Y-chromosomal STR haplotypes in a population sample from continental Greece, and the islands of Crete and Chios

Eight Y chromosome short tandem repeat (STR) polymorphisms (DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, and DYS393) were analyzed in the sample of 117 unrelated Albanian males living in Kosovo. A general STR allelic frequency pattern in the Albanian population from Kosovo corresponds to other European populations. Fourty six haplotypes were observed in single copy. The most frequent haplotypes were (DYS19-DYS385-DYS389I-DYS389II-DYS390-DYS391-DYS392-DYS393) 14-11/11-13-29-24-11-13-13 (10.26%), 14-14/17-12-28-24-10-11-12 (9.40%), 13-16/18-13-30-24-10-11-13 (9.40%), and 14-17/17-13-31-24-10-11-13 (9.40%).     

Extraction of high quality DNA from biological materials and calcified tissues

Calcified tissues, such as bone and tooth, and some other sample types, such as those containing adhesive, present a challenge to standard extraction protocols. We have developed a lysis reagent, BTA™ lysis buffer, which is designed for use with PrepFiler™ Kit reagents. The BTA™ lysis buffer disrupts calcified tissue matrices and achieves effective extraction of DNA from pulverized bone and tooth samples. In addition, the BTA™ lysis buffer mildly but efficiently extracts DNA from challenging substrates like tape, chewing gum, and cigarette butts and, as with bone and tooth, DNA from these lysates is purified using established PrepFiler™ reagent extraction protocols.We successfully extracted DNA from powdered human bone samples, chewed gum and smoked cigarettes using BTA™ lysis buffer. Extraction yields for bone, gum and cigarette samples tested were consistent and reproducible. This extraction method efficiently removed potential PCR inhibitors from all samples tested, and C_T values for the internal PCR control of Quantifiler^® Human DNA Quantification Kit were consistent and within the normal range. The DNA extracted from these samples also provided conclusive profiles that were free of PCR artifacts when amplified using the AmpF?STR^® Identifiler^® PCR Amplification Kit. The protocol is easily adapted for automation.     

Optimization and validation studies of the Mentype Argus X-8 kit for paternity cases

The use of ChrX-STRs is enormous in forensic case as these have proven to be powerful tools, mainly in deficiency paternity cases when the disputed child is female, and also some special cases involving blood relatives, incest cases, fetal typing in abortion material. The Mentype^® Argus X-8 kit is a commercial multiplex system which contains Amelogenin for gender determination as well as gonosomal STR markers (DXS8378, HPRTB, DXS7423, DXS7132, DXS10134, DXS10074, DXS10101 and DXS10135). Validation studies were being performed on blood obtained from the volunteers in Turkish population. In this study, some parameters were taken under consideration for validation like DNA extraction using different protocols, quantitated by using commercially available Invitrogen Qubit Fluorometer, reaction volume validation of Master Mix and the analysis of female/male, female/female and male/male mixtures were performed. The conditions were optimized and validated using GenAmp 9700 and reducing reaction volume from 25 μl to 12.5 μl and 6.5 μl. After reducing the total volume of the reaction, the results were same and there was no effect on peak height and quality when analyzed on ABI 310 genetic analyzer. 2 paternity cases were also performed which gave the same power of discrimination as has been mentioned in Mentype^® Argus X-8 kit.     

Public policy and sequential lineups

The claim that sequential lineups are superior to simultaneous lineups and that our knowledge of sequential lineups is sufficient to warrant their being required by law is reviewed for the validity of both strong and weak claims of sequential superiority, adherence to principles of research design, and the needs of public policy. We conclude, (1) there is little evidence to support the claim that sequential presentation of photos is responsible for lower levels of false identifications, (2) the evidence is weak that the aggregation of factors commonly labeled as the sequential lineup together produce lower levels of false identifications without additional offsetting effects, (3) much of the literature contains several confounds in research design and additional offsetting effects that question its overall utility, (4) recent research shows that the superiority of sequential lineups is restricted to specific ranges on other study design variables, and (5) the corpus of research on sequential lineups does not satisfy the needs of policy sufficiently to justify its mandated use as the required identification procedure throughout the criminal justice system.     

Methods of Choice in Completed Suicides: Gender Differences and Review of Literature

Abstract:  Suicide is an important public health hazard worldwide. A 4-year retrospective study from January 2000 to December 2003 was conducted to identify the favored methods in realized suicides among males and females in the west coastal region of India. During the study period, a total of 539 cases of suicidal deaths were autopsied. Males were predominantly affected (male: female—1.9:1). The age of the victims ranged from 13 to 90 years in males (mean = 40.1 years, median = 37.0 years) and 15 to 85 years in females (mean = 36.6 years, median = 32.0 years). Most favored method of suicide amongst males and females was hanging (36.9%, n  = 199) followed by poisoning (34.7%, n  = 187). Male dominance was apparent for each method of suicide except for self-immolation. Males were relatively more likely to use hanging and poisoning while females were more likely to prefer drowning and self-immolation as methods of suicide. Relatively younger females (mean   = 33.0 years, median = 32.0 years) preferred hanging as a method of suicide when compared to males (mean = 42.4 years, median = 40.0 years). Among females, significantly younger females resorted to hanging when compared to older females who preferred drowning. On investigating the various theories proposed for choice of suicide methods in males and females in different regions we conclude that preference of method of suicide in men and women is complexly determined. In this region, availability, accessibility, popularity, and socioacceptability seem to be the major determinants in the choice of methods among males and females rather than violence associated and lethality of the method. Females were as likely to use lethal and violent methods as males in this region.     

Unintended consequences: experimental evidence for the criminogenic effect of prison security level placement on post-release recidivism

Most prison systems use quantitative instruments to classify and assign inmates to prison security levels commensurate to their level of risk. Bench and Allen (The Prison Journal 83(4):367-382, 2003) offer evidence that the assignment to higher security prisons produces elevated levels of misconduct independent of the individual’s propensity to commit misconduct. Chen and Shapiro (American Law and Economics Review, 2007) demonstrate that assignment to higher security level among inmates with the same classification scores increases post-release recidivism. Underlying both of these claims is the idea that the prison social environment is criminogenic. In this paper we examine the theoretical premises for this claim and present data from the only experiment that has been conducted that randomly assigns inmates to prison security levels and evaluates both prison misconduct and post-release recidivism. The experiment’s results show that inmates with a level III security classification who were randomly assigned to a security level III prison in the California prison system had a hazard rate of returning to prison that was 31% higher than that of their randomly selected counterparts who were assigned to a level I prison. Thus, the offenders’ classification assignments at admission determined their likelihood of returning to prison. There were no differences in the institutional serious misconduct rates of these same prisoners. These results are contradictory to a specific deterrence prediction and more consistent with peer influence and environmental strain theories. These results also raise important policy implications that challenge the way correctional administrators will have to think about the costs and benefits of separating inmates into homogeneous pools based on classification scores.