Genotyping of DNA samples under adverse conditions of Low Copy Number—LCN (formolisados tissue samples and embedded in paraffin)

This paper aims to describe and evaluate a protocol for extraction of DNA (deoxyribonucleic acid) in formalinized tissues and embedded in paraffin for forensics genetic analysis. In outline the method is the removal of paraffin with an organic solvent in 0.3–0.5 mg of the sample of the tissue under study, followed by removal of formaldehyde, rehydration and soon after the extraction of genomic DNA. The extraction is achieved through the stages of cellular lysis, enzymatic digestion of proteins and DNA precipitation in ethanol medium. With the research we can conclude that even when the DNA is present in small quantities in conditions of extreme difficulties in its extraction, as formalinized tissues and embedded in paraffin, the technique of optimizing the extraction of DNA used both to organic extraction as Chelex, for use in the polymerase chain reaction (PCR), and possible the investigation of different samples of human tissue, biological samples, or was obtained under the conditions tested, a DNA with good quality and concentration. The samples were amplified for the mini-STRs loci using the product marketed in multilocus, using a methodology recommended by the supplier and validated for analysis of forensic DNA. Commercial kit was used MiniFiler from Applied Biosystems. The DNA fragments amplified by PCR showed that the extracted DNA had good amplification.     

Results of the 2008 Colombian paternity testing quality control exercise

The Reference National Laboratory, Genes Ltda, designated by the Commission of Accreditation and Alertness created by Law 721 of 2001 of Republic of Colombia, organized and coordinated the Quality Control Exercise of 2008 for laboratories undertaking paternity and maternity tests with DNA markers. The Quality Control Exercise included both practical and theoretical exercises. For the practical exercise, three blood samples in FTA Classic Card were sent to each participating laboratory to be genotyped for DNA markers using the routine methodologies in their laboratories. For the theoretical exercise, it was asked to the participating laboratories to calculate the partial and final paternity indexes based on two genetic profiles of an alleged biological father and his son. Allele frequencies were made available to the participants, as well as Y chromosome haplotype database. A total of 12 laboratories have participated with data from 57 STRs, including autosomal and sex chromosome markers. Consensus was found in 37 STRs, 21 in autosomes and 16 Y chromosome linked. The rate of reporting errors was 3.1% (concentrated in just one laboratory). The theoretical exercise had consensus.     

Financial reporting transparency,citizens' understanding,and public participation: A survey experiment study

This study investigates the conditions under which transparency contributes to citizens' understanding of financial reporting and examines how this enhanced understanding is associated with public participation. To this end, a survey experiment was conducted in which two attributes of financial reporting transparency (i.e., content clarification and presentation format) were the manipulated variables, whereas citizens' understanding and public participation were the outcome variables. Results demonstrate that the provision of explanations to clarify obscure technical jargon does have a positive effect on citizens' understanding. A similar effect was found for the provision of graphical and visual representations. However, the study reveals that there is no additional benefit in simultaneously providing both explanations of technical jargon and visual aids. Furthermore, findings show that the levels of public participation are highest among the individuals who felt they understood the financial information the best, but yet possessed the lowest level of actual understanding.     

The Political Cost-Effectiveness of Private Vessel Protection: The Italian Case

Italy has traditionally been wary of private providers of security. Still, private military and security companies (PMSCs) have recently started to play an important role in protecting Italian merchant vessels, eventually replacing the military vessel protection detachment units (VPDs) provided by the Italian Navy. Drawing on neoclassical realism, the increasing involvement of PMSCs in protecting Italian merchant ships is presented as an attempt to reduce the political costs associated with the use of military personnel abroad, epitomised by the arrest of two Italian Navy fusiliers by Indian authorities in February 2012.     

The cholera epidemic of 1833 and mortality in Mexico City

The author examines the impact of the 1833 cholera epidemic in Mexico City, Mexico, on social, economic, and political aspects of life in that city. She finds that some five percent of the population died during the epidemic, and enumerates them by age and sex.     

Evolutions and Limits of New Public Management—Inspired Budgeting Practices in Italian Local Governments

In Italy, New Public Management (NPM)‐inspired reforms have been mostly legislation‐driven. For Italian Local Governments (LGs), one of the most significant reforms was the introduction, in 1995, of an “Executive Management Plan” (EMP). The EMP has been interpreted and applied by Italian LGs differently across space and over time. Some LGs have introduced it only formally. Others have interpreted it as a tool to define individual managers's spending authorizations. Only some have viewed it as a building block of a wider management control system. Over time, LGs have since been making continuous changes to their budgeting systems, without yet reaching a new steady state.     

The UN decade for women in Peru

Peru will see the International Decade of Women come to an end with some real achievements tempered only by doubts as to their endurance and long-term impact on the conditions of life of the great majority of poor women, in cities, towns, and the rural countryside. To evaluate the likelihood of a lasting effect it is useful to review some of the actions which were taken under the stimulus of the international call to give priority to the concerns of women in public and private institutions.