Y-chromosomal STR haplotypes in two endogamous tribal populations of Karnataka, India

POPULATION: Approximately 5.0 mL of blood sample was collected from a total of 150 men belonging to two tribal populations of coastal Uttar Kannada district of Karnataka, with their informed written consent. Both the populations are endogamous and they belong to the Dravidian linguistic family. Halakki is a tribal group having a population size of c. 3383. They claim that they originally belong to Gujarat and Rajasthan, and migrated through Andhra Pradesh to Karnataka. Kunabhi is also a tribal population, c. 35,214 in number. They were hunters and gatherers but presently they practice agriculture.     

Enhancing security of fingerprints through contextual biometric watermarking

This paper presents a novel digital watermarking technique using face and demographic text data as multiple watermarks for verifying the chain of custody and protecting the integrity of a fingerprint image. The watermarks are embedded in selected texture regions of a fingerprint image using discrete wavelet transform. Experimental results show that modifications in these locations are visually imperceptible and maintain the minutiae details. The integrity of the fingerprint image is verified through the high matching scores obtained from an automatic fingerprint identification system. There is also a high degree of visual correlation between the embedded images, and the extracted images from the watermarked fingerprint. The degree of similarity is computed using pixel-based metrics and human visual system metrics. The results also show that the proposed watermarked fingerprint and the extracted images are resilient to common attacks such as compression, filtering, and noise.     

Species identification from dried snake venom

Illegal trade in snake parts has increased enormously. In spite of strict protection under wildlife act, a large number of snakes are being killed ruthlessly in India for venom and skin. Here, an interesting case involving confiscation of crystallized dried snake venom and subsequent DNA-based species identification is reported. The analysis using the universal primers for cytochrome b region of the mitochondrial DNA revealed that the venom was extracted from an Indian cobra (Naja naja). On the basis of this report, the forwarding authority booked a case in the court of law against the accused for illegal hunting of an endangered venomous snake and smuggling of snake venom. This approach thus has immense potential for rapid identification of snake species facing endangerment because of illegal trade. This is also the first report of DNA isolation from dried snake venom for species identification.     

Determination of the hand from single digit fingerprint: a study of whorls

In this investigation 400 bilateral rolled fingerprints of Punjabi Jat males have been examined for the determination of the hand from single digit fingerprint from whorl patterns. Direction of the flow of the apex ridges, rotation of centrally circular ridges, angles between the core and the delta, ridge tracing, ridge counting and the position of the perpendiculars drawn between the delta and the core are useful for establishing in most of the cases the hand and finger involved from the fingers having whorl pattern.     

Molecular insight into a wildlife crime: the case of a peafowl slaughter

We report a recent case in which a wildlife warden had suspected that some people had killed and cooked a peacock. Cooked meat, intestine of bird and the wooden block used for chopping were seized from the site of crime and forwarded to our laboratory for DNA testing. Mitochondrial cytochrome b sequence analysis revealed that the cooked meat and remnants of the bird were of a chicken, but the DNA obtained from the wooden block was of an Indian Peafowl (Peacock) testifying that the wooden chopping block was used to chop the meat of an endangered bird, thus bringing to light a wildlife crime.     

Genetic profile of nine autosomal STR loci among Halakki and Kunabhi populations of Karnataka, India

POPULATION: Blood samples were collected from a total of 84 healthy and unrelated Halakki (44) and Kunabhi (40) populations, with their informed written consent. The geographic location of the sampled area is shown in Fig. 1. Both the populations are endogamous, and they belong to Dravidian linguistic family. Halakki is a tribal group having a population size of approximately 3383. They claim that they originally belong to Gujarat and Rajasthan, and migrated through Andhra Pradesh to Karnataka. Kunabhi is also a tribal population, who are approximately 35,214 in number. The male Kunabhi can be identified by their tattoo marks. A necklace is the symbol of married women. They were hunters and gatherers, but at present they practice agriculture.     

Identification of wires and the cutting tool by scanning electron microscope

The cut ends of aluminium and copper wires made by different pliers have been compared under the scanning electron microscope to correlate the two pieces of a wire and to identify the cutting tool. The mechanism of producing the cut ends has been discussed. Matching of extrusion marks and correspondence of breakages at the edges of the cut ends were observed. Correspondence between the fine striations was observed on the opposite faces. These features are put forward for assigning a cut wire to a source.The ends produced by repeatedly cutting a wire by the same portion of the edge of a pair of pliers have been compared. It was found that the striations and fracture zones were reproduced on the respective surfaces of the consecutive cut ends. To identify the cutting tool in wire theft cases, it is suggested that the trial cut ends can be made by cutting the wire with successive portions of the edge of the suspected tool and compare the surface features with that on the questioned ones.