Postmortem redistribution of digoxin in rats

Adult male Wistar rats were treated with either 0.1 or 3 mg/kg body weight X day of digoxin for five days, then killed and stored at 4 degrees C for 12 h in an attempt to mimic the normal preautopsy procedures in our hospital. In rats treated with 0.1 mg/kg body weight X day, the antemortem serum digoxin concentrations (SDC) were 1.1 +/- 0.4 ng/mL while the 12-h postmortem concentration was markedly increased (16.3 +/- 5.9 ng/mL) (P less than 0.01). In rats treated with 3 mg/kg body weight X day, SDC was not changed significantly (11.2 +/- 4.8 ng/mL antemortem and 13.3 +/- 6 ng/mL postmortem). Postmortem redistribution of digoxin was assessed by injection of 125I-labelled digoxin with or without pretreatment with the unlabelled drug. The results indicate that after death passive redistribution of digoxin may take place. When the SDC are within the therapeutic or low toxic range, digoxin may reenter the blood. High antemortem serum concentrations of digoxin may prevent such passive redistribution. Therefore, antemortem digoxin intoxication cannot be reliably inferred on the basis of high postmortem levels of the drug. Digoxin intoxication can be ruled out when postmortem SDC remain within the therapeutic range. The above changes cast doubt on some of the forensic and cardiologic literature, which has in the past been based on incorrect assumptions concerning postmortem behavior of digoxin.     

The positioning and magnification of faces and skulls for photographic superimposition

In the identification of persons living or dead, or of skulls, the technique of photographic superimposition of laboratory images on existing original portraits has frequently been used. The applicability of the technique and the confidence in the results have always depended very much on the professional acceptance and experience of forensic specialists. Defined preconditions, improved methods and clear-cut directions on how to posture the head or the skull and how to magnify its photographic laboratory image, not only help to standardize and simplify procedures, but also increase the accuracy and credibility of the resulting diagnoses. A systematic approach with standard photographic equipment is presented. The validity of the results is discussed.     

Non-equilibrium pH gradient electrophoresis (NEPHGE) on ultrathin polyacrylamide gels containing separators: improved erythrocyte phosphoglucomutase (PGM) and esterase D (EsD) diagnosis in red cell lysates and bloodstains

Two rapid and reliable electrophoretic techniques for PGM1 and EsD typing on ultrathin polyacrylamide gels are described. They have been based on non-equilibrium pH gradient electrophoresis and on the addition of chemical spacers (EPPS for PGM1 and HEPES for EsD) to the gel mixture.     

TIME TRENDS AND AGE TRENDS IN ARRESTS AND SELF-REPORTED ILLEGAL BEHAVIOR*

National estimates of time trends and age trends in self-reported illegal behavior in the United States are compared with corresponding figures for arrests. The self-report data are from Monitoring the Future, an annual national survey of high school seniors. The analysis of time trends covers 1975 through 1985 for a sample of over 3,000 respondents per year, studied at a constant age. The analysis of age trends covers ages 17 through 23 for samples of 300 to 1,200 per year. Both self-report and arrest measures reveal substantial declines in illegal behavior throughout this age span, with the exception of arrests for assault. Both methods also indicate that rates of assault increased from 1975 through 1985, a finding that has not previously been reported. Time trends for other offenses were erratic.     

Capgras syndrome and dangerousness

This article discusses Capgras syndrome and its association with harmful and potentially harmful behaviors. Phenomenological and psychodynamic analysis of a series of cases will highlight danger signals that may be present in Capgras patients.     

The determination of glyoxalase I phenotypes in human bloodstains

A method is described for the determination of glyoxalase I phenotypes in liquid blood and dry bloodstains. The frequencies of glyoxalase I phenotypes in various populations of S.E. England are included.     

The design of equipment and thermal routines for annealing glass particles

Equipment for annealing casework sized glass particles is described. Investigations were carried out using a simple muffle furnace, a temperature programmed furnace and a metal sample holder. Because they produced a uniform and reproducible thermal environment, best results were obtained from a combination of the temperature programmed furnace and the metal sample holder. Refractive index changes for window and toughened glass specimens were investigated and a simple, reproducible annealing routine for casework was devised.     

In vivo measurements of facial tissue thicknesses in American caucasoid children

Ultrasonic determinations were made of facial tissue thicknesses in 50 healthy American caucasoid children, ranging in age from 4 to 15. Twenty measurements were taken at sites along the median, right sagittal, and right lateral planes. A static scanner was used in the B-mode. Three measurements significantly increased with age, the mid-philtrum (rs = 0.43, p less than 0.01) in females, the mental sulcus (rs = 0.30, p less than 0.05) in males, and the frontal eminence (rs = 0.32, p less than 0.05) in both sexes. Moreover, 25% of the measurements, while not statistically significant, decreased with increasing age. These data provide a basis for facial reproductions in caucasoid children.