Combination of DNA-based and conventional methods to detect human leukocyte antigen polymorphism and its use for paternity testing

In cases of disputed paternity, the scientific goal is to promote either the exclusion of a falsely accused man or the affiliation of the alleged father. Until now, in addition to anthropologic characteristics, the determination of genetic markers included human leukocyte antigen gene variants; erythrocyte antigens and serum proteins were used for that reason. Recombinant DNA techniques provided a new set of highly variable genetic markers based on DNA nucleotide sequence polymorphism. From the practical standpoint, the application of these techniques to paternity testing provides greater versatility than do conventional genetic marker systems. The use of methods to detect the polymorphism of human leukocyte antigen loci significantly increases the chance of validation of ambiguous results in paternity testing. The outcome of 2384 paternity cases investigated by serologic and/or DNA-based human leukocyte antigen typing was statistically analyzed. Different cases solved by DNA typing are presented involving cases with one or two accused men, exclusions and nonexclusions, and tests of the paternity of a deceased man. The results provide evidence for the advantage of the combined application of various techniques in forensic diagnostics and emphasizes the outstanding possibilities of DNA-based assays. Representative examples demonstrate the strength of combined techniques in paternity testing.     

Testing Legal Assumptions Regarding the Effects of Dancer Nudity and Proximity to Patron on Erotic Expression

A field experiment was conducted in order to test the assumptions by the Supreme Court in Barnes v. Glen Theatre, Inc. (1991) and the Ninth Circuit Court of Appeals in Colacurcio v. City of Kent (1999) that government restrictions on dancer nudity and dancer–patron proximity do not affect the content of messages conveyed by erotic dancers. A field experiment was conducted in which dancer nudity (nude vs. partial clothing) and dancer–patron proximity (4 feet; 6 in.; 6 in. plus touch) were manipulated under controlled conditions in an adult night club. After male patrons viewed the dances, they completed questionnaires assessing affective states and reception of erotic, relational intimacy, and social messages. Contrary to the assumptions of the courts, the results showed that the content of messages conveyed by the dancers was significantly altered by restrictions placed on dancer nudity and dancer–patron proximity. These findings are interpreted in terms of social psychological responses to nudity and communication theories of nonverbal behavior. The legal implications of rejecting the assumptions made by the courts in light of the findings of this study are discussed. Finally, suggestions are made for future research.     

Communication with Best Friends About Sex-Related Topics During Emerging Adulthood

During emerging adulthood, relationships with close friends may be particularly important and influential, yet most research on communication about sex-related topics focuses on emerging adults' communication with parents. Two hundred and five college students (61% female, mean age = 20.5) completed surveys about the frequency and quality of their sex-related conversations with same-sex best friends, and their sexual attitudes and behaviors. These conversations were more frequent between females, and between less religious youth. Sexually active youth discussed most topics more frequently than abstinent youth, although abstinent youth discussed abstinence more frequently. More frequent and comfortable conversations were generally associated with more liberal sexual attitudes, and more positive condom-related attitudes. Differential associations between the specific topics discussed and attitudes about sexuality, HIV, and condoms highlight the importance of examining multiple dimensions of sex-related communication rather than the unidimensional measures typically employed.     

Mother-Adolescent Health Communication: Are All Conversations Created Equally?

Fifty-two mother-adolescent dyads (mean adolescent age = 16.3) participated in an observational study of communication about health topics. The aim of the study was to examine mother-adolescent conversations about health issues—drugs/alcohol, sexuality, nutrition/exercise—to determine the extent to which the mothers treat these issues similarly. Across conversations, mothers spent more time asking questions than lecturing or discussing negative consequences. Mothers discussed negative consequences less in the nutrition/exercise and sexuality conversations than in the drug/alcohol conversation. Mothers asked fewer questions when discussing nutrition/exercise than drugs/alcohol, and lectured more in the nutrition/exercise conversation than in the sexuality conversation. The results of this study have implications for intervention programmers advocating in the media that parents “talk to your kids” about these health issues. Tanya L. Boone is an Assistant Professor of Psychology at California State University, Bakersfield. She received her Ph.D. in 2003 from The Pennsylvania State University. Her major research interests include adolescent risk behaviors and the influences on such behaviors, with a focus on family influences. Eva S. Lefkowitz is an Associate Professor of Human Development and Family Studies at The Pennsylvania State University. She received her Ph.D. in 1998 from the University of California, Los Angeles. Her major research interests include the development of attitudes, behaviors, and relationships during adolescence and emerging adulthood.     

A systematic approach to improve downstream single-cell analysis for the DEPArray™ technology

Most commercially available STR amplification kits have never been fully validated for low template DNA analysis, highlighting the need for testing different PCR kits and conditions for improving single-cell profiling. Here, current strategies rely mainly on adjusting PCR cycle number and analytical threshold settings, with a strong preference for using 30 amplification cycles and thresholds at 30–150 RFU for allele detection. This study aimed to (1) determine appropriate conditions for obtaining informative profiles utilizing a dilution series, and (2) test the outcome on single cells using the DEPArray™ technology. Four routinely applied forensic STR kits were compared by using three different amplification volumes and DNA dilutions down to 3.0 pg, while two well-performing kits were used for single/pooled leucocyte and sperm cell genotyping. Besides reduced costs, the results demonstrate that a 50%–75% PCR volume reduction was beneficial for peak height evaluation. However, this was counteracted by an increased artifact generation in diluted DNA volumes. Regarding profile completeness, the advantage of volume reduction was only prominent in samples processed with Fusion 6C. For single and pooled cells, ESIFast and NGMDetect provided a solid basis for consensus profiling regarding locus failure, although locus dropouts were generally observed as stochastic events. Amplification volume of 12.5 μL was confirmed as appropriate in terms of peak heights and stutter frequencies, with increased stutter peaks being the main artifact in single-cell profiles. Limitations associated with these analyses are discussed, providing a solid foundation for further studies on low template DNA.     

Investing in international security: rising powers and organizational choices

How do rising powers choose to allocate their finite resources among the multiple global and regional security organizations? Building on the literatures on forum shopping and rising powers, we argue that the different organizational investment choices of rising powers are explained by varying regional ideational affinities. Organizational settings have ideational foundations that can look very different from region to region. We argue that regional ideational affinity leads rising powers to invest in regional rather than global organizations. However, if the ideational composition of the region is highly diverse, global organizations are a better vehicle to accommodate rising powers’ emergent ambitions. To demonstrate our argument, we examine the choices of Brazil and South Africa in terms of their material and ideational investments in regional and global organizations.     

Willingness to Cooperate with the Police in Four Central European Countries

Social order and security depend on mutual cooperation between the police and the public. Since the majority of crime is not detected by the police itself, informal control is needed to ensure order in society. This article aims to describe the circumstances under which people´s willingness to cooperate with the police is enhanced. Recent studies show that public compliance and cooperation with authorities who carry out criminal proceedings are linked with the extent to which people perceive these authorities as trustworthy and legitimate. Importantly, trust in police procedural fairness leads to the perception that institutions of justice are legitimate, which in turn enhances people´s willingness to cooperate with them in order to fight crime and disorder. This normative perspective is supported in many European countries. However, evidence exists that instrumental judgements, which focus on one´s self-interest and on outcomes of the justice system, could also be important in some countries. Drawing on procedural justice theory, we examine the importance of normative and instrumental factors in eliciting people´s readiness to help the police fight crime in four Central European countries: the Czech Republic, the Slovak Republic, Hungary, and Poland. While the procedural justice pattern, i.e. the normative perspective, holds well in the Czech Republic and Hungary, in other analysed countries trust in police effectiveness or fear of crime, i.e. instrumental judgements, are relevant too.     

Challenges in digital forensics

Abstract

Various terms have been used to describe the intersection between computing technology and violations of the law-including computer crime, electronic crime, and cybercrime. While there remains little agreement on terminology, most experts agree that the use of electronic devices to commit crime has increased dramatically and is now commonplace. It is the role of the digital investigator to bring cybercriminals to justice. Cybercrime however differs from traditional crime and presents a variety of unique challenges including the variety of electronic devices available, amount of data produced by these devices, the absence of standard practices and guidelines for analyzing that data, the lack qualified personnel to perform investigations and the lack of resources to provide on-going training. This paper examines these challenges     

Market-Oriented Urban Planning – Constraining Citizen Participation

Urban planning in Norway can be characterised as market oriented, with responsibilities for the formulation of planning largely delegated to private developers. Even though the principle of citizen participation has a strong and longstanding tradition in Norway, the market-oriented practices challenge the ability of citizens to influence their spatial surroundings. Based on broad surveys and qualitative case studies, this article maps the attitudes of developers, councillors and planners towards citizen participation and studies the strategies of local community associations. Our analysis shows that developers value citizen participation to a much lesser degree than councillors and planners, which can explain the lack of participatory channels in early phases. Official avenues for participation occur later, primarily through hearings. Local associations find this to be too late, characterising urban planning as a path dependent process; as a consequence, local associations attempt to influence planning processes in a more informal way by contacting councillors directly.