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51.
Nelson MS Levedakou EN Matthews JR Early BE Freeman DA Kuhn CA Sprecher CJ Amin AS McElfresh KC Schumm JW 《Journal of forensic sciences》2002,47(2):345-349
Quality assurance samples submitted from the NCSBI as part of a contract with TBTG to outsource DNA Database samples showed unexpected discrepancies for the locus D16S539 when all other loci yielded identical results. Discrepancies observed included allele drop out and an imbalance in sister alleles with samples returned from TBTG. This led to a comprehensive review of the technical procedures used between the two laboratories to determine the cause of the discrepancies noted for the locus D16S539, since both laboratories were using the PowerPlex 1.1 typing kit from the Promega Corporation. The NCSBI and the TBTG utilize different extraction methods (organic extraction vs. FTA) and amplification conditions (AmpliTaq vs AmpliTaq Gold), respectively, so the exact cause of discrepancy observed was not immediately apparent. Experiments at the NCSBI associated the observed allele drop out and the imbalance of the sister alleles with the use of AmpliTaq Gold and a hot start procedure. Sequencing data revealed that a point mutation resides on the D16S539 primer-binding site that reaches polymorphic levels in African-American populations. This led to the development of a degenerate primer by the Promega Corporation to detect "missing" alleles when AmpliTaq Gold is used. The degenerate primer was then thoroughly tested to show its efficacy in detecting the "true" D16S539 profile when used. 相似文献
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Toward a Victimology of State Crime 总被引:5,自引:3,他引:2
State crimes have been studied by criminologists for nearly three decades. While far from stagnant, research and theory in
this area of criminology have not developed at the pace one may have expected a decade ago. In an attempt to rejuvenate the
study of state crime, we first identify and review the various types of victims and victimizers of state crime identified
in the criminological literature. By employing a previously created typology of state crime, we discuss how individuals and
groups of individuals can be identified as state crime victims in both domestic and international contexts. We then highlight
the common themes involved in the victimizations, and offer six inductively generated propositions intended to facilitate
future developments in the victimology of state crime.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Policing Prostitution: Ten Years On 总被引:2,自引:0,他引:2
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