New variant for detecting ABO antigens in human bones

AB0 antigens can be extracted from human bone tissue into aqueous solution during boiling and then identified by absorption elution test.     

Comparative analysis fo heroin

A protocol for comparative analysis of heroin making use of chromatographic methods and infra-red spectroscopy is presented.     

Isolation of nitrobenzene from biological material

Toluene is recommended as an agent for extraction of nitrobenzene from biological material. Optimal conditions for nitrobenzene isolation from cadaveric human liver are defined and the results of isolation are quantitatively evaluated.     

Expert significance of evaluating volatile non-metal combustion products in analysis of blood from subjects dead in fire

Qualitative and quantitative composition and time course of formation of toxic substances during burning of polymers have been studied for two polymers, P-53-P and STBO. The most toxic gaseous products of nitrogen combustion are listed: hydrogen cyanide, nitrites, acid nitriles, aldehydes, aromatic carbohydrates, alkanes, and carbon oxide. Two expert conclusions are presented, including analysis of blood and viscera from subjects who died during fire. The following toxic substances were detected: carbon oxide, hydrogen cyanide, nitrites, acrylonitrile, acetonitrile, benzene, and methane in one case and the same products except carbon oxide in the other. The author considers that the list of tested toxic substances should be extended, particularly in cases with negative results of blood testing for carbon oxide and carboxyhemoglobin.     

Forensic medical evaluation of flaws in medical care rendered at public health institutions in the Russian Federation

The authors analyze the flaws in medical care rendered at various public health institutions by the results of 2436 expert committee conclusions, verified at bureaus of forensic medical expert evaluations. The essence and causes of these defects at the hospital and prehospital stages of medical care, rendered by physicians of different specializations and nurses, are analyzed.     

Participation of forensic medics in anthropological examinations of the Orthodox church burials

Examinations of the rudiments of the Russian Orthodox Saints are reviewed. The burial of holy people in Russia is described. The results of examinations of rudiments of the Saints, Reverend Iosaph of Belgorod, Cornilai Padansky, and Arsenii Konevsky, are presented. A detailed algorithm of expert steps is suggested.     

Use of Adobe Photoshop software in medical criminology

Describes the method of comparative analysis of various objects in practical medical criminology and making of high-quality photographs with the use of Adobe Photoshop software. Options of the software needed for expert evaluations are enumerated.     

Morphine-3-D glucuronide stability in postmortem specimens exposed to bacterial enzymatic hydrolysis

Medical examiners frequently rely on the finding of free morphine present in postmortem specimens to assist in certifying deaths associated with narcotics. In vitro hydrolysis of morphine-3-D glucuronide (M3DG) to free morphine was studied using variable specimen pH, initial degree of specimen putrefaction, storage temperature and time, and the effectiveness of sodium fluoride (NaF) preservation. Reagent M3DG was added to opiate-free fresh blood and urine and to autopsy-derived blood specimens. Reagent bovine glucuronidase was also added to certain specimens. Freshly collected and refrigerated NaF-preserved blood produced minimal free morphine, whereas four of five autopsy blood specimens produced free morphine from M3DG. Increased storage time, temperature, and initial degree of putrefaction resulted in greater free morphine generation despite the absence of viable bacteria. Hydrolysis occurring during specimen storage can generate free morphine from M3DG and may result in erroneous conclusions in certifying narcotic deaths.