Evaluation of a Freezer Mill for Bone Pulverization prior to DNA Extraction: An Improved Workflow for STR Analysis

Traditional methods for bone pulverization typically generate heat, risking stability of DNA sample. SPEX? has developed cryogenic grinders which introduce liquid nitrogen to cool the sample and aid in the grinding process. In this study, the Freezer Mill 6970 EFM was used with two DNA extraction methods and routine downstream STR analysis procedures. DNA from as little as 0.1 g of bone powder was used to develop full STR profiles after freezer mill pulverization, and the method was reproducible. Further, no contamination was detected upon cleaning/reuse of the sample vials. There were no significant differences in DNA yield, STR alleles detected, or peak heights using the freezer mill as compared to traditional grinding, and successful DNA profiles were achieved from as low as 0.1 g of bone powder with this method. Overall, this work indicates that this cryogenic mill method may be used as a viable alternative to traditional tissue grinders.     

Evaluation of the use of pharmacological treatment with prisoners experiencing high levels of hypersexual disorder

This paper presents an evaluation of the impact of pharmacological treatment in reducing hypersexual disorder in adult males who have been incarcerated following conviction for a sexual offence. The evaluation compares two types of pharmacological treatment, one of which is part of the current NICE guidance for treatment of hypersexuality (Antiandrogens), whilst the other type (SSRIs) is off-label use in the UK for hypersexuality. The participant pool comprised 127 adult male prisoners serving sentences for sexual offences in a UK prison. Participants had been voluntarily referred for pharmacological treatment to manage hypersexual disorder. The results demonstrated a significant reduction of hypersexual disorder pre- and post-medication and contribute to the evidence base for the use of pharmacological treatment with individuals for whom hypersexual disorder may be a salient factor in their offending. Limitations of the current research are discussed.     

Equality and Regional Finance in the Americas

This article explores competing definitions of equality embedded in contending visions for regional finance in the Americas. The U.S. free market–oriented project envisions extension of a NAFTA‐like regulatory framework hemispherewide, promising Latin Americans better financial services, credit, and investment in exchange for strong financial property protections and (implicitly) dramatically reduced financial policy autonomy for their governments. Venezuela's vision of “Bolivarian” finance, exported to the Caribbean and the upper Andes, promotes assertive state management of both foreign and domestic investors, populist redistribution, and increasing reliance on nonmarket financial transactions. Brazil's regional financial project would unite South America through continentwide physical infrastructure and capitalist financial markets while retaining a role for public sector banks responsive to central government priorities. Brazil's approach shares with Venezuela's an emphasis on governments' need for financial policy authority and with the U.S. approach a concern for regulatory predictability and financial deepening.     

Book reviews

The Animal Rights/Environmental Ethics Debate ‐ The Environmental Perspective Edited by Eugene C. Hargrove. Albany, New York: State University of New York Press, 1992. Pp.xxvi, 261. Index. $19.95.     

Underlying Dimensions of DSM-5 Posttraumatic Stress Disorder and Major Depressive Disorder Symptoms

This study examined the relationship between the underlying latent factors of major depression symptoms and DSM-5 posttraumatic stress disorder (PTSD) symptoms (American Psychiatric Association, 2013). A nonclinical sample of 266 participants with a trauma history participated in the study. Confirmatory factor analyses were conducted to evaluate the fit of the DSM-5 PTSD model and dysphoria model, as well as a depression model comprised of somatic and nonsomatic factors. The DSM-5 PTSD model demonstrated somewhat better fit over the dysphoria model. Wald tests indicated that PTSD’s negative alterations in cognitions and mood factor was more strongly related to depression’s nonsomatic factor than its somatic factor. This study furthers a nascent line of research examining the relationship between PTSD and depression factors in order to better understand the nature of the high comorbidity rates between the two disorders. Moreover, this study provides an initial analysis of the new DSM-5 diagnostic criteria for PTSD.     

Environmental protection and US–Asia relations: a policy of disconnect

Despite the post-September 11 focus on regional security and the continued emphasis on regional economic cooperation, environmental degradation should not be overlooked as an important issue for US policy in and relationship with the Asia-Pacific. It is an important issue in its own right, presenting the countries of the region with ecological, economic and social (human security) challenges. There are both ethical and instrumental impulses for the United States, as a rich indus­trialised country and as a disproportionate consumer of resources and polluter of global waste, to provide environmental assistance to the Asia-Pacific. Despite global demands that the ‘new’ new world (environmental) order should be based on solidarity and collective responsibility, neither US environmental policy towards the region nor the regional consequences of its international environmental policy more generally meet this test. The US is fundamentally self-regarding rather than other-regarding in the various dimensions of its environmental relationship with the region. The consequences for both the region and for the US may be substantial. Continued environmental degradation in the region has the potential to undermine other US policy goals, in terms of its reputation, it economic objectives and even its more orthodox geopolitical security objectives.     

Visibly Invisible: EU Engagement in Conflict Resolution in the South Caucasus

Abstract

This essay examines growing European Union (EU) involvement in the South Caucasus, focusing on efforts to resolve the protracted conflicts in the regions of Abkhazia, South Ossetia and Nagorno-Karabakh. To date, the EU has occupied a back seat in conflict resolution efforts, supporting organisations such as the UN and OSCE, which have taken the lead role. However, over a decade of negotiations has produced few tangible results and the EU now has the opportunity to play a much greater role. This essay argues that the EU needs to become more involved: it has a much wider range of tools at its disposal with which to influence the various situations and it is in its own interest to ensure the stability of its neighbours.     

The Spatial Distribution of Postblast RDX Residue: Forensic Implications

Locating exactly where trace explosive residue samples should be sought during sample collection at bomb scenes is not specified in the published literature or guidelines; in this area, it is generally acknowledged that forensic practices are based on tradition rather than evidence. This study investigated patterns in the spatial distribution of postblast 1,3,5‐trinitro‐1,3,5‐triazocyclohexane residue from a series of unconfined detonations, over a range of sampling sites, and at two different detonation heights. The amount of residue recovered from the sites decreased as a function of distance from the center of the explosion. [Correction added after online publication 27 December 2012: In the preceding sentence, “increased” was corrected to “decreased” to agree with the conclusion of the article.] As the height of the detonations increased, more residues were found from all sampling sites. The findings of this empirical study have a number of important practical implications including determining where residue samples are best sought at crime scenes.