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201.
202.
Becker J Schmidt P Musshoff F Fitzenreiter M Madea B 《Forensic science international》2004,140(1):13-20
The expression of the human micro-opiate receptor (MOR1) in post mortem human brain tissue was examined using real-time PCR technology. Tissue samples from 11 fatalities due to opiate overdose and five normal subjects with different causes of death were analysed in order to elucidate whether chronic opiate abuse is followed by a regulation of MOR1 expression. In each case nine selected brain regions (thalamus, caudate nucleus, hypothalamus, ventral tegmentum, hippocampus, amygdala, frontal cortex, nucleus accumbens, putamen) were evaluated. The MOR1-mRNA level was determined relative to the housekeeping gene beta2-microglobulin. While in most regions the MOR mRNA levels in the brain of addicts were not different from the control group-with varying levels between 0 and 15% of housekeeping gene level-in the brains of three drug-related fatalities an enormous increase was encountered in the thalamus where the MOR-mRNA level amounted for up to 10,000% of the measured housekeeping gene level. The results obtained by toxicological hair analysis in the group of drug-related fatalities indicate that the enormous thalamic MOR1-expression is primarily found in individuals who died from acute heroin overdose but did not show signs of a substantial chronic administration of the drug. Further studies have to be performed to evaluate if the observed MOR1-mRNA up-regulation in the thalamus in a subpopulation of acute lethal intoxications mirrors a state of functional hypersensitivity associated with the occurrence of death. 相似文献
203.
Kereszturya L Rajczya K Lászikb A Gyódia E Pénzes M Falus A Petrányia GG 《The American journal of forensic medicine and pathology》2002,23(1):57-62
In cases of disputed paternity, the scientific goal is to promote either the exclusion of a falsely accused man or the affiliation of the alleged father. Until now, in addition to anthropologic characteristics, the determination of genetic markers included human leukocyte antigen gene variants; erythrocyte antigens and serum proteins were used for that reason. Recombinant DNA techniques provided a new set of highly variable genetic markers based on DNA nucleotide sequence polymorphism. From the practical standpoint, the application of these techniques to paternity testing provides greater versatility than do conventional genetic marker systems. The use of methods to detect the polymorphism of human leukocyte antigen loci significantly increases the chance of validation of ambiguous results in paternity testing. The outcome of 2384 paternity cases investigated by serologic and/or DNA-based human leukocyte antigen typing was statistically analyzed. Different cases solved by DNA typing are presented involving cases with one or two accused men, exclusions and nonexclusions, and tests of the paternity of a deceased man. The results provide evidence for the advantage of the combined application of various techniques in forensic diagnostics and emphasizes the outstanding possibilities of DNA-based assays. Representative examples demonstrate the strength of combined techniques in paternity testing. 相似文献
204.
Immunoaffinity extraction units (LSD ImmunElute) are commercially available for the analysis of lysergic acid diethylamide (LSD) in urine. The ImmunElute resin contains immobilized monoclonal antibodies to LSD. We applied the ImmunElute procedure to serum and also to human hair samples. For hair analysis the samples were first extracted with methanol under sonication. The extracts were then purified using the ImmunElute resin. LSD analysis was carried out with HPLC and fluorescence detection. The immunoaffinity extraction provides highly purified extracts for chromatographic analysis. The limit of detection (signal-to-noise ratio = 3) has been determined to be < 50 pg regardless of which sample material was used. The procedure was applied to authentic hair samples from drug abusers (n = 11). One of these samples tested positive with an amount of 110 pg LSD in 112 mg extracted hair corresponding to a concentration of 1 pg/mg. 相似文献
205.
Testing Legal Assumptions Regarding the Effects of Dancer Nudity and Proximity to Patron on Erotic Expression 总被引:1,自引:0,他引:1
Linz D Blumenthal E Donnerstein E Kunkel D Shafer BJ Lichtenstein A 《Law and human behavior》2000,24(5):507-533
A field experiment was conducted in order to test the assumptions by the Supreme Court in Barnes v. Glen Theatre, Inc. (1991) and the Ninth Circuit Court of Appeals in Colacurcio v. City of Kent (1999) that government restrictions on dancer nudity and dancer–patron proximity do not affect the content of messages conveyed by erotic dancers. A field experiment was conducted in which dancer nudity (nude vs. partial clothing) and dancer–patron proximity (4 feet; 6 in.; 6 in. plus touch) were manipulated under controlled conditions in an adult night club. After male patrons viewed the dances, they completed questionnaires assessing affective states and reception of erotic, relational intimacy, and social messages. Contrary to the assumptions of the courts, the results showed that the content of messages conveyed by the dancers was significantly altered by restrictions placed on dancer nudity and dancer–patron proximity. These findings are interpreted in terms of social psychological responses to nudity and communication theories of nonverbal behavior. The legal implications of rejecting the assumptions made by the courts in light of the findings of this study are discussed. Finally, suggestions are made for future research. 相似文献
206.
207.
208.
Lefkowitz Eva S. Boone Tanya L. Shearer Cindy L. 《Journal of youth and adolescence》2004,33(4):339-351
During emerging adulthood, relationships with close friends may be particularly important and influential, yet most research on communication about sex-related topics focuses on emerging adults' communication with parents. Two hundred and five college students (61% female, mean age = 20.5) completed surveys about the frequency and quality of their sex-related conversations with same-sex best friends, and their sexual attitudes and behaviors. These conversations were more frequent between females, and between less religious youth. Sexually active youth discussed most topics more frequently than abstinent youth, although abstinent youth discussed abstinence more frequently. More frequent and comfortable conversations were generally associated with more liberal sexual attitudes, and more positive condom-related attitudes. Differential associations between the specific topics discussed and attitudes about sexuality, HIV, and condoms highlight the importance of examining multiple dimensions of sex-related communication rather than the unidimensional measures typically employed. 相似文献
209.
Fifty-two mother-adolescent dyads (mean adolescent age = 16.3) participated in an observational study of communication about
health topics. The aim of the study was to examine mother-adolescent conversations about health issues—drugs/alcohol, sexuality,
nutrition/exercise—to determine the extent to which the mothers treat these issues similarly. Across conversations, mothers
spent more time asking questions than lecturing or discussing negative consequences. Mothers discussed negative consequences
less in the nutrition/exercise and sexuality conversations than in the drug/alcohol conversation. Mothers asked fewer questions
when discussing nutrition/exercise than drugs/alcohol, and lectured more in the nutrition/exercise conversation than in the
sexuality conversation. The results of this study have implications for intervention programmers advocating in the media that
parents “talk to your kids” about these health issues.
Tanya L. Boone is an Assistant Professor of Psychology at California State University, Bakersfield. She received her Ph.D.
in 2003 from The Pennsylvania State University. Her major research interests include adolescent risk behaviors and the influences
on such behaviors, with a focus on family influences.
Eva S. Lefkowitz is an Associate Professor of Human Development and Family Studies at The Pennsylvania State University. She
received her Ph.D. in 1998 from the University of California, Los Angeles. Her major research interests include the development
of attitudes, behaviors, and relationships during adolescence and emerging adulthood. 相似文献
210.
The German federal governmental system is conceptualized as a full-fledged two-level system, in which the Länder governments participate in federal policy decisions via the second chamber Bundesrat and in which the stakes of state coalition building are high for the federal parties. Our research question is whether we can find systematic empirical evidence for an influence of federal on state parties to build state governments whose party composition is concordant with federal politics, containing either exclusively federal governmental or non-governmental parties. We answer this question by indirect evidence. We show that such concordant majority coalitions occur above average even if important coalition predictors are controlled as minimal winning coalitions or participation of dominant and/or central players. We predict the 182 actual Land governments which were formed in the period from 1949 to 2003 compared to the possible governments in each situation. 相似文献