Mitochondrial DNA sequence alterations observed between blood and buccal cells within the same individuals having betel quid (BQ)-chewing habit

There are hundreds of millions of betel quid (BQ) lovers widely spreading around the world. Compositions in BQ may generate reactive oxygen species, which would induce DNA damage. However, oral epithelial cells as well as blood have often been used as reference samples in comparison with the mitochondrial DNA (mtDNA) sequence of hairs. The main purpose of this study was to investigate the extent of mtDNA sequence variation in regular BQ-chewers' oral epithelial cells, and thus to evaluate the forensic availability of the buccal cells from BQ-chewers using the mtDNA markers. The hypervariable segments I and II in the D-loop control region of mtDNA between paired samples of blood and buccal scrape cells from 75 non-BQ-chewers (to be a control group), 60 BQ-chewers, and 67 oral cancerous patients were DNA sequenced and compared. Among the three groups, the alteration rates of 1.3% (1 out of 75), 10% (6 out of 60), and 61% (41 out of 67) were identified from the control, BQ-chewers, and the cancerous group, respectively. In the cancerous group, as expected, high rate of DNA alteration between blood and buccal samples was found. In the BQ-chewers, one and five individuals had the length and point alterations, respectively. Interestingly, most of point alteration sites, e.g., mtDNA positions 153, 16189, 16093 identified from BQ-chewers, were also observed in previous literatures. As for the control subjects, one case with point alteration, and none with length alteration, was identified. For all the three groups, not only the oral cells but also the normal blood samples exhibited high frequency (>55%) of length heteroplasmy at poly-(C)n track. Statistical analyses revealed that significance was observed between the severity of mtDNA alteration in BQ-chewers' oral epithelial cells and the history of BQ-chewing (p = 0.02), with a tendency of positive association. Based on the guidelines by Carracedo et al., we suggest that the interpretation of mtDNA variations between criminal evidences and the oral epithelial cells (as a reference or known sample) from BQ-chewers should be performed with particular caution using the PCR-based mtDNA sequencing. Our findings would be valuable in mtDNA analysis of hair evidence, especially for those countries where the habit of BQ-chewing is popular.     

Redistricting, Candidate Entry, and the Politics of Nineteenth-Century U.S. House Elections

The effects of redistricting on candidate entry patterns in contemporary House races has received growing attention in the scholarly literature, yet virtually no consideration has been given to this question in the context of historical elections. This is unfortunate as the wider variation in congressional redistricting during the nineteenth century gives us increased leverage in terms of understanding strategic candidate behavior. Utilizing a new dataset of candidate quality for nineteenth-century House races, we examine whether candidates with prior electoral experience are more likely to run in districts that are altered during the redistricting process, and provide an account of how differences in the prevalence of redistricting may affect strategic entry decisions of politicians. Our results suggest that entry decisions and electoral outcomes are affected by redistricting in this era. Moreover, our analysis provides an opportunity to use history to test contemporary theories of congressional elections in a broader context .     

An Easy and Accurate Regression Model for Multiparty Electoral Data

Katz and King have previously proposed a statistical model formultiparty election data. They argue that ordinary least-squares(OLS) regression is inappropriate when the dependent variablemeasures the share of the vote going to each party, and theyrecommend a superior technique. Regrettably, the Katz–Kingmodel requires a high level of statistical expertise and iscomputationally demanding for more than three political parties.We offer a sophisticated yet convenient alternative that involvesseemingly unrelated regression (SUR). SUR is nearly as easyto use as OLS yet performs as well as the Katz–King modelin predicting the distribution of votes and the compositionof parliament. Moreover, it scales easily to an arbitrarilylarge number of parties. The model has been incorporated intoClarify, a statistical suite that is available free on the Internet.     

Environmental assessment of gunshot residue particles in the public domain of the United Kingdom

Only limited data currently exists on the inadvertent transfer of gunshot residue (GSR), or GSR-like particles through contact with public places. In this study, an assessment occurrence of GSR in public environments in England, UK was undertaken. Utilizing a stubbing sampling technique over 260 samples were collected from areas accessible to the public, including buses, trains, taxis, and train stations. Stub analysis was performed by Scanning Electron Microscopy with Energy Dispersive X-ray Analysis (SEM-EDX). The results showed no characteristic GSR particles were detected on any of the 262 samples taken. From these samples, a total of four indicative/consistent particles were identified on one train seat (2× BaAl, 2× PbSb). Although geographical location and firearm association is likely to influence GSR occurrence, the data suggests that the potential for inadvertent GSR transfer through contact with public transport and associated communal areas is insignificant. Further research assessing environmental background levels of GSR in additional geographical locations is critical in an evaluation of the potential for GSR transfer from the environment.     

Consistency amongst pairs: How consistent are child co-witnesses with one another?

Purpose

When multiple children are asked about the same event, the consistency of their reports may be used as a heuristic for credibility. Little research has considered how consistent child co-witnesses are likely to be. In this study, we explored how likely child co-witnesses were to report the same details from a mutually experienced event.

Methods

Pairs of children participated in an educational science event during which the target attempted to coax the children into breaking preestablished rules for the session (i.e., commit transgressions). Children were individually interviewed about their experience on two subsequent occasions.

Results

Co-witnesses tended to be quite inconsistent: 32%–55% of all details recalled were only mentioned by one co-witness. Various factors were associated with co-witness consistency, including delay before the interview, centrality of details recalled, and children's age and forthcomingness.

Conclusions

The findings indicate that inconsistency between co-witnesses reflects a natural memory phenomenon, and that practitioners should be cautious of using co-witness consistency as an indicator of credibility.     

Development of a Computer‐Assisted Forensic Radiographic Identification Method Using the Lateral Cervical and Lumbar Spine

Medical examiners and coroners (ME/C) in the United States hold statutory responsibility to identify deceased individuals who fall under their jurisdiction. The computer‐assisted decedent identification (CADI) project was designed to modify software used in diagnosis and treatment of spinal injuries into a mathematically validated tool for ME/C identification of fleshed decedents. CADI software analyzes the shapes of targeted vertebral bodies imaged in an array of standard radiographs and quantifies the likelihood that any two of the radiographs contain matching vertebral bodies. Six validation tests measured the repeatability, reliability, and sensitivity of the method, and the effects of age, sex, and number of radiographs in array composition. CADI returned a 92–100% success rate in identifying the true matching pair of vertebrae within arrays of five to 30 radiographs. Further development of CADI is expected to produce a novel identification method for use in ME/C offices that is reliable, timely, and cost‐effective.     

The wild (white) ones: comparing frames of white and black deviance

In this case study, I establish how a racial privilege shields whites from being framed as deviant by using two racially segregated motorcycle rallies as a naturalistic experiment. I conduct a content analysis of reader posts to online newspaper stories about the biker events (which regularly include deviance) and discover the bikers, their behaviors, and the proposed community reactions are framed in nearly opposite ways. Posters attribute a ‘white innocence’ to white bikers, framing them as upper class exemplars of American Individualism who temporarily suspend their morality during the rally by drinking, exposing their nude bodies, and ignoring newly passed laws, banning loud mufflers, and requiring helmets. Posters claim these unpleasant, although non-dangerous forms of deviance can be ignored, overlooked, and even celebrated as defiant acts against authority. The white innocence can appear racially innocuous until compared to the perception of black bikers who engage in similar behaviors at their rally but are framed as underclass criminals who attend the rally to steal and murder. This reveals how a usually unseen privilege shields whites from entanglements with the criminal justice system, as posters also call for further policing of racial minorities.     

The Effect of Fact‐Checking on Elites: A Field Experiment on U.S. State Legislators

Does external monitoring improve democratic performance? Fact‐checking has come to play an increasingly important role in political coverage in the United States, but some research suggests it may be ineffective at reducing public misperceptions about controversial issues. However, fact‐checking might instead help improve political discourse by increasing the reputational costs or risks of spreading misinformation for political elites. To evaluate this deterrent hypothesis, we conducted a field experiment on a diverse group of state legislators from nine U.S. states in the months before the November 2012 election. In the experiment, a randomly assigned subset of state legislators was sent a series of letters about the risks to their reputation and electoral security if they were caught making questionable statements. The legislators who were sent these letters were substantially less likely to receive a negative fact‐checking rating or to have their accuracy questioned publicly, suggesting that fact‐checking can reduce inaccuracy when it poses a salient threat.     

Literary Reviews

Tyranny and redemption. Anthills of the Savannah. Chinua Achebe. London: William Heinemann. 1987. 233pp. £10.95hb

Suffering with stoicism: Kenyan histories. Coming to Birth. Marjorie Oludhe Macgoye. London: Virago Press. 1986. 150pp. £3.95pb

The Present Moment. Marjorie Oludhe Macgoye. London: Heinemann. 1987. 155pp. £10.95hb

The politics of the soul. Search Sweet Country. B Kojo Laing. London: Picador. 1987. 318pp. £3.95pb

Images of Istanbul. The Sea‐Crossed Fisherman. Yashar Kemal. Translated from the Turkish by Thilda Kemal. London: Methuen. 1986. 289pp. £3.95pb

The Birds Have Also Gone. Yashar Kemal. Translated from the Turkish by Thilda Kemal. London: Collins Harvill. 1987. 122pp. £8.95hb

Enemies within. War in the Land of Egypt. Yusuf al Qa'id. Translated from the Arabic by Olive and Lome Kenny and Christopher Tingley. London: Al Saqi. 1986. 192pp. £4.95pb

Frustrated fantasies. Heartbreak Tango: A Serial. Manuel Puig. Translated from the Spanish by Suzanne Jill Levine London: Arean. 1987. 224pp. £2.95pb

Pubis Angelical. Manuel Puig. Translated from the Spanish by Elena Brunet. London: Faber and Faber. 1987. 236pp. £9.95hb

Tending towards one voice. Faces and Masks. Eduardo Galeano. Translated from the Spanish by Cedric Belfrage. London: Quartet. 1987. 304pp. £14.95hb

Land of riches. Cuzcatlan. Manlio Axgueta. Translated from the Spanish by Clark Hansen. London: Chatto and Windus. 1987. 255pp. £11.95hb

Autobiography of the age. The Infinite Rehearsal. Wilson Harris. London: Faber and Faber. 1987. 88pp. £8.95hb

Caribbean childhoods. Summer Lightning and Other Stories. Olive Senior. Harlow, England: Longman. 1986. 134pp. £2.95pb

Whole of a Morning Sky. Grace Nichols. London: Virago. 1987. 156pp. £3.50pb

Traumas of partition. Kingdom's End and Other Stories. Saadat Hasan Manto. Translated from the Urdu by Khalid Hasan. London/New York: Verso. 1987. 257pp. £U.95/S18.95hb

Mirroring Pakistan: reflections and distortions. The Thirteenth House. Adam Zameenzad. London: Fourth Estate. 1987. 202pp. £10.95hb

Black Mirrors. Farooq Khalid. Translated from the Urdu by Eric Cyprian. London: Jonathan Cape. 1987. 285pp. £10.95hb

Christopher Shackle. School of Oriental and African Studies, London. Prize poetry. Under Another Sky: An Anthology of Commonwealth Poetry Prize Winners. Edited by Alastair Niven. Manchester and New York: Carcanet. 1987. 103pp. £6.96/S10.95pb