Acute intoxication caused by overdose of flunitrazepam and triazolam: high concentration of metabolites detected at autopsy examination

ABSTRACT: A 52-year-old woman was found dead on the floor of the living room on the first floor of a house, which belonged to the man with whom she shared the house. On visiting the site, her clothes were found to be undisturbed. Packages of flunitrazepam (Silece, 2 mg/tablet) and triazolam (Halcion, 0.25 mg/tablet) were found strewn around the victim. Toxicological analysis was performed, and the concentrations of flunitrazepam, triazolam, and their metabolites in the victim's blood and urine were measured by high-performance liquid chromatography coupled with photodiode array and mass spectrometry. A high blood concentration of 7-aminoflunitrazepam was detected (1,270 ng/g), and further metabolites such as 7-acetamidoflunitrazepam, 7-acetamidodesmethylflunitrazepam, and 7-aminodesmethylflunitrazepam were detected in the blood and urine samples. In addition, 4-hydroxytriazolam and α-hydroxytriazolam were detected in her urine at a concentration of 950 and 12,100 ng/mL, respectively.On the basis of the autopsy findings and toxicology results of high concentrations of both flunitrazepam and triazolam derivatives, the cause of death was determined to be acute intoxication from flunitrazepam and triazolam.     

Usefulness of a toothbrush as a source of evidential DNA for typing

We investigated the usefulness of a toothbrush as a source of DNA for an unidentified cadaver. Ten toothbrushes were obtained from ten individuals along with their peripheral blood. We recovered from 10 to 430 ng of DNA from all but one of the toothbrushes. All ten toothbrushes, including the one containing no detectable DNA by fluorometry, were typed correctly at all of the loci tested, including nine STRs. Three toothbrushes obtained in two actual deaths also identified two victims and one suspect. Therefore, toothbrushes seem to be useful as a source of evidential DNA for personal identification.     

Statistical comparison of dog and cat guard hairs using numerical morphology

Numerical features obtained from the guard hairs of dogs and cats (total 300 hairs per dog or cat) were statistically compared, in an attempt to discriminate between them. Using hairs from each of five mongrel dogs and cats, eight measurements (length (Len), maximum width (MaxWid), cross sectional maximum diameter, cross sectional minimum diameter, cuticular thickness of the cross section and three scale counts per 100 microm length (observed at three positions: distal third (disSC), middle (midSC) and the proximal third (proSC) portions) and five indexes (hair width index (HWI), medulla index (MI), hair index, cuticle index and the difference in scale counts between the distal and proximal parts (defSC)) were examined. The range for each numerical feature overlapped each other extensively, and none of the features permitted a discrimination between dog and cat hairs, based on the values obtained. However, 12 numerical features, except for the midSC, showed a statistically significant difference between dog and cat hairs, as evidenced by a t-test. For the purpose of comprehensively comparing numerical features and statistically discriminating between dog and cat hairs, a discriminant analysis between the two were carried out using a multiple regression analysis. Four types of discriminant functions produced by combining over five numerical features were examined. Dog and cat hairs could clearly be discriminated using any of the discriminant functions. Species discrimination using the discriminant function permitted the species of a dog or cat to be determined, based on the overall morphologies of various numerical features. When experimentally collected test samples were investigated using the discriminant function using Combination-2, consisting of eight numerical features (Len, MaxWid, MI, HWI, disSC, midSC, proSC and defSC), all 10 cat hairs were correctly determined to be cat hair and 22 of 23 dog hairs were correctly identified. This discriminant function produced good results for species discrimination between dog and cat hairs.     

Preliminary study of hair form of Japanese head hairs using image analysis

The use of average curvature measurements for the forensic comparison of curly hairs has been reported, but a method, in which various types of hair form are quantitatively examined and objectively interpreted for hair comparison, has not been reported to date. In the present study, numerical data on hair form from Japanese subjects were obtained by image analysis and a morphological comparison of these head hairs was investigated. Head hairs obtained from eight Japanese males were measured for length (L), distance (D) and area (A) using a Kontron Imaging System KS400. From the three measurements mentioned above, three indexes, L/D, A/D and 2(A/L), were examined. The inter-individual variations for each value were investigated by a t-test and the availability of six values for the forensic comparison of hair form was evaluated by a stepwise linear discrimination analysis. Six values obtained from hair form by an image analysis showed large intra-individual variations. However, these six values were found to be useful for discriminating between two individuals, since the six values showed larger inter-individual variations than intra-individual variations. Discrimination on each comparison using a stepwise linear discrimination analysis was performed for some of the values and the results indicated conspicuous inter-individual variations between the two individuals. On 11 of 28 comparisons, 30 hairs from one individual could be completely distinguished from hairs of another individual, when a two-way comparison was employed. These results confirm that hair form could be quite useful in the forensic comparison of hair morphology, and suggest that numerical data obtained from hair form by image analysis are very important values for constructing a screening procedure for evidential hairs. The use of an objective measure of hair form will be especially useful for Japanese head hairs since they are generally thought to show very limited variation in morphological features.     

Perils of pork‐barrel politics: The birth of the Murayama government

In August 1993, Morihiro Hosokawa's coalition government brought an end to the “1955 Regime” of Liberal Democratic Party‐dominated politics in Japan. Since then, Hosokawa himself has been brought down, as has his successor Tsutomu Hata. After years of stability, Japanese politics are now marked by confusion and indecision that show no signs of abating. In this article, IIPS Research Director Seizaburo Sato, who is also professor of political science at Keio University in Tokyo, points out that whatever happens to the not‐so‐strange bedfellows of the present governing coalition, Japan as a nation is at a historical crossroads, and the decisions it makes in the next few years will have far‐reaching implications.     

Japan's democracy in crisis: The new tide in Japanese politics

Since August 1993, when Morihiro Hosakawa inaugurated his “anti‐LDP” coalition, unstable coalitions have held power in Japan. Popular displeasure with the current state of politics has been evidenced by voter volatility and low electoral turnouts. In two notable cases, “flash candidates “ have assumed office, and, in some local elections, candidates have run unopposed. What does this listless version of dissent bode for Japanese politics? IIPS Research Director Seizaburo Sato is Professor at the Saitama University Graduate School of Policy Science. In this article, he maps out the issues that have left post‐Cold War Japanese politics in disarray and examines various potential scenarios, from a “super party “ larger than the LDP at its zenith, to the birth of a two‐party system.     

Is development studies becoming too brainy? A comparison of World Development Reports

This article argues that two types of “brainisation” hinder development studies researchers in their phronetic understanding of poor people’s realities. It first provides a literature review on two types of knowledge and their differences, as well as two types of brainisation and how they prevent development studies scholars gaining a holistic understanding of the marginalised. Subsequently, a comparative analysis is conducted on two World Development Reports. Finally, alternative scenarios are outlined for the “debrainisation” of development studies and researchers’ mind-sets by learning from the fundamental features of human life.     

Anti-Russian and Anti-Soviet Subversion: The Caucasian–Japanese Nexus, 1904–1945

This article examines the little known history of political collaboration between Caucasian national groups and Japan directed against the Soviet Union in the 1930s. The collaboration, begun at the time of the 1904–1905 Russo-Japanese War, resumed in the 1920s and continued through World War II. The Caucasian groups (Haidar Bammat's ‘Caucasus Group’ in particular) and Japan worked together to pursue their common goal of dismembering the Soviet Union. Their anti-Soviet subversion was real yet achieved few results in the face of extraordinary Soviet security. Nevertheless, Stalin took no chances and terrorised anyone suspected of any possible link to the subversive activity.     

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human red blood cells

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human erythrocytes is described. Specific antibodies were first absorbed with the corresponding cells, then eluted by heating at 53 degrees-55 degrees C for 15 min. The method is simple and highly efficient with a fair recovery of 15.6%-34.4%.     

Molecular analysis of the human esterase D gene ESD(*)Q0(yonago) responsible for incompatibility in a Japanese paternity case

In a Japanese paternity test, an alleged father was excluded only by reverse homozygosity of esterase D (ESD) phenotypes (mother, ESD 1; child, ESD 1; alleged father, ESD 2) out of 43 classical and DNA markers investigated. To solve the aberrant inheritance of the ESD phenotypes observed between them, fragments for all eight coding exons amplified by polymerase chain reaction (PCR) were subjected to DNA analysis. The child and alleged father shared a null allele, originating from ESD(*)1. It was characterized by having TGA for the stop codon instead of TCA for serine at codon 63. Thus, the sharing of a rare null gene, ESD(*)Q0(yonago), increased the probability of paternity.