Detection of a primer-binding site polymorphism for the STR locus D16S539 using the Powerplex 1.1 system and validation of a degenerate primer to correct for the polymorphism

Quality assurance samples submitted from the NCSBI as part of a contract with TBTG to outsource DNA Database samples showed unexpected discrepancies for the locus D16S539 when all other loci yielded identical results. Discrepancies observed included allele drop out and an imbalance in sister alleles with samples returned from TBTG. This led to a comprehensive review of the technical procedures used between the two laboratories to determine the cause of the discrepancies noted for the locus D16S539, since both laboratories were using the PowerPlex 1.1 typing kit from the Promega Corporation. The NCSBI and the TBTG utilize different extraction methods (organic extraction vs. FTA) and amplification conditions (AmpliTaq vs AmpliTaq Gold), respectively, so the exact cause of discrepancy observed was not immediately apparent. Experiments at the NCSBI associated the observed allele drop out and the imbalance of the sister alleles with the use of AmpliTaq Gold and a hot start procedure. Sequencing data revealed that a point mutation resides on the D16S539 primer-binding site that reaches polymorphic levels in African-American populations. This led to the development of a degenerate primer by the Promega Corporation to detect "missing" alleles when AmpliTaq Gold is used. The degenerate primer was then thoroughly tested to show its efficacy in detecting the "true" D16S539 profile when used.     

Primary Attachment to Parents and Peers during Adolescence: Differences by Attachment Style

This study examines the nature of adolescent attachment to parents and peers during adolescence. A projective measure was used to classify 99 11th and 12th grade students into secure, insecure dismissing, and insecure preoccupied attachment groups. Respondents identified their primary attachment figure by nomination and by rating the level of attachment support they received from mothers, fathers, best friends, and boy/girlfriends. On average, parents and peers were equally likely to be identified as primary attachment figures but individual preference was strongly tied to attachment style. Secure adolescents significantly favored mothers over best friends, boy/girlfriends, and fathers. Although secure adolescents with romantic partners rated mothers lower on attachment support, none of the adolescents from this group nominated a boy/girlfriend as their primary attachment figure. In contrast insecure adolescents indicated a strong preference for boy/girlfriends and best friends as their primary target for attachment and nearly a third of dismissing adolescents identified themselves as their primary attachment figure. Findings are discussed in terms of individual differences in attachment during adolescence.     

Halogenated solvent interactions with N,N-dimethyltryptamine: formation of quaternary ammonium salts and their artificially induced rearrangements during analysis

The psychoactive properties of N,N-dimethyltryptamine (DMT) 1a are known to induce altered states of consciousness in humans. This particular attribute attracts great interest from a variety of scientific and also clandestine communities. Our recent research has confirmed that DMT reacts with dichloromethane (DCM), either as a result of work-up or storage to give a quaternary N-chloromethyl ammonium salt 2a. Furthermore, this was observed to undergo rearrangement during analysis using gas chromatography-mass spectrometry (GC-MS) with products including 3-(2-chloroethyl)indole 3 and 2-methyltetrahydro-beta-carboline 4 (2-Me-THBC). This study further investigates this so far unexplored area of solvent interactions by the exposure of DMT to other halogenated solvents including dibromomethane and 1,2-dichloroethane (DCE). The N-bromomethyl- and N-chloroethyl quaternary ammonium derivatives were subsequently characterised by ion trap GC-MS in electron and chemical ionisation tandem MS mode and by NMR spectroscopy. The DCE-derived derivative formed at least six rearrangement products in the total ion chromatogram. Identification of mass spectrometry generated by-products was verified by conventional or microwave-accelerated synthesis. The use of deuterated DCM and deuterated DMT 1b provided insights into the mechanism of the rearrangements. The presence of potentially characteristic marker molecules may allow the identification of solvents used during the manufacture of controlled substances, which is often neglected since these are considered inert.     

Female and male sex offenders: a comparison of recidivism patterns and risk factors

Few studies have empirically validated the assertion that female and male sex offenders are vastly different. Therefore, utilizing a matched sample of 780 female and male sex offenders in New York State, the current study explored differences and similarities of recidivism patterns and risk factors for the two offender groups. Results suggested that male sex offenders were significantly more likely than female sex offenders to be rearrested for both sexual and nonsexual offenses. However, limited differences in terms of risk factors between female and male sex offenders were found.     

Accidental death from a black-powder rifle breech plug

Authentic black-powder muzzle-loader weapons and replicas are used today primarily for hunting game such as deer and hogs. The following is a case presentation of accidental death from cerebral trauma caused by a .45-caliber black-powder-rifle breech plug implanting in the victim's brain.     

The Sources of Terrorist Financing: Theory and Typology

Terrorism costs money. Although the costs of specific operations may be relatively inexpensive, terrorist organizations require much larger budgets in order to function. But how do terrorist groups acquire funding? What explains the variation in the particular sources used by different groups? This article develops a theory of terrorist financing that identifies criteria by which we can evaluate the different sources of terrorist funding, particularly in terms of their advantages and disadvantages to the terrorist group. These criteria are then applied across a typology of four primary types of terrorist financing: state sponsorship, illegal activity, legal activity, and popular support.     

The impact of assisted housing developments on concentrated poverty

Abstract

The common wisdom is that assisted housing developments have both a direct and an indirect impact on concentrated poverty. The indirect effects are based on the notion that the negative stereotypes associated with such developments spill over into the surrounding neighborhoods, causing people who can leave to do so or avoid the neighborhood and leaving behind only the more disadvantaged segments of society. An increase in concentrated poverty in the neighborhood surrounding the development results. Prior studies, relying on aggregated data, are consistent with this thesis. The overwhelming majority of the statistical models in my study, however, found these relationships to be spurious. Once individual and macrolevel characteristics were controlled for, the relationships disappeared.

These findings imply that assisted housing developments will not typically contribute to concentration of poverty in surrounding neighborhoods and suggest that much of the negative reaction to assisted housing developments is unwarranted.     

Ebstein Anomaly and Sudden Childhood Death

A 13‐year‐old girl is reported who died suddenly and unexpectedly in her sleep from previously undiagnosed Ebstein anomaly. At autopsy, there was dilatation of the right atrium with marked dilatation of the right auricle and apical displacement of the tricuspid valve into the right ventricular cavity with atrialization of the upper portion of the right ventricle. There were also prominent dysplastic changes in both the septal and posterior leaflets of the tricuspid valve with thickening of the valve and fusion of leaflets to the wall of the ventricle. Histology of the myocardium showed focal, minor microscopic areas of interstitial fibrosis with marked fibrous dysplasia and thickening of the tricuspid valve. Lethal arrhythmias occur in this condition because of the geographical relationship of the conduction system to the abnormal anatomical structures. As adolescents who died suddenly are often minimally symptomatic, cases will rarely present de novo to forensic autopsy.