Determination of blood/serum ratios of different forensically relevant analytes in authentic samples

For forensic toxicological investigations only whole blood, but no serum is often available. Pharmacokinetic data are helpful for interpreting the results, but most of these studies indicate serum or plasma concentrations. In order to obtain reliable conversion factors which also take intersubject variability into account, the blood/serum ratios (B/S) of oxycodone, morphine, fentanyl, hydromorphone, zopiclone, MDMA, dexamphetamine, alprazolam, risperidone and 9-hydroxyrisperidone were determined by LC-MS/MS using authentic samples. Blood and corresponding serum samples were obtained from driving studies performed with controlled or known dosages of the above drugs. The analytes were analysed in blood and serum and the following mean B/S ratios (relative standard deviations) were determined: oxycodone 1.48 (8.19 %); morphine 1.03 (3.59 %); fentanyl 0.87 (13.9 %); hydromorphone 1.04 (8.11 %); zopiclone 0.89 (16.1 %); MDMA 1.19 (8.04 %); dexamphetamine 0.89 (10.9 %); alprazolam 0.81 (5.84 %); risperidone 0.65 (7.52 %); 9-hydroxyrisperidone 0.73 (12.3 %). These mean values are largely in line with those reported in the literature. The B/S ratios did not appear to depend on partition coefficients, whereas there was strong evidence that B/S ratios decreased with increasing plasma protein binding.     

Medical and legal aspects of genital mutilation and circumcision part I: female genital mutilation (FGM)

Female genital mutilation (FGM) is considered to be against the law and against morality not only in Western countries, although a woman of age and able to consent may sometimes think differently. The procedure may have serious physical and emotional consequences for the girl or woman. Nevertheless there are attempts to justify the procedure with medical and hygienic pseudoarguments, ideology, freedom of religion, cultural identity and social adequacy. Outside the Western world, some people claim that religion and culture alone justify the practice. In Germany, parents can lose the right to determine the residence of their daughter, if she is faced with the risk of genital mutilation in order to prevent that the child or girl is taken to her home country. Genital mutilation as a gender-specific threat is recognized as a reason to grant asylum or prevent deportation. Proposals to make genital mutilation a separate punishable offence are presently discussed by the legislator.     

Wine, fruit, and emission reductions: the CDM as development strategy in Chile

Through the Clean Development Mechanism (CDM) of the Kyoto Protocol, a developed country actor with a binding emission reduction target can use emission reductions from a project implemented in a developing country to meet this target. At the same time, CDM projects are meant to contribute to sustainable development in the host country, as defined by this country. The CDM is often taken to illustrate a broader contemporary turn in environmental policymaking toward market liberalism, flexibility, and pluralism, where the governance of the CDM involves ??agency beyond the state?? at different political levels and across various jurisdictions. While such an image of the CDM certainly identifies important aspects, it also downplays the ways in which states govern the CDM, not at the international level but at the domestic level through the host country prerogative to define its sustainable development priorities. Early on in the scholarly debate on the CDM, fears were raised about a ??race to the bottom?? with regard to sustainable development requirements on CDM projects. Our research on Chile confirms that hypothesis, with the important addition, that the ??race?? is not simply a structural feature of the CDM, but a deliberate strategy. We argue that Chile has actively chosen to put only marginal emphasis on securing the CDM??s contribution to sustainable development, and instead uses the CDM primarily as a tool to attract foreign investments, treating carbon credits as just another export product.     

Evaluation of amylase testing as a tool for saliva screening of crime scene trace swabs

Amylase testing has been used as a presumptive test for crime scene saliva for over three decades, mainly to locate saliva stains on surfaces. We have developed a saliva screening application for crime scene trace swabs, utilising an amylase sensitive paper (Phadebas^® Forensic Press test). Positive results were obtained for all tested dried saliva stains (0.5–32 μL) with high or intermediate amylase activity (840 and 290 kU/L). Results were typically obtained within 5 min, and all samples that produced DNA profiles were positive. However, salivary amylase activities, as well as DNA concentrations, vary significantly between individuals. We show that there is no correlation between amylase activity and amount of DNA in fresh saliva. Even so, a positive amylase result indicates presence of saliva, and thereby presence of DNA. Amylase testing may be useful for screening in investigations where the number of DNA analyses is limited due to cost, e.g., in volume crime.     

Geoengineering and global warming: a strategic perspective

If major emitters fail to mitigate global warming, they may have to resort to geoengineering techniques that deflect sunlight from planet Earth and remove carbon dioxide from the atmosphere. In this article, I develop a strategic theory of geoengineering. I emphasize two key features of geoengineering. First, whereas emissions reductions can be mandated now, geoengineering techniques are only available in the future. Second, major powers can unilaterally implement geoengineering projects that may hurt other countries. My game-theoretic analysis demonstrates that unilateral geoengineering presents a difficult governance problem if it produces negative externalities in foreign countries. Interestingly, countries may be tempted to reduce emissions now, so as to prevent a harmful geoengineering race in the future. The theoretical results can help scholars and policymakers understand the relationship between climate mitigation and geoengineering.     

Comparison of five DNA quantification methods

Six commercial preparations of human genomic DNA were quantified using five quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler™ Human DNA Quantification kit and RB1 rt-PCR. All methods measured higher DNA concentrations than expected based on the information by the manufacturers. UV spectrometry, SYBR-Green dye staining, slot blot and RB1 rt-PCR gave 39, 27, 11 and 12%, respectively, higher concentrations than expected based on the manufacturers’ information. The DNA preparations were quantified using the Quantifiler™ Human DNA Quantification kit in two experiments. The measured DNA concentrations with Quantifiler were 125 and 160% higher than expected based on the manufacturers’ information. When the Quantifiler™ human DNA standard (Raji cell line) was replaced by the commercial human DNA preparation G147A (Promega) to generate the DNA standard curve in the Quantifiler™ Human DNA Quantification kit, the DNA quantification results of the human DNA preparations were 31% higher than expected based on the manufacturers’ information. The results indicate a calibration problem with the Quantifiler™ human DNA standard for its use with the Quantifiler™ Human DNA Quantification kit. The possible reasons for the problem are discussed and a solution is suggested. The results emphasise the need for standard reference DNA material and standard methods for DNA quantification.     

Between pedagogy and practice: developing and delivering international justice coursework in North America

While the need for new and innovative international coursework in North America has been recognized, developments in North American Universities have been few. Building on Mathias Reimann’s (Penn State International Law Review 22:397–415 [23]) useful justification for a new basic course in international law, this paper provides a discussion of a new approach to international justice coursework. Based on the nascent ICC and the jurisprudential developments of past international tribunals, a discrete international legal order has emerged. This may provide an opportunity for a broader examination of international criminal justice drawn from disciplines such as history, sociology, psychology, and criminology. By offering practical classroom techniques to assist in the delivery of international justice coursework, this paper attempts to provoke debate and discussion about how international coursework ought to be designed and delivered to a wider audience of undergraduate students.     

Fiscal Decentralization and Intergovernmental Transfers in Less Developed Countries

This article addresses the issue of fiscal decentralizationin developing countries, and the use of intergovernmental transfersto achieve this objective. We find that developing countrieshave more centralized fiscal structures and argue that thisis consistent with the theory of fiscal federalism. Economicdevelopment, however, does push the advantage toward decentralization.We also show that developing countries use a wide variety oftransfer instruments to fund local governments, and that theseinstruments give the national government varying degrees ofcontrol over local government finances.     

Contests with an unknown number of contestants

This paper studies rent-seeking contests where the contestants do not know the number of actively competing contestants. Two models are compared. In the first, all players are risk neutral; in the second, all have constant absolute risk aversion. If the expected fraction of active contestants is low, an increase in the number of potential contestants increases individual rent-seeking efforts. This effect is in contrast to the complete information case where individual rent-seeking efforts decrease in the number of contestants. The effect is more likely under risk neutrality, but also possible under risk aversion. Equilibrium rent seeking efforts are lower under risk aversion if and only if the expected fraction of active contestants is low.