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A validation study for the extraction and analysis of DNA from human nail material and its application to forensic casework. 总被引:5,自引:0,他引:5
T D Anderson J P Ross R K Roby D A Lee M M Holland 《Journal of forensic sciences》1999,44(5):1053-1056
A validation study was conducted to demonstrate that deoxyribonucleic acid (DNA) could be successfully extracted from human nail material and analyzed using short tandem repeat (STR) profiling and/or mitochondrial DNA (mtDNA) sequencing. This study involved the development of a DNA extraction protocol that includes a cleaning procedure designed to remove external contaminants (e.g., biological, chemical). This protocol was used to test human nail material that had been soaked in whole blood from a second donor and coated with gold-palladium to simulate scanning electron microscopic analysis. The results showed no indication of a mixture and were consistent with that of the nail donor. Fresh human nail material usually yielded both STR profiles and mtDNA sequence information; however, aged human nail material (approximately eight years old) yielded only mtDNA sequence information. Upon completion of the validation study, the extraction protocol was used for the analysis of a torn fingernail fragment recovered from the scene of a violent homicide in 1983. A partial STR profile and mtDNA sequence information indicated that the fingernail fragment was excluded as originating from the suspect and was, in fact, consistent with originating from one of the victims. 相似文献
184.
Law and Human Behavior - 相似文献
185.
The relationship between psychosocial maturity (psychological health) and assertiveness was investigated in a sample of United States college males and females. Results revealed a moderately high positive relationship between psychosocial maturity (PSM) and self-reported assertiveness on the Rathus and Galassi scales for both sexes. This relationship was slightly stronger (in terms of variance accounted for) for males than females, significant differences being obtained for Intimacy on the Rathus scale and PSM and Intimacy on the Galassi scale. Multiple regression analyses revealed that the personality components most consistently accounting for major portions of the variance in predicting male assertiveness scores on both the Rathus Assertiveness Schedule and the College Self-Expression Scale were Intimacy and Initiative, while in predicting female assertiveness, only Initiative was involved. The findings were related to previous research, recent work on the androgyny construct (instrumental vs. expressive behaviors), and exhortations for increased cooperation between schools of psychotherapy to establish it as a more unified discipline.Received his Ph.D. from Syracuse University in 1971. He has also been a Post-Doctoral Fellow in Clinical-Community Psychology at the University of South Carolina and a Clinical Psychologist at the Behavioral Medicine Unit of St. Joseph's Hospital- McMaster University in Hamilton, Ontario, Canada. Current research interests include personality moderators of social cognition and self-concept theory.Received his Ph.D. in Psychology from Northern Illinois University in 1972. Current research interests include personality moderators of social cognition and behavior change, and psychological assessment. 相似文献
186.
Doreen A. Rosenthal Ross M. Gurney Susan M. Moore 《Journal of youth and adolescence》1981,10(6):525-537
A new inventory for examining the first six of Erikson's psychosocial stages is described. The self-report questionnaire, developed in a pilot study of 97 adolescents and tested in a study of 622 adolescents, has 12 items for each subscale. Measures of reliability and validity are reported. It is concluded that the Erikson Psychosocial Stage Inventory (EPSI) is a useful measure for researchers interested in development from early adolescence and in mapping changes as a function of life events.This research was supported by a grant from the Education Research and Development Committee.Received Ph.D. from University of Melbourne. Current research interests are adolescent adjustment, ethnicity, and sex-role psychology.Received Ph.D. from University of Melbourne. Current research interests are effects of unemployment and adolescent adjustment.Received Ph.D. from Florida State University. Current research interests are sex-role psychology and adolescence. 相似文献
187.
This study examined the perceived social, behavioral, and health effects of phencyclidine (PCP) use among a sample of 200 users in northern California. The study population, generated through snowball sampling techniques, included heavy chronic, light chronic, and recreational users; the vast majority of subjects were not in treatment facilities. Subjects reported that PCP use—particularly long-term, extensive use—resulted in frequent disruption of education and employment, impairment of relationships with parents and with lovers or spouses, involvement with the criminal justice system, and deterioration of physical and mental health. These findings provide systematic documentation of the extent to which problems previously associated with PCP use occurred among this population of users. In addition, the results illustrate the importance of examining the effects of PCP use in light of the psychosocial developmental processes of adolescence and early adulthood.This research was supported by Research Grant No. DA02293 from the National Institute on Drug Abuse.Received M.S. in psychology from California State University, Hayward.Received Ph.D. from University of Iowa. 相似文献
188.
189.
Ian Leigh 《The Modern law review》1999,62(2):298-309
Book reviewed in this article:
Adam Tomkins, The Constitution after Scott: Government Unwrapped 相似文献
Adam Tomkins, The Constitution after Scott: Government Unwrapped 相似文献
190.