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Sánchez-Diz P Gusmão L Beleza S Benítez-Páez A Castro A García O Solla LP Geada H Martín P Martínez-Jarreta B de Fátima Pinheiro M Raimondi E Silva de la Fuente SM Vide MC Whittle MR Zarrabeitia MT Carracedo A Amorim A;GEP-ISFG 《Forensic science international》2003,135(2):158-162
A collaborative exercise was carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG) in order to evaluate the performance of two Y-chromosome STR PCR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The participating laboratories were asked to type three samples for the eight markers, using a specific amplification protocol. In addition, two control samples, with known haplotypes, were provided. The results obtained by the 13 different participating laboratories were identical, except for two laboratories that failed to type correctly the same two samples for GATA C4. By sequence analyses, two different GATA C4 allele structures were found. One control sample (allele 21) and two questioned samples (allele 22, correctly typed by all the laboratories, and allele 25) presented the following repeat structure: (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)n, but different from the one found for allele 26 in one sample included in this exercise, as well as in the second control sample (allele 23), namely (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)2(TGTA)2(TCTA)n. The collaborative exercise results proved that both Y-tetraplexes produce good amplification results, with the advantage of being efficiently typed using different separation and detection methodologies. However, since GATA C4 repeat presents a complex structure, with alleles differing in sequence structure, efficient denaturing conditions should be followed in order to avoid typing errors due to sizing problems. 相似文献
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This paper defines the key values, principal participants and operational requirements that characterise a strategically relevant global issue management process. It is the authors' intention to reveal and provide support for the most important ‘truth’ about this great discipline: ‘Excellence and relevance is determined by the optimal mix of dedicated, focused and highly imaginative people with processes that unleash their remarkable innovative potential. You need both.’ Copyright © 2002 Henry Stewart Publications. 相似文献
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