Enhanced elution of sperm from cotton swabs via enzymatic digestion for rape kit analysis

This report describes development of a method for enhanced cell elution from cotton swabs. The method exploits an enzyme mixture for digestion of the cotton to remove intact cells, and can be utilized in conjunction with or to circumvent conventional differential extraction (DE). Samples digested with Aspergillus niger cellulase yielded sperm cell recoveries (18+/-3.5%) similar to conventional DE buffer (23+/-7.8%) while providing intact epithelial cells. Storage time affected the concentration of enzyme required for optimal sperm cell recovery, with longer times requiring increased cellulase concentrations. Cellulase from A. niger yielded a twofold enhancement in sperm cell elution over buffer alone, and preliminary testing of higher activity cellulases from Trichoderma reesei and Trichoderma viride showed even greater enhancement. These results indicate that cellulose-digesting enzymes enhance the release of sperm and epithelial cells from a cotton swab over buffer alone, providing for efficient DNA analysis.     

Gender and International Relations: A Global Perspective and Issues for the Caribbean

In this paper we discuss the relatively recent integration of feminist thinking in the discipline of International Relations. We argue that the theoretical foundations of International Relations are still primarily based on traditional male-female dichotomies, particularly that of separate public and private spheres. By extension, women are largely excluded from state power and decision making. The state is itself gendered. The growing recognition of the links between the global economy and gender forces us to engage with International Relations in foreign and international policy. In this article we look at feminist interpretations of three main International Relations areas: international security, human rights, and international political economy and their implications for gender policies in the Caribbean. We also look at the contributions of Caribbean women to the international feminist agenda and suggest a research agenda for ongoing feminist theorizing in the discipline of International Relations.     

An Update on the Hazards and Risks of Forensic Anthropology,Part I: Human Remains

This work reviews the hazards and risks of practicing forensic anthropology in North America, with a focus on pathogens encountered through contact with unpreserved human remains. Since the publication of Galloway and Snodgrass' seminal paper concerning the hazards of forensic anthropology, research has provided new information about known pathogen hazards, and regulating authorities have updated recommendations for the recognition and treatment of several infections. Additionally, forensic anthropology has gained popularity, exposing an increased number of students and practitioners to these hazards. Current data suggest many occupational exposures to blood or body fluids go unreported, especially among students, highlighting the need for this discussion. For each pathogen and associated disease, this work addresses important history, reviews routes of exposure, provides an overview of symptoms and treatments, lists decontamination procedures, and presents data on postmortem viability. Personal protection and laboratory guidelines should be established and enforced in conjunction with the consideration of these data.     

Predictions put into practice: a quasi-experimental evaluation of Chicago’s predictive policing pilot

Objectives

In 2013, the Chicago Police Department conducted a pilot of a predictive policing program designed to reduce gun violence. The program included development of a Strategic Subjects List (SSL) of people estimated to be at highest risk of gun violence who were then referred to local police commanders for a preventive intervention. The purpose of this study is to identify the impact of the pilot on individual- and city-level gun violence, and to test possible drivers of results.

Methods

The SSL consisted of 426 people estimated to be at highest risk of gun violence. We used ARIMA models to estimate impacts on city-level homicide trends, and propensity score matching to estimate the effects of being placed on the list on five measures related to gun violence. A mediation analysis and interviews with police leadership and COMPSTAT meeting observations help understand what is driving results.

Results

Individuals on the SSL are not more or less likely to become a victim of a homicide or shooting than the comparison group, and this is further supported by city-level analysis. The treated group is more likely to be arrested for a shooting.

Conclusions

It is not clear how the predictions should be used in the field. One potential reason why being placed on the list resulted in an increased chance of being arrested for a shooting is that some officers may have used the list as leads to closing shooting cases. The results provide for a discussion about the future of individual-based predictive policing programs.

     

1‐800‐Skype‐Me

It is increasingly common that children of divorce are geographically separated from one of their parents. This article considers the challenges that arise from that reality by exploring this problem from a variety of perspectives and by providing practical tips to minimize the impact of the distance. A review of the Ontario caselaw and Arizona Guidelines reveal that certain factors are important in the resolution of these disputes, including: the age of the child, mode of transportation between homes, distance, prior contact, and feasibility of virtual access. Court‐ordered access may include remedies that, absent the distance issue, may be considered extreme, including moving to overnight/extended access periods for young children, permitting children to travel unaccompanied, favoring the nonresident parent for holidays and vacation time, allowing children to decrease contact with the nonresident parent, and decreasing or terminating child support. Where distance dictates the in‐person and virtual access schedules, creative solutions are critical to the successful resolution of these cases. Forward thinking family law professionals can meaningfully help parents to achieve better outcomes for children.     

The effectiveness of social skills training (SST) for juvenile delinquents: a meta-analytical review

Journal of Experimental Criminology - To examine the effectiveness of social skills training (SST) for juvenile offenders and for whom and under which conditions SSTs are the most effective....     

Putting More Public in Policy Analysis

A persistent criticism of policy analysis is that it undermines basic democratic institutions and processes by replacing public participation with expert analysis. Many decision makers shun broader participation because of the complexity of an issue or the cost, uncertainty, and delay often associated with public involvement. This article presents a model for the systematic inclusion of public input into relatively complex public policy decisions. It outlines two determinants of success in public participation efforts: the purpose for public involvement and the nature of the issue; furthermore, it applies the model to two issues in recent Utah history that have involved public participation. Using these principles, decision makers should be able to design and implement public participation strategies that both inform the public about substantive policy questions and improve the quality of the final decision.