Investigation of the short-time autolysis of rat hearts by means of SDS. Polyacrylamide gel electrophoresis and electron microscopy.

The short-time autolysis of hearts was regarded as a model of ischaemic heart failure. Therefore, isolated rat hearts were subjected to 30--120 min autolysis in a Locke solution at 37 degrees C. Electron microscopic examinations and myofibrillar preparations were made from the autolysed heart ventricles. The myofibrillar proteins were resolved by SDS-polyacrylamide gel electrophoresis. After 30 min autolysis the amount of a protein of 192,000 daltons greatly increased. At the same time on the electron micrographs the focal destruction of filament destruction on the A filament area and the mitochondrial structure altered too. After 60 min autolysis another protein of 36,400 daltons appeared. On the electron micrographs the focal desintegration of Z membranes and the focal destruction of I filaments can be observed. After 120 min autolysis further proteolytic products could not be detected by gel electrophoresis but on the electron micrographs the destruction of Z membranes and I filaments became more pronounced.     

Systems analysis and emergency command and control systems for municipalities and law enforcement

This article is designed to provide insight into methodologies that can be used to assess alternative emergency command and control systems for municipalities and law enforcement. Some potential pitfalls and errors are highlighted.     

New findings concerning the pathogenesis of acute carbon monoxide (CO) poisoning

The pathomechanism of carbon monoxide (CO) poisoning was studied by means of cardiopathological and neuropathological investigations in experimental CO intoxication. It has been shown that besides CO-hemoglobin association, the binding of CO to cytochromes is a significant factor. The latter is thought to be responsible for the cytotoxic phenomena. Combined ultrastructural and cytochemical studies have enabled differentiation between toxic, hypoxic, and mixed alternations.     

Expenditures and votes: In search of downward-sloping curves in the United States and Great Britain

An earlier paper showed a negative relation between increases and accelerations in F, the ratio of federal expenditures to GNP and E, the reelection or defeat of the incumbent party in the White House over the last 100 years. This paper argues that there also exists a negative relation between V, the percentage of the popular vote cast for the incumbents, and F. This function is displaced by wars and depressions and can drift over time, making it difficult to discover negative curves in the data. Dividing British and US data for the last 50 years into party periods yields clearly discernable negative functions for F and V for the incumbents.