The use of DNA stabilizing solution to enable room temperature storage and transportation of buccal and trace sample swabs

It is proposed that a DNA stabilizing solution (DNA Genotek Inc.) designed to preserve DNA in saliva samples at room temperature can be extrapolated to the storage of swab heads. The aim of this study was to evaluate the effectiveness of the solution for the preservation of reference swabs (buccal) and trace samples (facial swabs). To this end, the solution was used during a twin-site DNA transfer project assessing background levels of carer DNA present in children. Tubes containing 400 μl of solution were used to store and transport swab heads. At the laboratory, samples were extracted using the QIAamp DNA Mini Kit (Qiagen), quantified using the Quantifiler Duo Kit and profiled using the AmpF?STR^® SGM Plus^® PCR Amplification Kit (both Applied Biosystems). Twenty-eight PCR cycles were applied to all samples. Thirty-four cycles or a longer electrophoresis injection time was applied to trace samples where necessary. All Reference swabs produced high quantities of DNA and full DNA profiles after 28 cycles. Profile morphology indicated good quality DNA with no degradation. Of the trace samples, sufficient profiles were achieved to study the transfer of carer DNA making the solution fit for continued use in this project. DNA stabilizing solution enables the storage and transportation of swabs without freezing. This is convenient, reduces transportation costs and enables instant analysis of samples upon arrival at the laboratory. This is a useful alternative for a multi-site research project as well as a reliable storage tool for use in remote areas.     

The characterization of automobile body fillers

Body fillers are sometimes encountered with paint evidence from hit-and-run accidents. Little forensic research has been conducted and published on the subject since 1986. The objective of this study was to determine if chemical and physical differences in body fillers from various manufacturers existed and could be identified. Thirty-three samples of light-weight automobile body fillers and spot putties were obtained. The fillers and putties were compared using light microscopy, infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray spectrometry (SEM-EDX), and pyrolysis gas chromatography (pyGC). Results from fourier transform infrared spectroscopy analysis placed the samples into five groups and differentiated six samples. Light microscopy placed the samples into one of five color groups. PyGC placed the samples into three groups and differentiated one sample. SEM-EDX placed the samples into four groups and differentiated 13 samples. Using these analysis methods, 19 of the 33 samples could be discriminated. The best discriminatory tool was found to be SEM-EDX.     

The State of the State: Comparing Governance in China and the United States

How do two world powers with starkly different governing systems share common accountability problems, and why? Does the cycle of administrative reform produce additional need for reforms that come full circle, eventually exacerbating problems without solution? Yongfei Zhao and B. Guy Peters of the University of Pittsburgh examine governance in the United States and China and point out that conflicts in government functions unique to each country result in strikingly similar accountability issues.     

Comparing public servants' behavior in South Korea and the United States: How emotional labor moderates the relationship between organizational commitment and job performance

The relationship between organizational commitment and job performance matters in public service delivery, and so does the emotive dimension of the citizen—state interaction. However, public management theories and principles must take national culture into account, because comparative investigation reveals limits to generalizability across cultures. This study provides an example. It examines the link between commitment and performance as well as the moderating effect of emotional labor for public service workers in Korea and the United States. Probably due to the differing effect of collectivist versus individualist norms, findings reveal that authentic emotive expression bolsters the relationship between organizational commitment and job performance in Korea, while its opposite, inauthentic emotive expression weakens the link between commitment and performance in the United States. 공공서비스를 전달하는 과정에서 조직몰입과 직무성과의 관계는 중요한 문제이며, 이에 못지않게 시민-국가 상호작용에 있어서 감정적 차원 또한 중요한 문제이다. 공공관리 이론이나 원리를 연구할 때 국가차원의 문화를 고려할 필요가 있는데, 이는 비교연구의 수행이 서로 다른 문화들을 일반화하는 데 따른 한계점을 파악할 수 있게 해 주기 때문이다. 본 연구는 이러한 측면에서 하나의 본보기를 제시한다. 이 연구는 몰입과 성과의 관계를 고찰할 뿐만 아니라 감정노동의 조절효과를 미국과 한국의 공공부문 종사자들을 통하여 살펴본다. 본 연구의 결과는 한국의 공공조직에서 진성 감정표현이 조직몰입과 직무성과를 강화해 줄 수 있으며, 이와는 대조적으로 미국의 공공조직에서는 비진성 감정표현이 몰입과 성과의 관계를 약화시킬 수 있다는 점을 보여준다. 이러한 결과는 집단주의 문화와 개인주의 문화가 서로 다른 영향을 미친 것으로 보인다.     

Semi-peripheral countries and the invention of the ‘Third World’, 1955–65

Revisiting the events leading to the collapse of Third World summitry in 1965, this article proposes that the rise and fall of Third World unity efforts in the years 1955–65 originated from the unsuccessful attempt by industrialised Afro-Asian (aa) countries to turn unindustrialised aa states into their export markets. As a case study, this article explores Egypt’s economic foreign policy towards other aa countries and its activity within the aa and the Non-aligned movements, and compares Egyptian strategy in this field with that of China and Ghana.     

"No Soft Touch": Romani Migration to the U.K. at the Turn of the Twenty-first Century

This paper discusses Romani migration to the U.K. from Central and Eastern Europe (CEE) in the closing years of the twentieth century, with particular reference to the Czech and Slovak Republics. These case studies were chosen to illustrate wider points because they are the best documented, particularly with regard to illuminating sociological research on motivations for migration. Comparisons with similar migration to Canada shed further light on the situation. Refugees from these CEE countries have met a hostile reception in the U.K. It is argued here, however, that popular ignorance alone does not provide a sufficient explanation for this hostility: rather, the condemnation of Romani asylum seekers is seen as an expression of a deep-rooted and long-standing anxiety in the U.K. about immigration and its potential consequences. In spite of their relatively insignificant numbers, Roma have acted as convenient motifs in this ongoing discourse, being assigned a prominent symbolic role at a time of heightened political sensitivity.     

From france to new france

The history of family names is directly linked to the history of populations. In the French-Canadian case, this history has two main features: a small number of founding members of a population, and a rapid increase due to a high reproduction rate, without any significant new influx of immigrants. Other factors intervened in the success of certain names: differential reproduction rates between regions, how early the name was established; and the use of surnames, which in some cases replaced the original name. With respect to names, France and New France were very different. Research in France on the names of a sample of pioneers going to the New World shows little expansion of several names, particularly in the immigrants' region of origin.