Detecting psychoactive drugs in the developmental stages of mushrooms

The following questions regarding the detection of psychoactive drugs in mushrooms are addressed: At what stage of the mushroom development can the psychoactive drugs psilocyn and psilocybin be identified, and what effect does light have on the growth of these mushrooms. To answer these questions, Psilocybe cyanescens Wakefield mushrooms were grown from their spores in a controlled setting. At various times of their development, samples were taken and analyzed for psilocyn and psilocybin. Knowing what stage of development the psychoactive drugs can be identified may be useful to law enforcement personnel and forensic chemists. Methanolic extracts of various samples were analyzed by TLC and by GC/MS. It was determined that the mycelium knot stage of the mushroom was the earliest stage at which the psychoactive drugs could be detected. It was observed that light affected the time of development and the appearance of these mushrooms.     

Child physical abuse and self-perceived social isolation among adolescents

This research examines the connection between physical abuse and social isolation. Using data from the National Youth Survey, a measure of self-perceived social isolation was constructed indicating the extent to which respondents feel detached from their friends and from school. Those who had experienced violence were predicted to be more isolated than those who had not. Results strongly supported the hypothesis, controlling for theoretically relevant variables. Explanation is provided in terms of damage to attachment skills, social competence, and self-esteem concomitant to being a victim of abuse. Males were more socially isolated than females, and Hispanics more than Whites. Children with involved parents were less socially isolated; those whose parents experienced normlessness were more isolated. Children who recently experienced a stressful event or were from riskier neighborhoods were more isolated. The number of children in the family was positively related to isolation. Social isolation decreases between seventh and eighth grades.     

An unusual homicide case with subsequent suicide

In the medicolegal literature reports on homicides followed by suicide, especially in elderly people, are rare. In the present case, the victim, a 79-year-old woman, showed a rather unusual injury pattern: 3 gunshots to the head (with only 2 entrance wounds and 3 intracranial projectiles) and 2 stabs from a knife. The tools used for inflicting the stab wounds (a hunting knife and a double-edged dagger) were left in the victim's body after the offense. The 89-year-old husband hanged himself with an aerial cable after killing his wife. The police investigations and the autopsy findings suggested a combined homicide-suicide. The motive for the offense could be a so-called delusion of poverty associated with symptoms of depression.     

The effect of luminol on presumptive tests and DNA analysis using the polymerase chain reaction.

This study was designed to test the following factors involved with processing luminol treated bloodstained evidence: 1) The reactivity of other presumptive chemical color tests, phenolphthalin (PT) and tetramethylbenzidine (TMB), following the application of the light emitting luminol presumptive test. 2) The effect of different cleanings of various bloody substrates on the luminol test. 3) The effect of different cleanings of various bloody substrates on the ability to obtain DNA suitable for PCR testing. 4) The ability to extract DNA from luminol treated bloodstained substrates using three extraction techniques. 5) The effect of spraying washed and unwashed bloodstains on various substrates with luminol on the ability to correctly type the DNA using PCR. Our findings indicated that luminol did not adversely effect the PCR testing and did not interfere with the PT and TMB presumptive tests for blood. It was determined that the substrate and the method of cleaning were the major factors affecting DNA yield and the ability to type the bloodstains using PCR based technologies.     

Performance Standards and Evaluation

The benefits of performance monitoring for public services are identified as greater transparency of objectives and accountability for results. This article considers recent experience in developing standards to evaluate efficiency and effectiveness of public services. In broad terms this is being done through performance information which is a much more encompassing concept than standards or indicators. Such information is an element of an accountability framework that provides greater management autonomy and flexibility and extends to the private sector which is increasingly providing, or involved with the provision of, public services.     

A review of low template STR analysis in casework using the DNA SenCE post-PCR purification technique

LGC has developed a method for analysing low-level DNA samples called DNA SenCE (Sensitive Capillary Electrophoresis) based on post-PCR treatment of standard 28-cycle SGMplus PCR product and demonstrated to be equally effective at enhancing profiles as 34-cycle PCR. The method has been validated and accredited and used in casework since July 2007. Inherent in the method is the initial generation of a standard 28-cycle SGMplus profile so a direct comparison of standard and DNA SenCE results for all casework is possible. Here we review DNA SenCE casework, reporting the magnitude of peak enhancement and stochastic effects seen in the DNA SenCE profiles.     

Victim's scalp on the killer's head. An unusual case of criminal postmortem mutilation

A case of unusual postmortem mutilation of a victim's body is presented. After killing his father, the son decapitated his body and dissected the scalp free, forming a mask of the father's head and neck. The young man wore the scalp-mask over his own head to imitate the father. The motive of the murder was revenge, and the postmortem mutilation was the realization of the perpetrator's fantasies, symbolically representing a penalty for the reprehensible past life of his father.     

Evaluation of haplotype discrimination capacity of 35 Y-chromosomal short tandem repeat loci

The haplotype discrimination capacity of the 9 Y-chromosomal short tandem repeat (Y-STR) loci comprising the so called minimal haplotype together with additional 26 recently described single-copy Y-STRs was evaluated within 391 males from Germany, The Netherlands, and Turkey. The aim of this study was to identify the minimum number of Y-STRs needed in addition to the recommended 9 minimal haplotype loci or the 11 SWGDAM loci for individualizing male lineages. Highest gene diversities were shown for DYS385 loci, DYS449, DYS481, DYS570, DYS447, DYS576, DYS389-II, and DYS390 (D=0.7518-0.8746). The five Y-STRs DYS447, DYS449, DYS481, DYS570, and DYS576 comprised the smallest set of loci in addition to the previously recommended standard Y-STRs leading to the individualization of all males from each single population group. Complete resolution of the pooled population was achieved by the additional genotyping of two further loci, DYS446 or DYS505 and DYF406S1 or DYS522.     

Zu den verfahrensrechtlichen Implikationen der Kompetenzverteilung zwischen der EG und den Mitgliedstaaten im Bereich der Außenbeziehungen

Grundsätzlich finden in Bezug auf die Kompetenzverteilung zwischen EU und Mitgliedsstaaten für die Verhandlung völkerrechtlicher Verträge und den Erlass von „Durchführungsbestimmungen“ im Rahmen völkerrechtliche Vertragsreihe die allgemeinen Grundsätze der Kompetenzverteilung im Bereich der Außenbeziehungen Anwendung. Diese sind aber mit Blick auf die spezifische Problematik von Verhandlung und Durchführung völkerrechtliche Verträge einer Reihe von Präzisierungen zugänglich, die im Folgenden entwickelt werden. Diese Fragestellung ist allgemein im Recht der Außenbeziehungen von Bedeutung; im Umweltbereich ist sie aber wegen der Fülle völkerrechtlicher Verträge der Gemeinschaft und ihrem Charakter als i.d.R. gemischte Verträge besonders relevant. Astrid Epiney ist Professorin für Europarecht, Völkerrecht und Öffentliches Recht an der Universität Fribourg (CH) und geschäftsführende Direktorin des Instituts für Europarecht der Universitäten Bern, Neuenburg und Fribourg. Domènique Gross ist wissenschaftliche Mitarbeiterin des Instituts für Europarecht.* Der Beitrag wurde im Zusammenhang mit einem vom Schweizerischen Nationalfonds zur Förderung der wissenschaftlichen Forschung mitfinanzierten Projekt über die Kompetenzverteilung im Außenbereich zwischen EG und Mitgliedstaaten im Umweltbereich verfasst.