Evaluating Bioinformatic Pipeline Performance for Forensic Microbiome Analysis*,†,‡

Microbial communities have potential evidential utility for forensic applications. However, bioinformatic analysis of high-throughput sequencing data varies widely among laboratories. These differences can potentially affect microbial community composition and downstream analyses. To illustrate the importance of standardizing methodology, we compared analyses of postmortem microbiome samples using several bioinformatic pipelines, varying minimum library size or minimum number of sequences per sample, and sample size. Using the same input sequence data, we found that three open-source bioinformatic pipelines, MG-RAST, mothur, and QIIME2, had significant differences in relative abundance, alpha-diversity, and beta-diversity, despite the same input data. Increasing minimum library size and sample size increased the number of low-abundant and infrequent taxa detected. Our results show that bioinformatic pipeline and parameter choice affect results in important ways. Given the growing potential application of forensic microbiology to the criminal justice system, continued research on standardizing computational methodology will be important for downstream applications.     

Organizational and Human Factors Affecting Forensic Decision-Making: Workplace Stress and Feedback

Although forensic examiners operate in a stressful environment, there is a lack of understanding about workplace stress and feedback. These organizational and human factors can potentially impact forensic science judgments. In this study, 150 practicing forensic examiners from one laboratory were surveyed about their experiences of workplace stress, and the explicit and implicit feedback they receive. Forensic examiners reported that their high stress levels originated more from workplace-related factors (management and/or supervision, backlogs, and the pressure to do many cases) than from personal related factors (family, medical, and/or financial). The findings showed that a few (8%) of the forensic examiners sometimes felt strong implicit feedback about what conclusions were expected from them and that some (14%) also strongly felt that they were more appreciated when they helped to solve a case (e.g., by reaching a “match” as opposed to an “inconclusive” conclusion). Differences were found when comparing workplace stress and feedback levels across three core forensic science fields (forensic biology, chemistry, and latent prints) and across career stages (early, mid, and late). Gaining insights into the stress factors within a workplace and explicit and implicit feedback has implications for developing policies to improve the well-being, motivation, and performance of forensic examiners.     

Horses for courses: China's accommodative approach to food standard‐setting in response to the internationalization of regulation

This paper examines the evolution of China's food standard‐setting procedures from both domestic and international perspectives, particularly in the context of the internationalization of regulation. After the reform and opening‐up in 1978, state actors and leading enterprises monopolized the process of national food standard setting. With further participation in the global economy in the 21st century, China has become familiar with the international standard‐setting procedures and has modeled its domestic policymaking on these practices. This has resulted in a more transparent, inclusive, scientific, pluralized, and consensus‐based form of decisionmaking. By contrast, the standards of the strategic industries have been harmonized to the standards of international counterparts through a top‐down and authoritarian approach. This paper argues that China uses an accommodative approach, trichotomizing suitable standards and decisionmaking procedures in terms of inclusiveness and transparency, which suits the developmental needs of the domestic market, food export markets, and strategic industries.     

Augusta Webster’s Mother and Daughter and the only child debate

The enduring debate about family size has roots in Victorian England, most notably in literary works addressing the issue of balancing motherhood and a writing career. One Victorian writer and activist, Augusta Webster (1837–1894), directly addressed the issue of family size in her uncompleted sonnet sequence Mother and Daughter (1895), which she began when her only child was a newborn. In this posthumous series of 14-line poems, Webster defends her decision to have one child and, in doing so, challenges popular assumptions that only women with multiple children could be considered ‘complete’, socially-acceptable mothers. Despite her efforts, however, and despite the rising popularity of one-child families, the results of numerous scientific studies and the lingering critiques of mommy blogs make it clear that challenges to mothers of ‘onlies’ remain.     

Configurations of Identity Among Sexual Minority Youth: Context,Desire, and Narrative

Youth with same-sex desire undergo a process of narrative engagement as they construct configurations of identity that provide meaning and coherence with available sexual taxonomies. This article presents a theoretical analysis and four case studies centering on the relationship among context, desire, and identity for youth with same-sex desire. Through an interpretive, holistic analysis of the personal narratives of youth, we examine the integration of same-sex desire, behavior, and identity in the general life story and the selective appropriation of elements of “master narratives” of sexual identity development. Narratives were characterized by challenges to integrate desire, behavior, and identity into a configuration that conformed to the received sexual taxonomy. Implications for theory and further research on sexual identity development are discussed.     

Comparing Sexual-Minority and Heterosexual Young Women’s Friends and Parents as Sources of Support for Sexual Issues

The present study provides a comparative analysis of sexual-minority and heterosexual emerging adult women’s experiences seeking support for sexual issues from parents and friends. Participants included 229 college women (88 sexual-minority women; 141 heterosexual women), ranging from 18 to 25 years of age, who provided written responses to an inquiry about a time they went to friends and parents for support for a issue related to their sexuality. Responses indicated that the majority of participants had sought support from either a parent or a friend and that mothers and female friends were more likely involved than fathers or male friends, respectively. Sexual issues that participants reported discussing with parents and friends were inductively grouped into five categories: dating and romantic relationships, sexual behavior, sexual health, identity negotiation, and discrimination and violence. Issues that were discussed differed based on sexual orientation identity and the source of support (parent or friend); they did not differ by age. Participants generally perceived parents and friends’ responses as helpful, though sexual-minority participants perceived both parents and friends’ responses as less helpful than did heterosexual participants. Overall, results suggest both similarities and differences between sexual-minority and heterosexual young women’s experiences seeking support for sexual issues from parents and friends.