A simple DNA extraction method for marijuana samples used in amplified fragment length polymorphism (AFLP) analysis

As a first step in developing a molecular method for the individualization of marijuana samples, we evaluated a plant DNA extraction kit. The QIAGEN plant DNeasy method uses a spin column format for recovery of DNA and is effective for obtaining high molecular weight DNA from leaf, flower (bud), and seed samples of marijuana. The average DNA yield was 125-500 ng per 100 milligrams of fresh plant tissue. The recovered DNA was of polymerase chain reaction (PCR) quality as measured by the ability to generate reproducible amplified fragment length polymorphism (AFLP) profiles. AFLP is a technique used to create a DNA profile for plant varieties and is being applied to marijuana samples by the authors to link growers and distributors of clonal material. The QIAGEN plant DNeasy method was simple, efficient, and reproducible for processing small quantities of marijuana into DNA.     

The Participedia Project: An Introduction

ABSTRACT

Participedia is an open-source, participatory knowledge tool that responds to a new global phenomenon: the rapid development of experiments in new forms of participatory politics and governance around the world. The experiments are diverse and widespread, from the British Columbia Citizens' Assembly in Canada and the Oregon Citizens' Initiative in the United States to Participatory Budgeting in Brazil, deliberative forums in China, Panchayati Raj reforms in India, and citizen technology assessment in Denmark. Our knowledge of this rapidly expanding universe is shallow. The authors of this article, together with several others, have created the Participedia to respond to this deficit in knowledge. This article documents the development of the Participedia project to date. Participedia aims to be useful to scholars (and practitioners) as an open-source, real-time, cumulative qualitative and quantitative data repository about participatory and deliberative governance experiences. We believe that Participedia is the first effort in the social sciences to build a large data set through a method that is both crowd-sourced and structured to produce good quality, comparable information.

[Editor's Note: The authors of this article are two of the co-founders of Participedia.]     

Pussy Riot as a feminist project: Russia's gendered informal politics

This article considers Pussy Riot as a feminist project, placing their actions and the regime's reactions in the context of three post-9/11 developments in gender and sexuality politics in Russia. First, I assert that Pussy Riot's stunts are a logical reaction to the Kremlin's masculinity-based nation-rebuilding scheme, which was a cover for crude homophobic misogyny. Second, Pussy Riot is part of the informal feminism emerging in Russia, a response to nongovernmental organization (NGO) feminism and the regime's repression of NGO feminism, albeit likely to be outflanked by regime-supported thuggery. Third, the members of Pussy Riot were so harshly prosecuted because they – swearing, covered up and disloyal – violated the political cleaner role that the Kremlin has given women in the last few years. Feminist social scientists have long looked for politics outside of formal institutions and processes. The Pussy Riot affair makes clear how much gender is central to the informal politics that gender-blind observers of Russia have come to see as crucial to understanding Russia's regime.     

“She's Way Too Good to Lose”: An Evaluation of Honolulu's Girls Court

This research evaluates the efficacy of a gender-specific, problem-solving court for girl offenders. Official statistics, interview data, and focus group data are utilized to determine whether the court is achieving its stated goals of reducing recidivism, risky behaviors, and confinement for the girls who attend this court program. The present research demonstrates that the program does seem to be effective in terms of reducing both recidivism and risky behavior as well as increasing the development of prosocial and healthy relationships. The article concludes with some lessons learned from the implementation of this court program as well as ideas for future research.     

Elemental analysis of bone: proton-induced X-ray emission testing in forensic cases

Proton-induced X-ray emission (PIXE) is a spectroscopic technique that provides the researcher with the elemental composition of a given target material. In this paper, we illustrate the utility of PIXE analysis in two forensic contexts: (1) case of cremation in which the nature of the remains is questioned and (2) cases of death by gunshot wound. In the first case, elemental analysis by PIXE reveals that the purported cremated remains are not bone. The last two cases show that radiopaque metallic residue embedded in bone is composed of lead from a projectile.     

A Community‐Based Model for Remediating Juveniles Adjudicated Incompetent to Stand Trial: Feedback from Youth,Attorneys, and Judges

This study examined client satisfaction with a community‐based restoration services program for youth adjudicated incompetent to stand trial in Virginia. The sample consisted of 130 youth (ages 8‐21 years), 80 attorneys, and 43 juvenile court judges. Youth overwhelming found restoration services helpful to them, although some concepts were harder to learn than others. Both judges and attorneys were generally knowledgeable about juvenile competency law, although both were less knowledgeable about competency evaluators and the services provided to youth. Results will be used to improve teaching tools, training of Restoration Counselors, and communication between program providers and the legal community.