。解决影响军人权利保护的突出问题,必须与时俱进,树立正确的权利观,把贯彻物质利益原则作为基本遵循,不断健全和规范促进法规落实的有效机制。     

体外抗草鱼呼肠孤病毒药物筛选细胞模型的建立

以草鱼肾细胞系(CIK)和草鱼呼肠孤病毒为材料,采用噻唑蓝比色法分先给药后感染、先感染后给药、药物与病毒共孵育后感染3组进行抗草鱼呼肠孤病毒药物的筛选,建立了体外抗草鱼呼肠孤病毒药物筛选的细胞模型。先给药后感染试验组的结果表明,穿琥宁在阻断草鱼呼肠孤病毒吸附和侵入细胞时效果最强,其次是利巴韦林和板蓝根,黄芩的阻断效果最弱;而在先感染后给药试验组中,穿琥宁、利巴韦林和黄芩抑制草鱼呼肠孤病毒增殖的作用显著,板蓝根抑制病毒增殖的作用不明显;在药物与病毒共孵育后感染试验组中,利巴韦林对草鱼呼肠孤病毒有一定的直接杀灭作用,黄芩、板蓝根和穿琥宁等的体外杀病毒效果不明显。该模型的建立确认了药物在CIK细胞上具有抑制草鱼呼肠孤病毒的作用,不同给药方式药物的作用效果不同,也为抗病毒药物的筛选提供了有效的技术平台。     

‘The Synthesis is in the Machine’: An Interview with Silvia Federici

ABSTRACT

In this interview Marxist feminist theorist Silvia Federici discusses the following: the relationships between accumulation and reproduction; biotechnology; the recent resurgence of social reproduction theory as exemplified by work in Endnotes and Lies; the mystification of gendered labour; the disciplining of productive bodies; the sites and technologies of primitive accumulation in the present; and the reproduction of feminism and other social movements in the twenty-first century.     

猪PD-1分子及其配体的实时荧光定量RT-PCR检测方法的建立

根据猪程序性死亡因子PD-I及其配体PD-LI和PD-L2的基因序列,分别设计了特异性引物和TaqMan探针,以10倍系列稀释的重组质粒pMD-PD-1、pMD-PD-L1和pMD-PD-L2为标准品,对实时荧光定量PCR的条件进行优化,以建立定量检测这3个基因的方法.结果表明,建立的方法在1×10~2～1×10~8copies/μL模板范围内具有良好的线性关系(r~2>0.99),扩增效率均高于96%,可检测至少100 copies的阳性标准品.利用该方法对猪外周血单核细胞中这3个基因的表达情况进行了检测,结果也表明该方法具有良好的敏感性、特异性和重复性,可应用于临床样品的检测.     

猪IFN-γ mRNA TaqMan荧光定量RT-PCR检测方法的建立

为建立一种检测猪γ-干扰素(IFN-γ)的荧光定量RT-PCR方法,针对猪IFN-γ基因和管家基因cyclophilin A(CyPA)的核苷酸序列分别设计了特异性引物和TaqMan荧光探针,以重组质粒pMD18-T-IFN-γ和pMD18-T-CyPA为标准品,进行实时荧光定量RT-PCR检测,并构建猪IFN一γ mRNA和CyPA的荧光定量RT-PCR标准曲线.结果表明,建立的方法在1×101～1×107copies/μL模板范围内具有良好的线性关系,相关系数r2均高达0.999,扩增效率均高于99.0%;可检测至少为100 copies的阳性标准品.该方法具有快速、敏感性高、特异性强和重复性好等特点,可应用于临床样品的检测.     

侦查讯问程序

作为案件侦查中的手段之一,侦查讯问程序被世界各国规定在刑事诉讼法中,而通过侦查讯问获得的证据——犯罪嫌疑人的供述和辩解即口供,亦成为诉讼中重要的证据种类。侦查讯问程序对于实现刑事诉讼查明案件真相、发现实体真实的目的具有重要的作用。文章结合国外有关侦查讯问程序的探讨,探究侦查讯问程序在诉讼运作过程中存在程序违法问题的原因,进而对侦查讯问程序进行重构及完善。     

信用证诈骗罪的规范分析

我国对于信用证诈骗犯罪的刑法规制主要体现为罪名的设置,即信用证诈骗罪(涉及《中华人民共和国刑法》第195条和第200条)。要判断这种刑法规制是否合理,是否足以遏制信用证诈骗犯罪,就需要对该罪名的概念和犯罪构成进行分析,通过规范分析查找问题与漏洞。     

Prodrugs of New Psychoactive Substances (NPS): A New Challenge

The concept of a substance acting as a prodrug for an intended drug is not new and has been known and utilized with particular benefits within medicine for efficacy and patient safety. Prodrugs of psychoactive substances are also not particularly new but this has also extended to considerations of prodrugs of new psychoactive substances (NPS). The continuing evolution of NPS has been a constant forensic challenge. In some countries, this constant evolution has led to the introduction of various alternative methods of drug control. Whether for this reason or in the pursuit of user experimentation, prodrugs of NPS have been discussed, developed, and exploited, posing some distinct forensic challenges. This is especially the case within toxicological analysis of biological fluids and for some substances, also forensic chemical analysis, through inherent instability of the prodrug or metabolism in the body. Particular examples of NPS prodrugs include 1-propanoyl-LSD, 1-butanoyl-LSD, 1-acetyl-LSD, and 2C-B-AN. This is in addition to associated substances and medicines that may be used for an intended pharmacological effect. Various prodrugs for stimulant and hallucinogenic substances in particular have appeared in the literature and have been discussed within drug user forums and made available for purchase online. Presently, drug monitoring data from national and international systems indicate that prodrugs are not widely available or problematic. Nevertheless, it is important that there is sufficient awareness of the prodrug concept and potential impact and associated forensic implications, not just for chemical analysis but also for toxicological considerations when a substance has been used.     

Moves Afoot to Subdue Smog

正SMOG has been the bane of Chinese people’s lives in recent years.But as Premier Li Keqiang recently announced to the nation and the entire international community,China is to declare war on pollution.Although fraught     

中国死刑的未来发展方向

当今社会越来越看重人道主义对现实生活的影响,国际上仍然有很多国家对我国的死刑制度持有质疑与偏见,因此我们必须从社会发展现状中了解死刑的发展,推断出中国死刑的未来发展方向,从而为应对国际舆论压力做好积极准备。