鹅细小病毒和鸭瘟病毒复合PCR检测方法的建立

根据GenBank中的鹅细小病毒(GPV)和鸭瘟病毒(DPV)基因序列,分别设计合成了针对GPVVP3和DPV UL6基因片段的2对引物,以GPV-GZ1株鹅胚尿囊液和DPV-SD株鸭胚尿囊液的核酸提取物混合液作为模板,经优化反应条件,成功建立了检测GPV和DPV 2种病毒的复合PCR方法.特异性试验结果显示,该方法对GPV-GZ2株与DPV-SC株病毒核酸的扩增均获得550 bp和376 bp的2条特异性目的片段,而对鹅副黏病毒、鸭肝炎病毒、鸭源沙门菌、鸭源巴氏杆菌和鸭疫里默氏杆菌的核酸扩增结果均为阴性;敏感性试验结果显示,该方法对GPV核酸的最小检出量为1.66 Pg,对DPV核酸为0.166 Pg;对人工感染雏鹅的肝组织进行PCR扩增,结果可检测到相应的特异性病毒核酸片段.表明,建立的复合PCR方法具有特异性强、敏感性高、快速简便等特点,可用于GPV或/和DPV临床感染病例的联合检测与鉴别诊断.     

Trajectories of Discrimination across the College Years: Associations with Academic,Psychological, and Physical Adjustment Outcomes

Journal of Youth and Adolescence - Despite growing evidence that racial-ethnic discrimination has a critical impact on college students of color, there is a shortage of longitudinal studies...     

A Closer Look at Peer Discrimination,Ethnic Identity,and Psychological Well-being Among Urban Chinese American Sixth Graders

Recent research suggests that although ethnic discrimination may have negative consequences for psychological well-being among youth of Chinese descent as it does for other ethnic groups, ethnic identity beliefs may buffer against such effects. Data for this study were drawn from the Early Adolescent Cohort Study, an investigation of contextual influences on the social, emotional, and academic adjustment of youth in ethnically diverse New York City middle schools. The present study sample consists of Chinese American (n = 84) and African American (n = 119) sixth graders. Results suggest that Chinese American youths’ own positive affect toward their ethnic group (private regard) was positively associated with higher self-esteem. In addition, the more favorably Chinese American youth perceived that others view their group (public regard), the fewer depressive symptoms they reported. In addition, among Chinese American youth, more favorable public regard attenuated the negative relationship between peer ethnic discrimination and depressive symptoms. The implications of these findings are discussed in light of the commonalities among ethnic and racial minority groups’ experiences of discrimination as well as the unique challenges that Chinese American youth face.

Trajectories of Depressive Symptoms and Suicidality Among Heterosexual and Sexual Minority Youth

Sexual minority youth report higher rates of depression and suicidality than do heterosexual youth. Little is known, however, about whether these disparities continue as youth transition into young adulthood. The primary goals of this study were to describe and compare trajectories of adolescent depressive symptoms and suicidality among sexual minority and heterosexual youth, examine differences in depressive symptoms and suicidality trajectories across sexual orientation subgroups, and determine whether there are gender differences in these longitudinal disparities. Four waves of data from the National Longitudinal Study of Adolescent Health were analyzed using latent curve modeling (N = 12,379; 53 % female). Results showed that the rates of depressive symptoms and suicidality in early adolescence were higher among sexual minority youth than among heterosexual youth, and that these disparities persisted over time as participants transitioned into young adulthood. Consistent with previous cross-sectional studies, the observed longitudinal disparities were largest for females and for bisexually-identified youth. Sexual minority youth may benefit from childhood and early adolescent prevention and intervention programs.     

A dog in the fight

Holly Hughes’ “A Dog in the Fight” is a queer and feminist literary performance that models a feminist politics of performance that stands as alternative to performances of power that come from the officially anointed space of politics. In this piece, Hughes meditates on the queer relationship between US Presidents and their faithful companions, their dogs. Refusing to jump through the “ceiling” to get into the White House, Hughes offers critique of power from her own couch, recounting her hopes and disappointments in the young Obama administration, while forcing a hated former leader (George W. Bush) from the White House into her performance space as she offers up both a critique of his politics and admits to an uncomfortable affiliation with his love of dogs. What emerges from this performance is a critical call to continue an engagement with struggles for social justice from the fecund and powerful places that exist outside of the authorized institutions of politics, no matter what women and men take up positions within these institutions.     

The virtues of specialization: British and American diplomatic reporting on Russia, 1921-39

While important changes were made to the organization of the British diplomatic establishment in the years after 1918, most senior officials remained committed to the ethos of the 'generalist'. In the United States, by contrast, significant efforts were made to inculcate the virtues of professionalism and specialization among members of the Foreign Service. This paper examines the way in which members of the American and British diplomatic establishments monitored developments in the USSR during the interwar years. It concludes that US diplomats were no better than their British counterparts at interpreting developments in Soviet Russia, despite the fact that they were generally better trained to carry out their duties.     

Heterosexism in High School and Victimization Among Lesbian,Gay, Bisexual,and Questioning Students

This study examined relationships between perceived heterosexism in high school policies and programs, social environments, and victimization rates among lesbian, gay, bisexual and questioning (LGBQ) students. Secondary analyses of Internet survey data from a large cohort of LGBQ students (N = 2037; 76% male, 82% White; mean age = 16.07; 56% gay or lesbian; 28% bisexual; 16% questioning) yielded moderate correlations between perceptions of non-discrimination and harassment policies, inclusive programs, and the prevalence and tolerance of anti-LGBQ harassment. The perceived availability of inclusive programs was more closely associated with perceptions of the prevalence and tolerance of harassment in schools than were perceived policies. Victimization was related to perceived policies, programs, and harassment. Perceived harassment partially mediated effects of programs on victimization, but perceived programs also predicted victimization even after controlling for perceived harassment. Moderating effects of demographic characteristics (e.g., gender, race, sexual orientation, and outness) were explored.