Using FastID to analyze complex SNP mixtures from indoor dust

Forensically relevant single nucleotide polymorphisms (SNPs) can provide valuable supplemental information to short tandem repeats (STRs) for investigative leads, and genotyping can now be streamlined using massively parallel sequencing (MPS). Dust is an attractive evidence source, as it accumulates on undisturbed surfaces, often is overlooked by perpetrators, and contains sufficient human DNA for analysis. To assess whether SNPs genotyped from indoor dust using MPS could be used to detect known household occupants, 13 households were recruited and provided buccal samples from each occupant and dust from five predefined indoor locations. Thermo Fisher Scientific Precision ID Identity and Ancestry Panels were utilized for SNP genotyping, and sequencing was completed using Illumina^® chemistry. FastID, a software developed to permit mixture analysis and identity searching, was used to assess whether known occupants could be detected from associated household dust samples. A modified “subtraction” method was also used in FastID to estimate the percentage of alleles in each dust sample contributed by known and unknown occupants. On average, 72% of autosomal SNPs were recovered from dust samples. When using FastID, (a) 93% of known occupants were detected in at least one indoor dust sample and could not be excluded as contributors to the mixture, and (b) non-contributor alleles were detected in 54% of dust samples (29 ± 11 alleles per dust sample). Overall, this study highlights the potential of analyzing human DNA present in indoor dust to detect known household occupants, which could be valuable for investigative leads.     

Intergenerational Continuity of Risky Parenting: A Person-Oriented Approach to Assessing Parenting Behaviors

Limited research has investigated the long-term effects of childhood emotional abuse on later forms of parenting. This study utilized a person-centered approach to explore the relation between retrospectively-reported maternal childhood emotional abuse and observed parenting behaviors during a conflict discussion. Data were collected from 53 caregiver-child dyads with children ages 8–11. Results of a model-based clustering procedure (Mclust; Fraley and Raftery 2006) identified three parenting styles (negative, at-risk, positive) that were based on five observed parenting behaviors (emotion regulation, anger, hostility, psychological control, and psychological unavailability). Results indicated that higher levels of childhood emotional abuse were reported by women in the at-risk and negative parenting subgroups. Mothers in the negative parenting and at-risk parenting clusters exhibited greater levels of emotional abuse when compared to the positive parenting cluster. Possible implications are discussed, and results underscore the importance of emotionally abusive developmental experiences in the understanding of risk for maladaptive parenting behaviors.     

Early Adverse Childhood Experiences and Social Skills Among Youth in Fragile Families

Journal of Youth and Adolescence - Adverse childhood experiences (ACEs) are associated with negative health and behavioral outcomes across the life course, yet little is known about the association...     

Race and Juvenile Incarceration: A Propensity Score Matching Examination

Disproportionate minority contact is an important issue in contemporary juvenile justice. Few studies have directly examined the link between race and judicial decision to incarceration. Using official data from Pennsylvania (n?=?41,561), this study added to this literature in two ways. This study used propensity score matching to obtain a purer estimate of the influence race has on the decision to petition a case to juvenile court. The results indicated that prosecutors use perceptual shorthand in making this decision that hinges on race. Specifically, blacks were more 1.28 times more likely than whites to have their case petitioned to juvenile court.     

‘Any friend of yours is a friend of mine’: investigating the utilization of an interpreter in an investigative interview

Premised on a body of literature suggesting target-interviewer rapport is a critical component of successful interviews, we explored the effect of two interpreter-related variables – the physical placement of the interpreter in the room, and the nature of the relationship between the interpreter and the target – on target-interviewer rapport. A total of 125 bilingual (Spanish/English) participants viewed a mock crime video and were then interviewed, via an interpreter (or not). Interpreters either built rapport with the participant immediately prior to the interview or did not, and were either seated beside the interviewer or behind the target, commensurate with recommendations from training manuals. When the interpreter and target engaged in a short rapport-building session prior to an investigative interview, the target rated their interaction with the interviewer less negatively compared to when rapport building did not occur. Furthermore, when the interpreter sat behind the target, the target viewed the interaction more negatively than when the interpreter sat beside the interviewer (triangular configuration). These findings suggest ways in which interpreters can be utilized more effectively, especially in terms of seating configuration, rapport development between a target and interpreter, and importantly, the potential for that target-interpreter rapport to transfer to the target-interviewer relationship.     

Murder Followed by Suicide: A Newspaper Surveillance Study Using the New York Times Index

Abstract: Murder–suicide is a relatively uncommon event but as reported by the New York Times, it has occurred and continues to occur yearly. Previous research has indicated that those who commit murder–suicides tend to be men, are in or have been in an intimate relationship with the victim, victims tend to be women, and a firearm is most likely to be used. This study uses a newspaper surveillance methodology to examine such cases. Articles from the New York Times as found in the New York Times Index were coded, analyzed, and examined. The cases, 166 in total, support the findings from prior research. The trend data was examined by cross tabulations and chi‐square analysis. The findings suggest that murder–suicides are rare events and when they occur they usually involve a male perpetrator and an intimate partner victim who is either a wife or girlfriend with the event occurring in a private home. A firearm is the most commonly used method for both murders and suicides, particularly if there was more than one murder victim. The authors conclude by suggesting that future research should focus on using the forthcoming data resource in the CDC’s National Violent Death Reporting System (NVDRS) to examine the occurrence of murder–suicide.     

The Balanced Approach [Revisited]

The authors of the original Balanced Approach, Dennis Maloney, Dennis Romig, and Troy Armstrong, outlined a philosophy of balanced attention to the principles of community protection/public safety, youth accountability, and competency development through individualized assessment and treatment and holding the system accountable to the community and the youth served. A majority of states’ juvenile code purpose clauses reflect this approach, later known as Balanced and Restorative Justice (BARJ). Presented here are excerpts from the 1988 Juvenile & Family Court Journal issue 39 (3) that first presented the Balanced Approach and a brief overview of key features of juvenile justice at the time to lay a foundation for the other articles in this special issue.