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31.
Use of Embryos Extracted from Individual Cannabis sativa Seeds for Genetic Studies and Forensic Applications 下载免费PDF全文
Salvador Soler Ph.D. Dionís Borràs B.Sc. Santiago Vilanova Ph.D. Alicia Sifres Ph.D. Isabel Andújar Ph.D. Maria R. Figàs M.Sc. Ernesto R. Llosa B.Sc. Jaime Prohens Ph.D. 《Journal of forensic sciences》2016,61(2):494-500
Legal limits on the psychoactive tetrahydrocannabinol (THC) content in Cannabis sativa plants have complicated genetic and forensic studies in this species. However, Cannabis seeds present very low THC levels. We developed a method for embryo extraction from seeds and an improved protocol for DNA extraction and tested this method in four hemp and six marijuana varieties. This embryo extraction method enabled the recovery of diploid embryos from individual seeds. An improved DNA extraction protocol (CTAB3) was used to obtain DNA from individual embryos at a concentration and quality similar to DNA extracted from leaves. DNA extracted from embryos was used for SSR molecular characterization in individuals from the 10 varieties. A unique molecular profile for each individual was obtained, and a clear differentiation between hemp and marijuana varieties was observed. The combined embryo extraction–DNA extraction methodology and the new highly polymorphic SSR markers facilitate genetic and forensic studies in Cannabis. 相似文献
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Legal process outsourcing (LPO) refers to the contracting of legal work from regions where it is costly to perform, such as the US to areas where it can be performed at a significantly decreased cost. LPO has been made possible by the disaggregation of the legal processes into discrete units, each of which can then outsourced to cheaper service providers. Anecdotal evidence suggests a variety of benefits such as financial gains, opportunities to perform “global” work in a corporate atmosphere and acquisition of important skills and training that enhances the prestige of the host country lawyers. In India, which has played a significant role, LPO firms are viewed as important catalysts in the transformation of the country’s highly stratified legal profession based on social identities. This qualitative study, based on 38 interviews, concludes that the corporate culture was an attractive proposition for lawyers from non-elite backgrounds; however, the commodification of offshored work led to a deprofessionalisation of lawyers, reducing them to “glorified clerks.” As a result, LPO firms only provided parallel avenues for career mobility but did not destabilise the local legal market which at its core remains socially networked. 相似文献
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Using qualitative research methods and comparing two Indian states (Gujarat and West Bengal), the authors examine the relative power and influence of trade unions in three parts of the public sector, viz., state government employment, municipal bus and tram services, and telecommunications. The distinctive methodology and the findings emphasise the importance of caution in generalising about trade unionism and industrial relations in India as a whole; and indicate that the context of particular Indian states is a vital ingredient of trade union analysis. The authors identify a complex web of explanatory factors for the relative power and influence of the unions under study, within the distinctive political, economic and historical contexts of Gujarat and West Bengal. Thus, they demonstrate the largely untapped potential for primary, qualitative and comparative research to expand our understanding of industrial relations in India. 相似文献
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Ernesto U. Savona Barbara Vettori 《European Journal on Criminal Policy and Research》2009,15(4):379-393
Whilst any estimation of crime costs is a challenge even at a national level and in respect to crimes not particularly problematic
from the definitional viewpoint, like volume crimes, the task is much harder when one has to deal with the harm caused by
organised crime, especially from a comparative perspective. First, notwithstanding many international acts and studies, the
term ‘organised crime’ is still one of those most debated and blurred in criminology. To complicate matters further, any cross-country
assessment encounters such a wide variety of national differences (cultural, in the definition of offences, and in crime data
collection systems) that the results are hardly comparable. Though extremely difficult, discussing the topic makes a great
deal of sense today, and especially within the European Union, because there is strong demand for sound knowledge on the most
harmful activities perpetrated by organised criminal groups and where these are localised. Considering the increasing importance
attached to the issue, this article critically discusses existing attempts to measure organised crime harm from a comparative
perspective, highlighting their strengths and weaknesses. It first reviews harm assessment models developed to date at the
international level, and mainly consisting of surveys. It then presents a different approach to the subject, one more centered
on official statistics and which has recently resulted in the development of a methodology in the context of a EU-funded study
entitled IKOC (Improving Knowledge on Organised Crime to develop a common European approach). 相似文献
35.
Fabio De‐Giorgio M.D. Maria Lodise M.D. Marcello Chiarotti M.D. Ernesto d'Aloja M.D. Arnaldo Carbone M.D. Luca Valerio M.D. 《Journal of forensic sciences》2013,58(5):1397-1400
Acetaminophen or paracetamol, a commonly used over‐the‐counter analgesic, is known to elicit severe adverse reactions when taken in overdose, chronically at therapeutic dosage or, sporadically, following single assumptions of a therapeutic dose. Damage patterns including liver damage and, rarely, acute tubular necrosis or a fixed drug exanthema. We present a case of fatal acetaminophen toxicity with postmortem blood concentration 78 μg/mL and unusual clinical features, including a visually striking and massive epidermolysis and rhabdomyolysis, disseminated intravascular coagulation and myocardial ischemia. This case is compared with the most similar previous reports in terms of organ damage, clinical presentation, and cause of death. We conclude that a number of severe patterns of adverse effects to acetaminophen are emerging that were previously greatly underestimated, thus questioning the adequacy of the clinical spectrum traditionally associated with acetaminophen intoxication and leading to the need to review this spectrum and the associated diagnostic criteria. 相似文献
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Ernesto Calvo Aldo F. Ponce 《Studies in Comparative International Development (SCID)》2013,48(4):331-355
In the last 15 years, considerable efforts have been directed towards understanding how currency shocks affect the clout of economic actors. Little research, however, has studied how currency shocks affect the salience of key economic actors and their organizations among the public. In this article, we test for the effect of currency shock on the media salience and legislative importance of economic actors. We use a novel empirical design that (1) measures the level of media salience of 23 Labor, Rural, Business, and Financial Associations and (2) measures the targets of legislation initiated in key Congressional committees. Results show that currency shocks have moderate effects on the media salience of economic actors and more pronounced policy consequences, reflected on the legislative importance of key economic jurisdictions in Congress. 相似文献
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Jens Großer Ernesto Reuben Agnieszka Tymula 《American journal of political science》2013,57(3):582-597
We experimentally study the common wisdom that money buys political influence. In the game, one special interest (i.e., a corporate firm) has the opportunity to influence redistributive tax policies in her favor by transferring money to two competing candidates. The success of the investment depends on whether or not the candidates are willing and able to collude on low‐tax policies that do not harm their relative chances in the elections. In the experiment, successful political influence never materializes when the firm and candidates interact just once. By contrast, it yields substantially lower redistribution in about 40% of societies with finitely repeated encounters. However, investments are not always profitable, and profit sharing between the firm and candidates depends on prominent equity norms. Our experimental results shed new light on the complex process of buying political influence in everyday politics and help explain why only relatively few firms do actually attempt to influence policymaking. 相似文献
38.
Suicidal or Homicidal Sharp Force Injuries? A Review and Critical Analysis of the Heterogeneity in the Forensic Literature 下载免费PDF全文
Fabio De-Giorgio M.D. Ph.D. Maria Lodise M.D. Gianluigi Quaranta M.D. Ph.D. Antonio G. Spagnolo M.D. Ernesto d'Aloja M.D. Ph.D. Vincenzo L. Pascali M.D. Ph.D. Vincenzo M. Grassi M.D. 《Journal of forensic sciences》2015,60(Z1):S97-S107
The differential diagnosis between self-inflicted and nonself-inflicted, suicidal and homicidal, injuries is difficult or impossible in many cases and, above all, cannot be made on the basis of information obtained solely from the autopsy or the medicolegal clinical examination. The purpose of this study is to analyze the literature on suicidal and homicidal sharp force injuries and identify the relevant parameters that may help differentiate between suicidal and homicidal deaths. To achieve this goal, a review of 595 potentially relevant articles was performed. After excluding the nonrelevant papers by screening the titles, all abstracts were reviewed, and articles meeting the inclusion criteria underwent a full-text review. The following parameters were compiled into a table: number of cases, localization of the injuries, and number of injuries. The data were statistically analyzed and compared with those available in the forensic literature. On the basis of the heterogeneity of data revealed by the present review, a simple and short checklist of the parameters that should be included when reporting suicides and homicides by sharp force has been proposed. 相似文献
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