The determination of benzodiazepines in biological objects on the Milichrome microcolumn liquid chromatograph]

1,4-benzodiazepines and benzophenols, the products of their hydrolytic cleavage, were measured by high-pressure liquid chromatography in expert material (stomach, liver, intestine, kidney samples). Milichrome microcolumn liquid chromatograph, designs 1 and 2, was employed, with a chromatography column packed with Separon C18 (5 microns) sorbent. A mixture of (NH4)2HPO4 (0.05 M) and acetonitryl in volume ratio 65:35 was used as the mobile phase in native compound analysis and that in volume ratio 45:55 for benzophenols and medazepam separation. The external standard method was used for quantitative estimation.     

The use of immunoenzyme analysis for determining ABH antigens in traces of saliva and sperm]

The authors suggest a simple sensitive technique for enzyme immunoassay (EIA) of ABH antigens in saliva and semen. A two-staged dot blot solid-phase EIA on nitrocellulose membranes was employed with anti-ABH monoclonal antibodies obtained in immunization of mice with human red cells. 4-chloro-1-naphthol substrate solution was used to visualize the peroxidase label. The results of analysis of salivary and spermatic samples obtained from donors of various groups evidence that this EIA variant may be useful in forensic medicine.