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The performance of two commercial multiplex kits that together amplify the 13 core short tandem repeat (STR) loci currently in use by forensic laboratories and the U.S. national Combined DNA Indexing System (CODIS) were evaluated. The typing systems examined were AmpFlSTR Profiler Plus and AmpFlSTR COfiler (PE Applied Biosystems, Foster City, CA). Electrophoretic separation and detection of the fluorescent PCR products was achieved by capillary electrophoresis (CE) using an ABI Prism 310 Genetic Analyzer. The studies addressed the on-site validation of the instrument, the software, and each typing system. These studies included instrument sensitivity, resolution, precision, binning, peak height ratios, mixtures, stutter, and the amplification of non-probative and simulated forensic samples. Other additional developmental-type work is also reported herein, such as species specificity testing and amplification of environmentally insulted samples. Amplification conditions were found to be robust and the primer sets shown to be specific to human DNA. Stutter and peak height ratios fell within limits published by the manufacturer and other laboratories. The data demonstrate that the CE instrument can consistently resolve fragments differing in length by one base and that the +/-0.5 base bin used by the Genotyper software is acceptable for making accurate allele calls. Correct typing results were obtained from non-probative and simulated case samples, as well as samples exposed to outdoor environmental conditions. The results support the conclusion that DNA extracted from biological samples routinely encountered in the forensic laboratory can be reliably analyzed with AmpFlSTR Profiler Plus and COfiler using CE. 相似文献
113.
Case studies of the first completed Advanced Technology Program (ATP) projects have shown considerable variation in commercialization patterns of ATP-funded technologies. These variations were apparent in the timing of initial revenues, commercialization in more mature and multiple applications, and diffusion of ATP technologies relative to the period of ATP funding of R&D. This study analyzes differences in commercialization patterns for different ATP-funded technologies in a systematic way. 相似文献
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An Improved Equation for TBS and ADD: Establishing a Reliable Postmortem Interval Framework for Casework and Experimental Studies
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Colin Moffatt Ph.D Tal Simmons Ph.D. Jeanne Lynch‐Aird M.Sc. 《Journal of forensic sciences》2016,61(Z1):S201-S207
Megyesi et al.'s (J Forensic Sci, 2005, 50, 618) paper was important to forensic anthropology as it introduced a quantitative framework for estimating time since death in human cadavers, based upon physical appearance by way of scoring on a novel scale. However, errors concerning rounding, temperature scale, and incorrect use of a statistical regression model render their predictive formula unusable. Based upon only their more reliable data, a more appropriate regression model to predict accumulated degree days (ADD) from total body score (TBS) is presented. The new model is also a superior fit (r2 = 0.91) and produces markedly narrower confidence intervals than the original, which also allowed impossible, negative ADD values. Explanations of the shortcomings in the original analysis and calculations are presented, which it is hoped will help forensic scientists avoid making similar mistakes. 相似文献
116.
To what extent can public enterprises serve as instruments ofpolicy enabling the federal government to assert a measure ofcontrol over Canada's foreign economic relations? This articlepresents an overview of public enterprises in Canada and highlightsthe special features of the Canadian political economy whichdifferentiate it from other advanced industrial economies. Theactivities of the Canada Development Corporation and CanadianNational Railways are analyzed in terms of whether they reinforceor undermine the federal government's authority in foreign economicpolicymaking. It appears that the relative autonomy of publicenterprises from government controls, most pronounced in thecase of commercial enterprises operating in competitive markets,fragments decisionmaking. Given that governmental power in Canadais already fragmented due to competitive federalism, and giventhe expanded activities of provincial public enterprises inrecent years, a lack of direction over the "subgovernment" offederal public enterprises compounds the difficulties for Ottawato conduct a coherent, foreign economic policy. 相似文献
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The effectiveness of social skills training (SST) for juvenile delinquents: a meta-analytical review
van der Stouwe Trudy Gubbels Jeanne Castenmiller Yvonne L. van der Zouwen Marion Asscher Jessica J. Hoeve Machteld van der Laan Peter H. Stams Geert Jan J. M. 《Journal of Experimental Criminology》2021,17(3):369-396
Journal of Experimental Criminology - To examine the effectiveness of social skills training (SST) for juvenile offenders and for whom and under which conditions SSTs are the most effective.... 相似文献
119.
Ian Lundberg Sarah L. Gold Louis Donnelly Jeanne Brooks‐Gunn Sara S. McLanahan 《Journal of policy analysis and management》2021,40(1):107-127
A lack of affordable housing is a pressing issue for many low‐income American families and can lead to eviction from their homes. Housing assistance programs to address this problem include public housing and other assistance, including vouchers, through which a government agency offsets the cost of private market housing. This paper assesses whether the receipt of either category of assistance reduces the probability that a family will be evicted from their home in the subsequent six years. Because no randomized trial has assessed these effects, we use observational data and formalize the conditions under which a causal interpretation is warranted. Families living in public housing experience less eviction conditional on pre‐treatment variables. We argue that this evidence points toward a causal conclusion that assistance, particularly public housing, protects families from eviction. 相似文献
120.
This article introduces a new explanation for why citizens may fail to vote based on government performance. We argue that when politicians have limited capacity to control bureaucrats, citizens will not know whether government performance is a good signal of the incumbent's quality. We develop a selection model of elections in which policy is jointly determined by a politician and a bureaucrat. When politicians have incomplete power over policy, elections perform worse at separating good and bad types of incumbents. We test the theory's predictions using survey experiments conducted with nearly 9,000 citizens and local officials in Uganda. We find that citizens and officials allocate more responsibility to politicians when they are perceived as having more power relative to bureaucrats. The allocation of responsibility has electoral consequences: When respondents believe that bureaucrats are responsible for performance, they are less likely to expect that government performance will affect incumbent vote share. 相似文献