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201.
Evaluation of a Freezer Mill for Bone Pulverization prior to DNA Extraction: An Improved Workflow for STR Analysis 下载免费PDF全文
Emely Morales Colón M.S. Mireya Hernández M.S. Mariel Candelario M.S. María Meléndez B.S. Tracey Dawson Cruz Ph.D. 《Journal of forensic sciences》2018,63(2):530-535
Traditional methods for bone pulverization typically generate heat, risking stability of DNA sample. SPEX? has developed cryogenic grinders which introduce liquid nitrogen to cool the sample and aid in the grinding process. In this study, the Freezer Mill 6970 EFM was used with two DNA extraction methods and routine downstream STR analysis procedures. DNA from as little as 0.1 g of bone powder was used to develop full STR profiles after freezer mill pulverization, and the method was reproducible. Further, no contamination was detected upon cleaning/reuse of the sample vials. There were no significant differences in DNA yield, STR alleles detected, or peak heights using the freezer mill as compared to traditional grinding, and successful DNA profiles were achieved from as low as 0.1 g of bone powder with this method. Overall, this work indicates that this cryogenic mill method may be used as a viable alternative to traditional tissue grinders. 相似文献
202.
Olalla Baz Esther Fernández-Molina 《European Journal on Criminal Policy and Research》2018,24(3):237-252
The empirical evidence on the process-based model of self-regulation shows that procedural justice evaluations and the perceived legitimacy of authorities impact law-abiding behavior. However, few studies analyze this theory from the perspective of adolescent legal socialization. The present study aims to examine the process-based model and other socializing agents such as family, school and peers that may have an effect on it. The sample comprised 2041 youths residing in Spain, aged between 13 and 18 years. The data form part of the Third International Self-Report Delinquency Study (ISRD-3). Multiple linear regression analyses were conducted to predict police legitimacy and juvenile delinquency. The results reveal that police legitimacy perceptions are not only influenced by procedural justice, but also by parental monitoring, school attachment, and delinquent peers. Moreover, perceptions of police legitimacy, parental monitoring, and delinquent peers predict juvenile delinquency. These findings complement and add new explanatory factors to the process-based model. 相似文献
203.
Luis V. Prandel Ph.D. Vander de F. Melo Ph.D. André M. Brinatti Ph.D. Sérgio da C. Saab Ph.D. Fábio A. S. Salvador Ph.D. 《Journal of forensic sciences》2018,63(1):251-257
Soil vestiges might provide information about a crime scene. The Rietveld method with X‐ray diffraction data (RM‐XRD) is a nondestructive technique that makes it possible to characterize minerals present in the soils. Soil clays from the metropolitan region of Curitiba (Brazil) were submitted to DCB treatment and analyzed using XRD with CuKα radiation in the step‐scan mode (0.02° 2θ/5 s). The GSAS+EXPGUI software was used for RM refinement. The RM‐XRD results, together with the principal component analysis (PCA) (52.6% total variance), showed the kaolinite predominance in most analyzed samples and the highest quartz contents in “site 1.” Higher anatase, and gibbsite and muscovite contents influenced discrimination, mainly in “site 3” and “site 1,” respectively. These results were enough to discriminate clays of four sites and two horizons using a reduced amount of sample showing that the technique can be applied to the investigation into soil vestiges. 相似文献
204.
Václav Janeček 《Computer Law & Security Report》2018,34(5):1039-1052
This article analyses, defines, and refines the concepts of ownership and personal data to explore their compatibility in the context of EU law. It critically examines the traditional dividing line between personal and non-personal data and argues for a strict conceptual separation of personal data from personal information. The article also considers whether, and to what extent, the concept of ownership can be applied to personal data in the context of the Internet of Things (IoT). This consideration is framed around two main approaches shaping all ownership theories: a bottom-up and top-down approach. Via these dual lenses, the article reviews existing debates relating to four elements supporting introduction of ownership of personal data, namely the elements of control, protection, valuation, and allocation of personal data. It then explores the explanatory advantages and disadvantages of the two approaches in relation to each of these elements as well as to ownership of personal data in IoT at large. Lastly, this article outlines a revised approach to ownership of personal data in IoT that may serve as a blueprint for future work in this area and inform regulatory and policy debates. 相似文献
205.
Rubén González‐Vicente 《拉美政治与社会》2013,55(1):46-72
This article analyzes the developmental impact of two of the earliest investments made by Chinese companies in South America, the Shougang Corporation's mining activities in Peru and Andes Petroleum's oil extraction operations in Ecuador. The article draws attention to the importance of contextualizing and disaggregating instances of Chinese resource‐based investment in order to adequately grasp the complexity of processes that are contingent to particular regimes of natural resource governance, companies' backgrounds, and the strength and nature of local reactions, among other factors. It thereby encourages a critical examination of Chinese investment in South America that explores how the characteristics of that investment are reshaped by the long and contested histories of resource extraction in the region, the promotion of and resistance to particular visions of development, the agency of multiply situated and complex actors, and the wider transnational production networks in which resource extraction processes are embedded. 相似文献
206.
After the period of hyperinflation (1989, 1990) the Argentine economy stabilized and most of public companies were privatized. The return on investments was concentrated on promoting the technological change which adopts two basic forms: systemic modernization and revamping of existing teams. The first variant -ilustrated by the national telephone network- makes obsolete the knowledge accumulated during the period when the company was public. To confront the structural deficiency of knowledge and experience which result, the private operators invest massively in transfering educational technologies and in the development of costly and sofisticated internal systems of training, while they negotiate numerous and various agreements of cooperation with educational system institutions, public as well as private. In the second variant - illustrated by a steel and metallurgic company - the preexisting knowledge continues being necessary even though it may need a vis-a-vis updating facing the new demands which are stated referring to the productivity, quality, effectiveness and productive efficiency in markets which are open to competition. Taking into account the significant achievements which are being obtained by this second alternative of modernization - most of the privatized companies fall into this pattern- the article invites to a reopening of the old debate on technological blending. 相似文献
207.
Francisco Javier Ansuátegui Roig 《Ratio juris》2013,26(1):144-148
208.
Cormac Ó Gráda 《The History of the Family》2013,18(4):350-359
The paper offers an analysis of the demography of the confessionally-divided and heavily industrialized northern Irish town of Lurgan in the early twentieth century. Its main source is the individual household forms of the Irish population census of 1911. This was the first Irish census to provide household-level data on the number of children born—and the number still surviving—to married couples co-resident at the time of the census. The paper begins by producing standard cross-tabulations of socio-economic status, as represented by literacy, numeracy, occupation, and the labour force participation of married women, by religion. It then presents econometric analyses of female labour force participation, marital fertility, and infant and child mortality. The greater poverty of Catholic households forced a higher proportion of mothers to work outside the home, mainly as unskilled workers in the town's linen mills. The analysis of the marital fertility suggests that confessional affiliation did not influence family size much at this juncture. The analysis of infant and child mortality suggests that religious affiliation was to a significant extent a cloak for living standards and socio-economic advantage. 相似文献
209.
Jamie Rosen 《Family Court Review》2013,51(2):330-343
Increasingly lawyers for children follow a model of “client centered” (as opposed to “best interests”) representation in child custody disputes in which the child client defines the objectives of the representation. The client‐centered model, while appropriate in most cases to give voice to the child's preferences in a process that deeply impacts him or her, can create an ethical dilemma for the child's lawyer in cases where a child is truly alienated from the other parent by the actions of the alienating parent. Alienated children strongly and unreasonably express a preference for objectives of representation that might further damage the alienated parent's relationship with the child. The alienated child's objectives may be the result of a campaign of denigration and “brainwashing” by the alienating parent. This Note suggests that when a child is truly alienated from a parent, as diagnosed by a mental health expert, the child may have “diminished capacity” and therefore, the client‐directed model of representation is not adequate. This Note proposes that the Child's Attorney must determine whether the child is of diminished capacity under the Model Rules of Professional Conduct and, if so, must treat the client accordingly under Rule 1.14. Specifically, the attorney may, if all other remedial measures are inadequate, override the child's wishes and advocate a position that the child would take, but for the brainwashing of the child used to alienate him or her from a parent. 相似文献
210.
David Ortiz Rodríguez Andrés Navarro Galera Antonio M. López Hernández 《International Public Management Journal》2013,16(4):371-398
ABSTRACT The usefulness of performance indicators increases when organizations compare their data. However, most discussions have failed to emphasize two lines of action that could improve the relevance of such comparisons: a) agreement among service managers on the indicators to be used in the evaluation; b) homogenization of the indicators to be used by organizations, and of the process used to calculate them. In this context, this paper proposes a methodology based on the participation of and consensus among managers, which helps to standardize the construction and presentation of performance indicators. The aim of this proposal is to improve the comparative evaluation of organizations. Finally, in order to test the proposal, a practical application is carried out on the local culture departments of nine large municipalities in southern Spain. 相似文献