A hybrid ampholyte focusing technique for esterase D subtyping of evidentiary material

An ultrathin-layer polyacrylamide gel isoelectric focusing technique that uses a composite of ampholytes from three commercial sources is described for subtyping esterase D. All common allelic products of esterase D were separated clearly. The technique described in this paper provides a higher conclusive call rate on known blood specimens (95.8%) and questioned bloodstains (69.7%) compared with continuous zone electrophoresis in agarose gels (89.9 and 37.6%, respectively).     

Public policy analysis in Washington and Ottawa

Policy analysis in the U.S. and Canada tends to follow the methods of systems analysis and takes into account variables relating to substantive matters as well as political and administrative constraints that governments face in pursuing different alternatives.Comparative analysis of the political effectiveness of the two governments in implementing the results of analysis indicates that Canada has built-in constitutional advantages. Comparative assessment of bureaucratic skills in policy analysis and policy implementation suggests that the American system is relatively weaker in terms of implementation, but relatively stronger in terms of policy analytical skills. This is followed by an assessment of the attempts made by the U.S. government to improve its policymaking structures through the setting up of the Domestic Council and the reorganized OMB, and the Canadian attempts to improve its policymaking capabilities through a reorganized Cabinet committee system and the strengthening of the Privy Council Office.The final section suggests that analysis of values and norms should be an integral part of policy analysis. The scope of policy analysis should be extended to include long-term economic and political analyses.     

Informed consent issues in the cardiac transplantation evaluation

As consultation-liaison psychiatrists to a cardiac transplantation team, we have observed various informed consent issues that are particularly associated with evaluation for cardiac transplantation. We discuss complicating factors that relate to each component of informed consent and present the defense mechanism of denial as a barrier to making the decision to accept or refuse transplantation. Changes in the evaluation protocol could preclude existing impediments to provision of information and patient autonomy; however, certain intrapsychic issues must be recognized as ongoing clinical realities to be addressed as the doctrine of informed consent continues to evolve.     

The examination of glass particles using the interference objective. Part 2: A survey of flat and curved surfaces

The microscopical examination of original surfaces on casework sized glass particles using an interference objective and the interpretation of their interferograms is discussed. To reduce subjectivity, a numerical criterion was devised to assess the curvature of glass particles and this was then applied to a survey of window, container, tableware and vehicle glasses. Objects with curved surfaces produced particles that were distinctly curved while those from surfaces of both modern and old windows were clearly identifiable as flat. Apparently flat surfaces from tableware and containers almost always yielded specimens that were detectably curved. In addition, there was no evidence that a fracture surface would be confused with an original surface from a plain flat glass.In a ‘blind’ trial to assess the interferometric routines, three examiners correctly identified flat and curved surfaces on 15 particles derived from a selection of glass objects.     

Self Reports of Violence

Self-report data are used to compare the relative incidence of aggression and violence among ex-mental patients, ex-offenders, and the general population and to examine the effect of socio-demographic variables on the frequency of these behaviors. Frequency was measured by reported frequencies of aggressive behaviors during the preceding year, by whether respondents could recall serious disputes, and by the recency of the disputes they recalled. In support of studies that have relied on official arrest statistics, the evidence suggests that ex-offenders engage in violence with greater frequency than the other two groups and that they have a greater tendency to physically attack and injure their antagonists when involved in violent disputes. Ex-mental patients appear to use weapons more frequently than the general population, but they are no more likely to injure antagonists. In contrast to labeling theory, police were no more likely to become involved or to make an arrest in incidents involving ex-patients or ex-offenders.     

Expert opinion on biologic stains. Determination of status, future trends

In this paper an attempt is made to critically review the literature, with special emphasis on bloodstain analysis. One essential aim is the integration of this field into casework. Three basic components in skillful assessment of stains are described: (1) analysis of stain morphology, (2) discriminating and attributing analyses, (3) individualization. Regarding the first, the analysis of stain morphology is based upon the extensive experimental literature published since 1895--mainly in continental Europe. Since 1971 there have also been publications in the American literature. The large family of stain forms and their dependency on multiple variables are described, especially regarding the modes of formation, the energy of impact, and the physical properties of the substrate. The essential elements for reconstruction of the crime are described. The areas of application are arranged in case groups. Since in case work the stain pattern is complicated by many artifacts and overlaps, forensic pathologists are considered the ideal experts for the analysis of bloodstain patterns, as they have a profound knowledge of the type and sequence of injuries. If this is not the case, the forensic pathologist should at least be integrated into the investigating team. In practical application, the stain form is not always adequately analysed. The education and training of pathologists should be improved to achieve this standard. Analysis of the stain morphology and a subsequent selection of stains are also essential prerequisites for meaningful further investigations. By the use of discriminating and attributing analyses, one can as a rule arrive at a definite answer by using only one test. This is true for basic questions such as the identification of blood type, as well as proof of exclusion. One can distinguish between traditional methods, the new field of immunochemistry and rarely used methods. Immunochemistry has permitted success in recent years in determination of the blood group from hair. It is recommended that reference laboratories be established for training in these rare methods. Individualization analyses are subdivided into two large fields: non-DNA individualization and DNA individualization. It is postulated that in the future stain laboratory both areas will coexist. In non-DNA individualization, essential progress has been made. The detection of protein polymorphisms by blotting and subsequent visualization by antibody-linked enzyme/substrate reactions has led to a considerable increase in sensitivity and specificity.(ABSTRACT TRUNCATED AT 400 WORDS)