表达破伤风毒素C片段的重组鼠伤寒沙门菌的构建与鉴定

为构建表达破伤风毒素C片段基因的减毒鼠伤寒沙门菌,将编码破伤风毒素C片段的基因插入到asd 的组成型表达载体pYA3333,转化asd基因突变的E.coliX6212,获得重组质粒pYA3333-TetC,再将重组质粒通过电穿孔法转入宿主菌,构建成表达破伤风毒素C片段基因的平衡致死的鼠伤寒沙门菌X4550(pYA3333-TetC)。免疫印迹试验证实,该重组菌表达的蛋白与预期的目的蛋白一致。重组菌X4550(pYA3333-TetC)的稳定性试验表明,在体外培养100代后,随机挑选的重组菌落全部都能生长,重组菌X4550(pYA3333-TetC)的生长曲线测定表明,X4550(pYA3333-TetC)和X4550(pYA3333)的生长状态基本一致。动物试验证明,该重组菌是安全的,破伤风毒素攻击后能提供良好的保护作用。     

反垄断执法和解中的利益平衡

允许执法者与企业就垄断行为达成和解,是现代反垄断执法的重要特征之一。执法和解反映了企业自治在现代反垄断法上地位的提升,虽能在一定程度上缓和传统执法方式的不足,大大节约执法资源从而促进效率,却易造成公共利益的损失,也可能对相关主体的利益照顾不足;因此,在以和解程序处置垄断案件的同时,必须防止对公共利益的“私相授受”,并对相关主体的利益维护提供制度保障,以实现主体间的利益平衡。     

Conserving Lusheng Cadences

PAIKA is a rural community of the Miao ethnic minority in hilly Guizhou Province.In the local language this name refers to a mountain village that produces the Lusheng,a reed instrument.This village of about 100 households has been known around the province for its Lusheng production for the past five generations or so.Mo Yanxue,62,is from a Lusheng producing family in Paika.He started to     

Treating Their Garbage Right

BEFORE the 1990s most Chinese cities relied on the landfill method to treat their municipal solid waste. But with the expansion of cities and mounting solid waste, the use of land to bury garbage was swiftly becoming too extravagant a solution. In 1985 Shenzhen imported technologies and equipment from Japan to build the country＇s first garbage treatment plant deploying incineration technolozy;     

浅谈城市环境卫生事业的低碳发展

随着经济社会发展和公民环保意识的不断增强,低碳经济越来越引起人们的重视.低碳经济的基本内涵是应用新技术和创新机制,在全社会形成以高能效、低能耗、低污染、低排放为基础的低碳经济发展模式和生活方式,实现经济社会的可持续发展.低碳经济涉及到各个行业、方方面面,作为环卫工作者,应从环境卫生方面积极探索和尝试低碳发展方向.     

Preservation of the Waning Temple Opus

正Hu Qingxue,40,a farm lad from Gu’an County in Hebei Province began in his teens to learn traditional musical notation.At 17 he enrolled in the classes of Zhang Benxing,a master of Jing Music at Beijing’s Zhihua Temple,which dates back to the early Ming Dynasty(1368-1644).Hu is now a government-recognized successor to this national intangible cultural heritage.     

Made in Where？A Wine Provenance Verification System to Root out Counterfeits

CHINA’s first provenance verification system for imported wines was formally unveiled in Shenzhen in November 2012. Its application is expected to strengthen China’s supervision of imported wines, help root out counterfeit products and further protect consumers’ rights. It should also guarantee quicker customs clearance procedures for imported wines     

Cao Jieming,Dedicated She Inkslab Engraver

Cao Jieming, born in 1954, learned inkslab engraving as a boy from master of the craft Wang L0sen. After 40 years of study and practice of inkslab engraving, Cao has evolved a distinctively refined style, apparent in strong but srnooth carved lines. Cao＇s representative works have won rnultiple awards at national and international exhibitions. In 2006, Cao was officially designated as national representative inheritor of the She inkstab making craft.     

Chinese Immigrants in the U.S.

IF you love her,send her to New York,for there it is heaven;if you hate her,send her to New York,for there it is hell." This telling quote from A Beijinger in New York,a famous television soap opera in the 1990s,sums up the mixed feelings that Chinese immigrants have toward the U.S. Since China opened to the world in the late 1970s,increasing numbers of Chinese people have been choosing to immigrate abroad either to study or to pursue a better life.