胶体金标记苯丙胺单克隆抗体免疫试剂盒研究

目的建立一种准确、快速、简便的检测尿液中苯丙胺(AMP)的胶体金免疫层析技术。方法采用柠檬酸三钠还原法制备胶体金颗粒,标记抗AMP单抗,将AMP—BSA抗原固相于硝酸纤维素膜上,制备胶体金免疫层析测试条。通过尿液、血液和唾液中的可能存在的苯丙胺成分与测试条上的苯丙胺-BSA完全抗原竞争结合有限的单抗结合位点,来判定检测结果。结果用ICT法和GC/MS检测217份尿样,本法检测阈值为1000ng/mL,特异性为99.17%,准确性为99.54%。结论ICT法检测尿液中的AMP特异性强,灵敏度高、简便快速、无需特殊仪器设备,具有广泛应用价值。     

Four Lessons for Developing Countries from the Trade Negotiations Over Access to Medicines

After the Agreement on the Trade-Related Aspects of Intellectual Property Rights (TRIPS) came into operation in 1995 developing countries have found themselves in a process of continual negotiation over intellectual property rights and access to medicines. These negotiations have taken place in the World Trade Organization and in the context of free trade agreements. The paper suggests that the only real win for developing countries has been the Doha Declaration on the TRIPS Agreement and Public Health in 2001. What have been the lessons for developing countries in a decade of negotiations over access to medicines? Drawing on themes of rule complexity and regulatory ritualism the paper discusses four key lessons for developing countries. It concludes by arguing that developing countries will do better if they adopt a networked governance approach to negotiation rather than continuing to rely on traditional coalition formation.     

试析冷战后印度与东盟的关系

近年,从前任总理瓦杰帕伊突破禁忌,打开高层对话开始,印度与东盟的关系开始不断地升温。从经济层面不断地向政治、军事、安全和防务等高层次发展,借此印度的经济持续快速发展,在东盟的政治影响也不断扩大。这种全面合作的加强正体现了印度的务实外交,把20世纪90年代拉奥总理的“东向政策”发展到一个新的台阶。一、冷战后印度与东盟关系的进展历史上,印度与东南亚地区一直有着十分密切的联系。从佛教的传播,到大批旅居东南亚的印度裔,从穿行两地的商贾船队到飘香东南亚的印度咖喱,印度政治、宗教、文化对东南亚有着潜移默化的影响。但后来相…     

朊毒体蛋白的构象及其特性的研究进展

概述了朊毒体(包括构象不同的细胞型朊毒体和痒病型朊毒体)蛋白的构象特征、朊毒体及其突变体的特性,阐明了PrPsc是由PrPc通过构象转变而成的,不同物种PrP的三维结构有相似的球形结构域(125~228位)和N-末端无规卷曲尾,其球形结构域均由3个螺旋(144~154、173~194和200~228位)以及1对反平行的β-折叠(128~131和161~164)组成,但其结构和表面电荷分布存在一些区别。     

“The Spectacle of Fearsome Acts”: Crime in the Melting P(l)ot in <Emphasis Type="Italic">Gangs of New York</Emphasis>

This paper explores the multi-layered representations of violence and crime in the recent Hollywood film Gangs of New York [Scorcese (2003) Miramax]. We use our exploration of this film to suggest that popular culture, even through its most mainstream products, can be seen as a critical criminological space where alternative views of law, crime and the state are made available. Rather than understanding Hollywood movies simply as vehicles for disseminating conventional mores, we suggest that they can furnish critical (and complex) points of view on law and crime and that the project of a critical criminology can be strengthened by engaging more forthrightly with these ubiquitous cultural forms.     

当前推行行政执法责任制中存在的主要问题及对策研究

近年来各级人民政府、各行政执法部门广泛建立了行政执法责任制,对规范行政行为、提高执法质量、维护群众权益起到了积极的作用,但在执行中也暴露出一些问题。本文分析了一些突出问题的深层次原因,并对顺利推行行政执法责任制提出对策建议。     

人权的国际保护与不干涉内政

<正> 人权,本是国内法的一个概念,是作为一个法律原则和公民权利被规定在有关国家的宪法、民法或其他法律之中.第一次世界大战后,某些国际条约开始出现有关保护少数条款,第二次世界大战后,人权问题大规模地进入国际法的领域.目前,根据国际上较为普遍的意见,人权既包括个人人权,也包括集体人权,即民族自决权和发展权等.但是,由于国际人权文书具有不同的法律性质,特别是由于各国社会政治制度、意识形态、价值观念以及民族传统的不同,有关人权的解释和运用,是不同的.再加上一些国家将人权作为推行其对外政策、     

多媒体教学在本科民事诉讼法学课程中的应用

多媒体教学及其效果已成为国家评价教学质量的重要指标。具体到法学本科教学,我们应当注重多媒体教学的应用,为保证和提高法学本科课程教学效果与质量建立完善的教学方法体系。我们在总结多媒体在本科民事诉讼法学课程教学中应用的理论和实践经验的基础上,探究多媒体应用于本科民事诉讼法学课程教学的方式以及应当注意的问题。     

The challenge of institution-building in Asia and its implications for Asia-Europe relations

  East Asia, including Northeast Asia and Southeast Asia, has developed tightly-linked production/distribution networks through globalizing corporate activities. The vertical chain of production in East Asia has been even more sophisticated than economic integration in East Europe or Latin America. However, the political environment of East Asia for trade and investment has been far from borderless. The integration effort at the policy level has been very much limited so far, due to the historical background as well as geopolitics surrounding East Asia. The Asian currency/financial crisis provided these countries a historical turning point. After the burst of the crisis, East Asians realized that they have to take care of themselves in their difficulties, not depending on outside forces. A natural choice for them was to step into the realm of regionalism. In 1998, Japan and Korea officially announced that they would discard the long-lasting GATT/WTO-only approach and adapt the multi-layered approach, including both regionalism and multilateralism. The ultimate goal of regionalism would be a region-wide integration including ASEAN+3. As a steppingstone, Japan signed the Japan-Singapore Economic Partnership Agreement (JSEPA) in January 2002. In a parallel move, the ASEAN and China Leaders announced in November 2001 the establishment of an ASEAN-China Free Trade Area (ACFTA) within 10 years. This article will follow up the most recent advancement of regional institutional building in East Asia with the emphasis on peculiar characteristics of economic integration in the region and discuss its implications for Asia-Europe relations. This paper is heavily drawn from Kimura (2002, 2003).     

鉴定人的责任负担

鉴定人是司法鉴定活动的决定性因素。鼓励专家参与法庭服务与约束专家独立、客观实施鉴定这一对矛盾需要通过制度的合理设置达到一定的平衡状态,以适合诉讼活动的开展。鉴定人的责任负担是体现对鉴定人实施鉴定活动进行约束的主要手段,也是我国进一步深化司法鉴定体制改革不可回避的重要内容之一。