《唐人街》:海外华人世界的成功展现

进入新千年,凤凰卫视制作播出了大型电视纪录片<唐人街>,首次以纪录片形式透视了海外华人的生存境况,在艺术与市场上都取得了巨大成功.本文对<唐人街>独具特色的制作理念、信息内容、表现方式、传播途径作了全面分析.     

Study on the application of parent-of-origin specific DNA methylation markers to forensic genetics

In paternity test, especially in motherless cases, the allele inherited from father (obligatory gene, OG) often cannot be determined. The paternity exclusion probability (PE) of a genetic marker is reduced considerably. Therefore, it is necessary to develop a new technique, by which the parental origin of alleles can be determined without genealogical analysis. In this paper, we explored the possibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles, choosing the imprinted single nucleotide polymorphism (SNP) locus rs220028 (A/G) as a model system. We typed the SNP by mutagenically separated PCR (MS-PCR). The frequencies of alleles were A = 0.5085, G = 0.4915; the unbiased heterozygosity was 0.5020. In order to discriminate between the maternal allele and paternal allele, post-digestion MS-PCR, a novel PCR based methylation analysis and SNP typing technique was developed and performed on 18 heterozygous children, and the methylated maternal allele was detected specifically. As a pilot study on the use of epigenetic markers in forensic genetics, our results demonstrated the feasibility of using parent-of-origin specific DNA methylation markers to determine the parental origin of alleles.     

加强和改进党政干部教育培训的若干思考

党政干部教育培训是我国教育事业的重要组成部分。宁夏党政干部教育培训工作在培训主体、培训客体和管理环节方面都不同程度地存在着问题,需要树立和实践"大教育、大培训"的新理念,建立健全党政干部教育法规、制度和机制,通过整合教育资源、建设教育培训的主渠道等途径来加以解决。     

新疆毒情分析及对策研究

新疆的毒品问题始于20世纪80年代中后期,经过十几年的发展、蔓延,吸毒人员大量增加,毒品案件成倍上升,“金新月”毒品对我渗透日趋严重,“三股势力”以毒养恐愈发明显,外流贩毒活动愈演愈烈,毒情更加严峻。今后一个时期,新疆受世界毒潮泛滥和国内短时期内毒害难以根除的双重影响,毒情有进一步加剧的可能。为此,要把禁毒工作提升到与打击“三股势力”同等重要的位置抓紧抓好,建立“三大机制”,完善“三库”网络,实现堵源截流,减少毒品危害。     

中西警察学研究的区别与趋同

中国人在引进西方警察科学时不仅改进了我国的警务实践,更深化了警察学的理论研究,创造了警察学。随着我国警察学研究的进一步深化和发展,越来越多的警察学研究者提出创建独立的警察学大学科。但是,中国警察学毕竟不同于西方的警察学。因此,对中西方警察学的研究予以比较,借鉴西方警察学研究的优点,回避其缺陷,探索出我国警察学建设的发展方向就显得极其必要。     

中国东部蒙古族人群15个STR基因座多态性研究

目的调查15个STR基因座在中国东部蒙古族人群中的基因频率分布。方法应用四色荧光标记引物复合扩增技术,对105名东部蒙古族无关个的血样15个STR基因座进行多态性研究。结果在东部蒙古族人群中15个STR基因座偶合率在0.0084～0.2169之间,个体识别概率(DP)在0.7831～0.9916之间,杂合度在0.5619～0.9231之间,三联非父排除率(PE)在0.4490～0.8444之间,多态性信息总量(PIC)在0.5438～0.9178之间,15个STR基因座总TDP值为0.9999999999998,所有基因座经χ2检验符合Hard-Weinberg平衡。结论上述15个STR基因座在东部蒙古族人群中等位基因分布较好,个体识别率高,适合法医个体识别和亲子鉴定。     

两地区医疗纠纷法医学尸检鉴定质量的比较研究

目的研究影响涉及医疗纠纷法医学尸检鉴定质量差异的原因。方法两个具有代表性的法医学尸检鉴定机构所涉及医疗纠纷的相关案例,进行字2检验、t检验等统计学研究。结果通过字2检验发现,在设定的10个指标中,有7个指标存在显著性差异,提示两鉴定单位在“涉及医疗纠纷的法医学尸检鉴定”中检验手段和标准、规范性等均存在极大的差异。结论通过研究,提示法医学鉴定人素质、尸检的标准化和规范化、尸检检材的提取标准化、死因或疾病的诊断标准、会诊制度及法医学鉴定书的书写规范化等是法医学尸检鉴定质量控制和管理的重要环节。     

Forensic identification of dyes extracted from textile fibers by liquid chromatography mass spectrometry (LC-MS)

LC-MS is used for the identification of dyes extracted from textile fibers and the utility of the method for forensic trace analysis is demonstrated. The technique is shown to provide a high degree of chemical structural information, making dye identification highly specific in comparison to optical and/or chromatographic methods of dye analysis. A UV-visible absorbance detector, placed in series before the MS detector, facilitates monitoring the elution of dyes in the presence of other non-dye components extracted from colored textile fibers. In this way, dye identification becomes practical, even when a dye standard is not available for comparison. A set of 22 reference dyestuffs and 10 dyes extracted from textile fibers were analyzed to demonstrate the utility of the method. Six of the extracted dyes corresponded to dyes also contained in the set of 22 reference dyestuffs. Reference dyestuffs were not available for four of the extracted dyes. Triethylamine (TEA) was shown to increase the electrospray ionization-mass spectrometry (ESI-MS) response of dyes containing multiple sulfonated groups.