Anion Identification via Complexation with meso‐octamethylcalix(4)pyrrole and Detection Using Electrospray Ionization Mass Spectrometry*

Abstract: The routine identification of controlled substances and adulterants during forensic chemistry analysis often involves the identification of counter ions or salt forms present in an exhibit. Here, the use of the compound meso‐octamethylcalix(4)pyrrole (C4P) during salt‐form identification analysis is presented. C4P is a commercially‐available, anion‐binding agent that can be reacted with a controlled substance or adulterant, resulting in the sequestration of anionic species, usually present as counter ions to the active ingredient. Formation of noncovalent complexes between the cyclic host C4P compound and anionic guests is investigated using electrospray ionization–mass spectrometry (ESI–MS). Complexes with chloride, bromide, iodide, nitrate, and acetate are readily observed and mass spectrometry analysis provides identification via molecular weight characterization. Chloride and bromide complexes are also characterized by the isotopic distribution of their molecular ions. Formation of host–guest complexes is not observed for sulfate and phosphate salts, presumably due to steric hindrance and energetically unfavorable conditions.     

Analysis of forensic samples in Banco Nacional de Datos Genéticos

Analysis of forensic samples to evaluate the rate of success for molecular markers: autosomal STRs, Y chromosome, and mitochondrial DNA. Since 2006 to date a total of 390 forensic samples were analyzed: bones, teeth, hairs, swabs, stains and paraffin embedded tissue. Bones and teeth, were pulverized in a Freezer Mill, extracted by chloroform/phenol/isoamyl alcohol method, and then purified with Centricon 100 columns. DNA from paraffin was extracted with QIAmp DNA Mini kit (QIAGEN). Mitochondrial DNA Control Region sequences were determined for regions HV1/HV2. Sequencing was performed using the BigDye^® Terminator v 1.1 Kit and analyzed in ABIPRISM^® 3100 Genetic Analyzer (AB). STRs were amplified using Amp FlSTR Identifiler^®, Minifiler^® and YFiler^® Kit (AB) and analyzed in ABI PRISM^® 3100 Genetic Analyzer and ABI PRISM^® 3130xl Genetic Analyzer (AB). Among forensic samples, bones and teeth analyzed for autosomal STRs, we obtained successful results in all of them. Incomplete typing are represented by loci of higher molecular weight, which demonstrates the poor quality of the sample due to its state of degradation and obtained better results using mini STRs. Successful results in sequencing for mitochondrial HV1 region for all samples analyzed, but in few hair samples we obtained mixed sequences and that represented important difficulties for the analysis. Age of samples and conservation are factors related which affect DNA viability. Autosomal STRs solved all the samples analyzed in our study, but Y chromosome analysis and mitochondrial DNA sequencing are also important and necessary markers in some forensic cases.     

A Peruvian population study of eight Y-chromosome STR loci

We have analyzed the distribution of the allele frequencies and haplotypes at eight Y-chromosomal short tandem repeat (STR) loci (DYS437, DYS438, DYS439, DYS460, DYS461, GATA A10, GATA C4 and GATA H4) in a sample population of 87 unrelated individuals from Perú.     

Genetic profile of a multi-ethnic population from Guiné-Bissau (west African coast) using the new PowerPlex 16 System kit

Allele and haplotype frequencies of 15 chromosome STR loci included in the kit PowerPlex16 System from Promega, were determined in a sample of unrelated males from Guiné-Bissau, a country from the west African coast. All individuals were subjected to an interview in order to make sure that their ancestors belonged to the same ethnic group. This way we intended to look for possible inter-ethnic differences. PowerPlex 16 includes STRs not studied before in any multi-ethnic population. The kit includes two new allele markers (Penta D and Penta E), which are very useful either in forensics or population genetic studies. The Guinean population presents significant differences when compared with other African populations.     

Internalizing Symptoms in Latinos: The Role of Anxiety Sensitivity

Latin American youth in the United States tend to report more internalizing symptoms than white non-Latino youth, yet little is known about the factors that may contribute to such differences. The present study examined the role that anxiety sensitivity, gender, and ethnic minority status may play in the expression of internalizing symptoms across Latin American adolescents (n = 116) and white non-Latino adolescents (n = 72) in the United States and Colombian adolescents in Colombia (n = 163). Results provide evidence that because fear of anxiety related phenomena and physiological symptoms of anxiety in particular may be normative in Latino culture anxiety sensitivity does not amplify somatic complaints for Latin American and Colombian youth as it does for white non-Latino youth. Results further suggest that anxiety sensitivity and being female predicted anxiety and depressive symptoms independent of cultural background. Implications of the findings to our understanding of cultural variability in internalizing symptoms are discussed. R. Enrique Varela, PhD, is an assistant professor of psychology at Tulane University. He received his PhD from the University of Kansas Clinical Child Psychology Program. His research interests are cross cultural manifestations of childhood anxiety and parenting practices in Latin American families. He is also interested in adherence issues in chronically ill children. Carl F. Weems, PhD, is an associate professor of psychology at the University of New Orleans. He received his PhD from Florida International University and did post doctoral work at Stanford Medical School. His research focuses on the developmental psychopathology of anxiety and depression. In particular, his research integrates developmental, cognitive, biological and behavioral theories in attempting to understand the etiology and course of internalizing disorders in childhood. Special areas of interest include the assessment and treatment of childhood anxiety disorders, the role of cognitive behavioral development, brain function, and cognitive processing in anxiety and depression. Steven L. Berman, PhD, is an assistant professor of psychology at the University of Central Florida. He received his PhD from Florida International University. His research interests are identity development including associated anxiety and distress, cross-national comparisons, and the development of identity interventions. Lauren Hensley, MS, is a graduate student in psychology at Tulane University. Her main research interest is anxiety development, with a focus on anxiety sensitivity and children’s responses to traumatic events. Maria Clara Rodriguez de Bernal, MS, is an assistant professor of psychology at Universidad de la Sabana, Bogota, Colombia. Her research interests are in the area of program evaluation dealing with anxiety disorders, posttraumatic stress disorder in particular.     

Measuring Quality of Life in Spanish Municipalities

Abstract

Measuring quality of life in municipalities entails two empirical challenges. First, collecting a set of relevant indicators that can be compared across the municipalities in the sample. Secondly, using an appropriate aggregating tool in order to construct a synthetic index. This paper measures quality of life for the largest 237 Spanish municipalities using Value Efficiency Analysis (VEA) to derive comparative scores by combining the information contained in 19 partial indicators. VEA is a refinement of DEA (Data Envelopment Analysis) that imposes some consistency in the weights of the indicators used to construct the aggregate index. The indicators cover aspects related to consumption, social services, housing, transport, environment, labour market, health, culture and leisure, education and security. The results show that the Northern and Central regions in Spain attain the highest levels of quality of life, while the Southern regions report low living conditions. Education is the variable that requires the largest improvement in low performing municipalities, followed by health and culture facilities, pollution and crime. Population density, growth and ageing seem to relate positively to quality of life.     

Do Formalist Judges Abide By Their Abstract Principles? A Two-Country Study in Adjudication

International Journal for the Semiotics of Law - Revue internationale de Sémiotique juridique - Recent literature in experimental philosophy has postulated the existence of the...     

Faith,Folie à Famille,and Mummification: A Brief Review of the Literature and a Rare Case Report

Folie à deux is a rare clinical syndrome characterized by the transference of delusional ideas from one person to one or more other people in close association with the primary affected patient. Mummification indicates the preservation of the corpse of a person for a variable period of time. A brief review of the literature in this field is presented, and an exceptional case is described, characterized by the association of both these rare phenomena. The case is an example of folie à fammille which developed out of a condition of extreme religiousness and seclusion of an entire family. The shared psychosis led to the horrible death of some of the family members, while the last surviving member of the family lived for more than a year and a half with their mummified remains. The Judge commissioned a forensic psychiatry assessment to verify the survivor's ability to bear witness. The development of the psychiatric syndrome and its consequences are extensively discussed.     

Psychological and behavioral adjustment in female youths with high or low psychopathic traits

The aim of the present study was to analyze the role of psychopathic traits in female juvenile delinquency. Using a sample of 236 young females from the Juvenile Detention Centers of the Portuguese Ministry of Justice and schools in the Lisbon area, a group of female youths with high psychopathic traits (n = 118; M = 15.84 years of age; range = 14–18 years of age) and a group of female youths with low psychopathic traits (n = 118; M = 15.77 years of age; range = 14–18 years of age) were formed based on the Portuguese version of the Antisocial Process Screening Device-Self-report (APSD-SR). The results showed that young females with high psychopathic traits start engaging in criminal activities and come into contact with the justice system earlier in life; exhibit higher levels of behavioral problems, conduct disorder, delinquent behaviors and serious criminality; and demonstrate lower levels of self-esteem and pro-social behavior. The importance of some variables in predicting group membership (high versus low psychopathic traits) was established through a binary logistic regression. Our findings reinforce the importance of the psychopathy construct for the early identification of potentially high-risk female youths and for the assessment of female youths who have already come into contact with the judicial system.     

Family Dysfunction and Social Isolation as Moderators Between Stress and Child Physical Abuse Risk

Stress is a significant contributor to child physical maltreatment risk. Family and social supports are expected, but less studied, risk factors. Little empirical support clarifies the interactive influence on abuse risk for non-abusive parents. This study examined whether the stress-abuse risk relation was moderated by family dysfunction and social isolation. Subjective appraisals of these factors were administered to 95 community mothers. After creating composite scores using factor loadings from a CFA, multiple regression analyses were conducted to predict abuse risk. As expected, stress predicted abuse risk, with social isolation and, to a lesser extent, family dysfunction serving as moderators. Perceived stress and dysfunctional supports are important, interactive predictors of abuse risk. Future directions consider interactions within other ecological levels.