Genetic markers in human bone: I. Deoxyribonucleic acid (DNA) analysis.

Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a "per milligram of starting tissue" basis. DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different single-locus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQ alpha phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3' to the human apolipoprotein B (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization.     

The Institutional Basis of Secessionist Politics: Federalism and Secession in the United States

According to the literature on secession, the most importantdeterminant of secessionist sentiment is a high level of grievanceexperienced by the would-be secessionist group. However, therationale behind using secession (as opposed to another strategy)as a grievance-amelioration strategy remains almost completelyunexplained. This article contends that the institutional settingin which political conflict and grievance are experienced playsa crucial role in whether secession is conceived of as a viableoption. This contention is tested by examining the institutionalenvironment of federalism and the role it played in secessionin the United States in 1860 and 1861. The United States wasuniquely vulnerable to secessionist pressures—as are allfederal states, potentially—because of institutional structuresthat gave constituent states a high level of institutional authorityand capacity; this, in turn, contributed to a discourse of states'rights that included the contested right to secede.     

From hegemonic to inclusive masculinities in English professional football: marking a cultural shift

Abstract

In this article, we conduct a generational study of measuring progress towards the inclusion of homosexuality in professional sport. We accomplish this by interviewing an openly gay football player, Anton Hysén, in the year 2017, using the precise same questions about the relationship between his homosexuality and sport that were asked of the first openly gay professional footballer in 1992, Justin Fashanu. We thus measure difference in attitude according to the 25 years that have elapsed between the interviews. Our results emphasize two themes: First, the near-total acceptance of being gay within professional football today, compared to 25 years ago; and second, the social praise that is levied upon today’s openly gay sportsmen, compared to the social stigma placed upon Fashanu.