猪瘟病毒E2蛋白B细胞识别基序的筛选

根据猪瘟病毒(classical swine fever virus,CSFV)E2蛋白的序列设计并人工合成18条重叠多肽,与载体蛋白BSA偶联后,利用抗CSFV E2蛋白单克隆抗体SWmAb8筛选该蛋白上的B细胞表位;同时用12肽噬菌体随机肽库淘选E2单抗识别多肽序列。结果显示,多肽序列VSPTTLRTEV能被单抗SWmAb8识别;含SPTxLR基序的噬菌体能被单抗SWmAb8结合,且能被病毒抗原竞争性抑制。证实SPTxLR基序很可能是E2蛋白的线性表位之一,可诱导机体产生中和抗体。     

完善公路投融资体制的几点思考

本文分析了我国改革开放以来公路建设投融资方面存在的问题,探讨了完善公路建设投融资体制的若干对策建议。     

法律论证的几个基本理论问题

一、法律论证理论的兴起 法律论证是近三四十年来在欧美学界兴起并且获得重要发展、取得重要地位的研究领域.一般认为,直到1970年,法律论证还主要是在法律理论(Rechtstheorie或"法理论")和法哲学的语境下进行研究.[1]"法律论证被当作法律逻辑亦即法律方法论或法律判决制作理论的一部分,而非本来意义上的法律论证理论."[2]随着律师和论证理论家们对法律论证的兴趣日趋高涨.法律论证不再仅仅被当作一种比较宽泛的研究领域,而是被作为一项本来意义上的研究课题.对法律判决进行理性证立的问题,已经成为处于不断演进中的法律论证理论的核心主题.     

Developmental validation of the Microreader™ Y Prime Plus ID System: An advanced Y-STR 38-plex system for forensic applications

Y-STR is widely used in sexual assaults and familial searches of suspects. Here, we reported a novel 38-plex STR genotyping system designed for forensic applications. Microreader? Y Prime Plus ID System (YPP) amplifies 38 loci in one reaction, including 29 loci from commonly used Yfiler® Plus PCR Amplification Kit & PowerPlex® Y23 System (DYS393, DYS570, DYS19, DYS392, DYS549, Y GATA H4, DYS460, DYS458, DYS481, DYS635, DYS448, DYS533, DYS449, DYS456, DYS389I, DYS390, DYS389Ⅱ, DYS438, DYS391, DYS439, DYS437, DYS385a/b, DYS643, DYS518, DYS576, DYF387S1a/b, and DYS627), 6 commonly used loci for the Y-STR database (DYS444, DYS447, DYS596, DYF404a/b, DYS527a/b, DYS557) and one Y-indel specific for the Chinese population. YPP is designed for different types of samples, such as blood card and swabs. In this work, YPP was validated following SWGDAM guidelines (2016) and guidelines from Ministry of Public Security of the People’s Republic of China, including PCR-based, sensitivity, accuracy and precision, mixture, stability and inhibitor, and species specificity. The results indicate that the Microreader? Y Prime Plus ID System is a powerful identification kit designed for forensic databases.     

Identification of Natural Images and Computer‐Generated Graphics Based on Statistical and Textural Features

To discriminate the acquisition pipelines of digital images, a novel scheme for the identification of natural images and computer‐generated graphics is proposed based on statistical and textural features. First, the differences between them are investigated from the view of statistics and texture, and 31 dimensions of feature are acquired for identification. Then, LIBSVM is used for the classification. Finally, the experimental results are presented. The results show that it can achieve an identification accuracy of 97.89% for computer‐generated graphics, and an identification accuracy of 97.75% for natural images. The analyses also demonstrate the proposed method has excellent performance, compared with some existing methods based only on statistical features or other features. The method has a great potential to be implemented for the identification of natural images and computer‐generated graphics.     

在市委党校（行政学院）2011年春季主体班开学典礼上的讲话

同志们：今天,我们在这里举行2011年春季市委党校（行政学院）县级干部进修班、中青年后备干部培训班和党外干部培训班开学典礼。在此,我代表市委,向参加培训班的各位学员表示热烈的欢迎!向辛勤工作在干部教育培训战线上的党校（行政学院）教职员工致以诚挚的问候!     

快速出枪和快速射击的教学训练模式及其难点分析

快速出枪和快速射击是警察查缉战术的重要组成部分,目前该技术已在全国公安系统内推广普及,本文根据自己十年的教学研究,归纳总结出一整套快速出枪和快速射击的具体技术理论、教学训练方法及难点、重点、考核标准和方法,以促进该技术在公安基层一线的推广和普及.     

突破中俄能源企业合作“瓶颈”的对策分析

中国与俄罗斯地理相邻,需求与供应互补,能源合作空间非常广阔。自20世纪90年代起,中俄能源领域便开始合作,但多年来,合作步伐非常缓慢,其主要原因在于缺少中俄能源企业间的合作。在市场经济条件下,企业是经济活动的主体,是经济社会中最活跃的因素,是推动经济发展的重要力量。中俄能源企业合作的水平决定了中俄能源合作的深度。中俄能源企业合作是深化中俄能源合作的重要途径。在中俄能源企业合作的进程中有成绩,也存在问题。突破能源企业合作的"瓶颈",是实现中国与俄罗斯在能源领域更深层次合作的关键所在。     

On disclosure policy in contests with stochastic entry

We study how a contest organizer who seeks to maximize participant effort should disclose the information on the actual number of contestants in an imperfectly discriminatory contest with stochastic entry. When each potential contestant has a fixed probability of entering the contest, the optimal disclosure policy depends crucially on the properties of the characteristic function H(?)=f(?)/f ^??(?), where f(?) is the impact function. The contest organizer prefers full disclosure (full concealment) if H(?) is strictly concave (strictly convex). However, the expected equilibrium effort is independent of the prevailing information disclosure policy if a linear H(?) (Tullock Contest) applies.     

用社会主义核心价值观引领青春文学潮

青春文学是本世纪初新兴的文化思潮.本文试图通过对青春文学三大特异性分析,阐释社会主义核心价值观对于青春文学生成发展的特殊功能意义.探讨用社会主义核心价值观引领青春文学潮的方法途径.